Microfluidic devise for real-time analysis of particulate allergen in an environment

• Project/Area Number: 22760295
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Measurement engineering
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ARAKAWA Takahiro 東京医科歯科大学, 生体材料工学研究所, 助教 (50409637)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
• Keywords: マイクロ流体デバイス / マイクロ流体システム / マイクロ流路 / 三次元構造体 / PDMS / MEMS技術 / アレルゲン / 環境計測

Research Abstract

A microfluidic device for measuring of allergenic substances was fabricated and demonstrated. From the environmental points of view, a continuous measurement method of allergenic substances with high sensitivity and selectivity is strongly requested. Measurement range of ng/ml-level was established in this system. This microfluidic system allows us to evaluate various substances for an environmental monitoring.

Research Products

• [Journal Article] Biosensor for L-phenylalanine based on the optical detection of NADH using a UV light emitting diode (2011)
  • Author(s): Arakawa T, Koshida T, Gessei T, Takahashi D, Kudo H, Yano K, Mitsubayashi K.
  • Journal Title: Microchimica Acta Volume: 173(1-2) Pages: 199-205
• [Journal Article] High-throughput single-cell manipulation system for a arge number of target cells (2011)
  • Author(s): Arakawa T, Noguchi M, Sumitomo K, Yamaguchi Y, Shoji S.
  • Journal Title: Biomicrofluidics Volume: 5 Pages: 14114-14114
• [Journal Article] Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae(Der f 1) (2011)
  • Author(s): Miyajima K, Itabashi G, Koshida T, Tamari K, Takahashi D, Arakawa T, Kudo H, Saito H, Yano K, Shiba K, Mitsubayashi K.
  • Journal Title: Environmental Monitoring and Assessment Volume: 182(1-4) Pages: 233-241
• [Journal Article] High-throughput single-cell manipulation system for a large number of target cells (2011)
  • Author(s): Arakawa T, Noguchi M, Sumitomo K, Yamaguchi Y, Shoji S
  • Journal Title: Biomicrofluidics Volume: 5 Pages: 14114-14114
  • Peer Reviewed
• [Journal Article] Fluorescence immunoassay using an optical fiber for determination of Dermatophagoides farinae (Der f 1) (2011)
  • Author(s): Miyajima K, Itabashi G, Koshida T, Tamari K, Takahashi D, Arakawa T, Kudo H, Saito H, Yano K, Shiba K, Mitsubayashi K
  • Journal Title: Environmental Monitoring and Assessment Volume: 182(1-4) Pages: 233-241
  • Peer Reviewed
• [Journal Article] Real-time chemiluminescence visualization system of spacially-distributed exhausted ethanol breath on enzyme immobilized mesh substrate (2010)
  • Author(s): Arakawa T, Wang X, Ando E, Endo H, Takahashi D, Kudo H, Saito H, Mitsubayashi K.
  • Journal Title: Luminescence Volume: 25 Pages: 185-187
• [Journal Article] Fluorescence biosensing system with a UV-LED excitation for 1-leucine detection (2010)
  • Author(s): Koshida T, Arakawa T, Gessei T, Takahashi D, Kudo H, Saito H, Yano K, Mitsubayashi K.
  • Journal Title: Sensors & Actuators B Chemical Volume: 146(1) Pages: 177-182
• [Journal Article] Fluorescence biosensing system with a UV-LED excitation for l-leucine detection (2010)
  • Author(s): Koshida T, Arakawa T, Gessei T, Takahashi D, Kudo H, Saito H, Yano K, Mitsubayashi K
  • Journal Title: Sensors & Actuators B Chemical Volume: 146(1)211 Pages: 177-182
  • Peer Reviewed
• [Presentation] A drug-injection system with chemo-mechanical energy conversion for active feedback control of glucose concentration in a blood (2011)
  • Author(s): Arakawa T, Kato R, Munkhjargal M, Takahashi D, Kudo H, Mitsubayashi K
  • Organizer: The 16th International Conference on Solid-State Sensors, Actuators and Microsystems
  • Place of Presentation: Beijing, China
• [Presentation] Bio-inspired self-regulation system of glucose level with chemo-mechanical energy conversion bio-membrane (2011)
  • Author(s): Munkhjargal M, Kato R, Miyajima K, Arakawa T, TKudo H, Mitsubayashi K
  • Organizer: International Bionic Engineering Conference 2011
  • Place of Presentation: Boston, USA
• [Presentation] 機能性タンパク質を利用したモニタリングシステムに関する研究 (2011)
  • Author(s): 荒川貴博,北和昂,王析,宮島久美子,高橋大志,工藤寛之,三林浩二
  • Organizer: 第28回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 東京
• [Presentation] Active feedback drug-injection system consisting of decompression unit and drug release unit with chemo-mechanical energy conversion (2011)
  • Author(s): Arakawa T, Kato R, Hatayama K, Munkhjargal M, Kudo H, Mitsubayashi K
  • Organizer: 2nd International Conference on Bio-Sensing Technology 2011
  • Place of Presentation: Amsterdam, The Netherlands
• [Presentation] 生体触媒を利用した呼気中エタノールガスの二次元可視化計測システムに関する研究 (2011)
  • Author(s): 荒川貴博,王シン,高橋大志,工藤寛之,三林浩二
  • Organizer: 第20回インテリジェント材料/システムシンポジウム
  • Place of Presentation: 東京
• [Presentation] A drug-injection system with chemo-mechanical energy conversion for active feedback control of glucose concentration in a blood (2011)
  • Author(s): Takahiro Arakawa
  • Organizer: The 16^<th> International Conference on Solid-state Sensors, Actuators and Microsystems
  • Place of Presentation: 北京中国
• [Presentation] Intelligent kinetic biosensors with bionic pressure system (2011)
  • Author(s): Takahiro Arakawa
  • Organizer: International Bionic Engineering Conference 2011
  • Place of Presentation: Boston USA
• [Presentation] 機能性タンパク質を利用したモニタリングシステムに関する研究 (2011)
  • Author(s): 荒川貴博
  • Organizer: 第28回「センサ・マイクロマシンと応用システム」シンポジウム
  • Place of Presentation: 船堀東京
• [Presentation] Direct imaging of exhausted breath ethanol on a novel chemiluminescence method using enzyme-immobilized mesh (2011)
  • Author(s): Takahiro Arakawa
  • Organizer: 2nd International Conference on Bio-Sensing Technology 2011
  • Place of Presentation: Amsterdam Netherlands
• [Presentation] 生体触媒を利用した呼気中エクノールガスのリアルタイム二次元可視化計測システム (2011)
  • Author(s): 荒川貴博
  • Organizer: 第20回インテリジェント材料/システムシンポジウム
  • Place of Presentation: 東京
• [Presentation] 生体触媒を利用した呼気中エタノールガス計測のための可視化システムに関する研究 (2010)
  • Author(s): 荒川貴博,王シン,安藤恵理,北和昂,高橋大志,工藤寛之,齊藤浩一,三林浩二
  • Organizer: 2010年・秋季<第71回>応用物理学会学術講演会
  • Place of Presentation: 長崎
• [Presentation] Mitsubayashi K. A chemiluminescent visualization system for breath ethanol monitoring using enzyme-immobilized substrate (2010)
  • Author(s): Wang X, Arakawa T, Ando E, Endo H, Takahashi D, Saito H, Kudo H
  • Organizer: 13th international meeting of chemical sensors(IMCS-13)
  • Place of Presentation: Perth, Western Australia
• [Presentation] A chemiluminescent visualization system for breath ethanol monitoring using enzyme-immobilized substrate (2010)
  • Author(s): T.Arakawa
  • Organizer: IMCS-13
  • Place of Presentation: Perth, Autsralia
• [Presentation] 生体触媒を利用した呼気中エタノールガス計測のための可視化システムに関する研究 (2010)
  • Author(s): 荒川貴博
  • Organizer: 秋季<第71回>応用物理学会学術講演会
  • Place of Presentation: 長崎
• [Remarks] センサEXPO2010、東京ビックサイト、2010年11月
• [Remarks] センサEXPO2011、東京ビックサイト、2011年11月