Development of value-added chemicals from light using light-driven vesicles and cell factory.
| Project/Area Number |
22760608
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kobe University |
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Principal Investigator |
HARA Kiyotaka 神戸大学, 自然科学系先端融合研究環, 准教授 (40434378)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
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| Keywords | ATP / ATP synthase / Membrane vesicle / Photoenergetics / Rhodopsin / Vesicles / ＡＴＰ / 光利用 / 出芽酵母 / 酸化・還元エネルギー / エネルギー計測 / 光 / バイオプロセス / 大腸菌 / 好熱菌 / 反転膜 / ロドプシン / ATP合成酵素 |
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| Research Abstract |
We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H+ transporter) and TFoF1-ATP synthase (a thermostable ATP synthase). These vesicles showed lightdependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATPregenerating bioprocess for various ATP-hydrolyzing bioproductions.
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Report
(5 results)
Research Products
(18 results)
