| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
|
|---|
| Research Abstract |
To construct next generation of biocatcher specific for rare metal, we attempted to endow adsorption selectivity with biocatcher constructed by cell surface engineering. Six amino acid residues involving metal binding in the ModE, molybdate-binding protein from Escherichia coli, were substituted with other amino acids, and the ModE mutants were displayed on yeast cell surface. Among the ModE mutant-displaying yeasts, T163Y mutant-displaying yeast showed the selective adsorption of tungstate. In addition, to construct Li^+-adsorbing yeast, cell surface display of Li^+-binding proteins and screening of Li^+-binding peptides from random peptide library were attempted. Screening of Li^+-binding peptides from yeast library displaying combinatorial random peptides, which consisted of 25 amino acid residues, was performed on the basis of Li^+tolerance. As a result, Li^+tolerant yeast was acquired, and there is the possibility that tolerant yeast could adsorb Li^+by the displayed peptide.
|
