Economical and Efficient Production of Cardiac Myocytes Using Small Molecule-Responsive Artificial Receptors For Wnt3a Signal Transduction

• Project/Area Number: 22760607
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kyoto University
• Principal Investigator: SOGO Takahiro 京都大学, 生命科学系・キャリアパス形成ユニット, 研究員 (30561972)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
• Keywords: 心筋再生 / 幹細胞 / シグナル伝達 / 受容体 / 抗体 / Wnt3a / ES細胞 / iPS細胞

Research Abstract

We constructed chimeric receptors in which single-chain Fv of anti-fluorescein(FL) antibody was tethered to transmembrane/cytoplasmic domains of Frizzled8 and LRP6 that are receptors for Wnt3a. Mouse ES cells or iPS cells transduced with these chimeric receptors could activate Wnt3a signaling in response to FL-conjugated BSA(BSA-FL) as a cognate ligand, and could efficiently differentiate into cardiomyocyte when they are stimulated with BSA-FL, in the absence of Wnt3a.

Research Products

• [Presentation] Efficient Production of Cardiac Myocytes from EScells and iPS cells Using Small Molecule-responsive Artificial Receptors for Wnt3a Signal Transduction (2012)
  • Author(s): Sogo T and Kawamura T
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡県福岡市福岡国際会議場
  • Year and Date: 2012-03-18
• [Presentation] Efficient Production of Cardiac Myocytes from ES cells and iPS cells Using Small Molecule-responsive Artificial Receptors for Wnt3a Signal Transduction (2012)
  • Author(s): Sogo T, Kawamura T
  • Organizer: 第76回日本循環器学会学術集会
  • Place of Presentation: 福岡市、福岡国際会議場
  • Year and Date: 2012-03-18
• [Presentation] Economical and Efficient Production of Cardiac Myocytes From ESCells and iPS Cells Using Small Molecule-Responsive Artificial Receptors For Wnt3a Signal Transduction (2011)
  • Author(s): Sogo T, Shigeno A, Maruno T, Hasegawa K, Kawahara M, Ueda H, Nagamune T, Kawamura T
  • Organizer: American Heart Association Scientific Sessions 2011
  • Place of Presentation: アメリカ合衆国フロリダ州オーランド
  • Year and Date: 2011-11-16
• [Presentation] Economical and Efficient Production of Cardiac Myocytes From ES Cells and iPS Cells Using Small Molecule-Responsive Artificial Receptors For Wnt3a Signal Transduction (2011)
  • Author(s): Sogo T, Shigeno A, Maruno T, Hasegawa K, Kawahara M, Ueda H, Nagamine T, Kawamura T
  • Organizer: American Heart Association, Scientific Sessions 2011
  • Place of Presentation: アメリカ合衆国フロリダ州オーランド
  • Year and Date: 2011-11-16
• [Presentation] 経済的かつ効率的な心筋再生療法のための、Wnt3aシグナルを伝達する人工受容体の作製 (2011)
  • Author(s): 十河孝浩、重野麻子、丸野敬晃、長谷川浩二、河原正浩、上田宏、長棟輝行、川村晃久
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都府京都市国立京都国際会館
  • Year and Date: 2011-09-24
• [Presentation] 古典的Wnt経路を伝達する人工受容体を用いた経済的な心筋再生療法の確立 (2011)
  • Author(s): 十河孝浩、重野麻子、丸野敬晃、長谷川浩二、河原正浩、上田宏、長棟輝行、川村晃久
  • Organizer: 化学工学会第43回秋季大会
  • Place of Presentation: 愛知県名古屋市名古屋工業大学
  • Year and Date: 2011-09-24
• [Presentation] Efficient creation of induced pluripotent stem cells for myocardial regeneration therapy. (2010)
  • Author(s): Sogo T, Takaya T, Shigeno A, Kojima Y, Hasegawa K, Kawamura T.
  • Organizer: World Heart Federation
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-06-19
• [Presentation] Role of Wnt signaling in cardiac differentiation from mouse embryonic stem cells and induced pluripotent stem cells. (2010)
  • Author(s): Shigeno A, Sogo T, Takaya T, Kojima Y, Hasegawa K, Kawamura T.
  • Organizer: World Heart Federation
  • Place of Presentation: Beijing, China
  • Year and Date: 2010-06-18
• [Book] アンチ・エイジング医学-日本抗加齢医学会雑誌 (2011)
  • Author(s): 川村晃久、十河孝浩
  • Publisher: メディカルビュー社
• [Book] アンチ・エイジング医学-日本抗加齢医学会雑誌 (2011)
  • Author(s): 川村晃久、十河孝浩
  • Total Pages: 6
  • Publisher: メディカルレビュー社
• [Book] アンチ・エイジング医学-日本抗加齢医学会雑誌
  • Author(s): 川村晃久、十河孝浩
  • Total Pages: 6
  • Publisher: メディカルビュー社
• [Remarks]
  • URL: http://www.cp.kyoto-u.ac.jp/
• [Remarks]
  • URL: http://www.cp.kyoto-u.ac.jp/