Development of value-added chemicals from light using light-driven vesicles and cell factory.

• Project/Area Number: 22760608
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: Kobe University
• Principal Investigator: HARA Kiyotaka 神戸大学, 自然科学系先端融合研究環, 准教授 (40434378)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: ATP / ATP synthase / Membrane vesicle / Photoenergetics / Rhodopsin / Vesicles / ＡＴＰ / 光利用 / 出芽酵母 / 酸化・還元エネルギー / エネルギー計測 / 光 / バイオプロセス / 大腸菌 / 好熱菌 / 反転膜 / ロドプシン / ATP合成酵素

Research Abstract

We prepared ATP photosynthetic vesicles from inside-out membranes of Escherichia coli cells that express delta-rhodopsin (a novel light-driven H+ transporter) and TFoF1-ATP synthase (a thermostable ATP synthase). These vesicles showed lightdependent ATP synthesis. Furthermore, coupling the ATP photosynthetic vesicles with an ATP-hydrolyzing hexokinase enabled light-dependent glucose consumption. The ATP photosynthetic vesicles indicate their potential to applied to light-driven ATPregenerating bioprocess for various ATP-hydrolyzing bioproductions.

Research Products

• [Journal Article] Construction of photoenergetic mitochondria in cultured mammalian cells (2013)
  • Author(s): Kiyotaka Y. Hara, Takeyoshi Wada, Kuniki Kino, Toru Asahi, Naoya Sawamura
  • Journal Title: Scientific Report Volume: s3 Pages: 1635-1635
  • Peer Reviewed
• [Journal Article] Oxidized glutathione fermentation using Saccharomyces cerevisiae engineered for glutathione metabolism. (2013)
  • Author(s): Kiriyama K, Hara KY, Kondo A.
  • Journal Title: Appl Microbiol Biotechnol. Volume: 97 Issue: 16 Pages: 7399-7404
  • DOI: 10.1007/s00253-013-5074-8
  • Peer Reviewed
• [Journal Article] A review of enzymes and microbes for lignocellulosic biorefinery and the possibility of their application to consolidated bioprocessing technology. (2013)
  • Author(s): Hasunuma T, Okazaki F, Okai N, Hara KY, Ishii J, Kondo A.
  • Journal Title: Bioresour Technol. Volume: 135 Pages: 513-522
  • DOI: 10.1016/j.biortech.2012.10.047
  • Peer Reviewed
• [Journal Article] Extracellular glutathione fermentation using engineered Saccharomyces cerevisiae expressing novel glutathione exporter (2012)
  • Author(s): Kentaro Kiriyama
  • Journal Title: Applied Microbiology and Biotechnology Volume: 96(4), Issue: 4 Pages: 1021-1027
  • DOI: 10.1007/s00253-012-4075-3
  • Peer Reviewed
• [Journal Article] ATP photosynthetic vesicles for light-driven bioprocesses (2011)
  • Author(s): Kiyotaka Y. Hara, Rie Suzuki, Toshiharu Suzuki, Masasuke Yoshida, Kuniki Kino
  • Journal Title: Biotechnology Letters Volume: 33(6) Pages: 1133-1138
  • Peer Reviewed
• [Journal Article] Enzymatic glutathione production using metabolically engineered Saccha romyces cerevisiae as a whole-cell biocatalyst (2011)
  • Author(s): Yoshida H, Hara KY, Kiriyama K, Nakayama H, Okazaki F, Matsuda F, Ogino C, Fukuda H, Kondo A
  • Journal Title: Applied Microbiology and Biotechnology Volume: 91 Issue: 4 Pages: 1001-1006
  • DOI: 10.1007/s00253-011-3196-4
  • Peer Reviewed
• [Journal Article] ATP Photosynthetic vesicles for light-driven bioprocesses. (2011)
  • Author(s): Hara KY, Suzuki R, Suzuki T, Yoshida M, Kino K.
  • Journal Title: Biotechnolology Letters Volume: (掲載確定)
  • Peer Reviewed
• [Presentation] 合成生物工学を用いた高効率有用物質生産細胞の創製 (2013)
  • Author(s): 原清敬, 桐山健太郎, 近藤昭彦
  • Organizer: 細胞を創る研究会
  • Place of Presentation: 慶応大学鶴岡キャンパス
  • Year and Date: 2013-11-14
• [Presentation] 出芽酵母を用いたグルタチオンの生産 (2013)
  • Author(s): 原清敬
  • Organizer: 平成25年度開発型企業連携研究会
  • Place of Presentation: 神戸大学
  • Year and Date: 2013-07-04
• [Presentation] 光駆動ATP再生小胞の創製 (2012)
  • Author(s): 原清敬
  • Organizer: 第45回原生動物学会
  • Place of Presentation: 兵庫県立大学書写キャンパス
  • Year and Date: 2012-11-23
• [Presentation] 新規グルタチオン排出トランスポーター発現酵母を用いた細胞外グルタチオン生産 (2012)
  • Author(s): 原清敬, 桐山健太郎, 福田秀樹, 近藤昭彦
  • Organizer: 第7回トランスポーター研究会
  • Place of Presentation: 京都大学
  • Year and Date: 2012-06-09
• [Presentation] 出芽酵母における新規グルタチオン排出ABCトランスポーターの同定 (2012)
  • Author(s): 原 清敬
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: マリンメッセ福岡
• [Presentation] 酵母によるバイオマスからのグルタチオンの直接発酵 (2011)
  • Author(s): 原清敬, 吉田秀世, 桐山健太郎, 金松希, 荻野千秋, 近藤昭彦
  • Organizer: 第63回日本生物工学会
  • Place of Presentation: 東京農工大学
  • Year and Date: 2011-09-27
• [Presentation] 代謝改変酵母を用いた開放系菌体触媒によるグルタチオン生産 (2011)
  • Author(s): 原清敬、吉田秀世、近藤昭彦
  • Organizer: 酵素工学研究会第66回講演会
  • Place of Presentation: 東京大学山上会館(東京都)
  • Year and Date: 2011-09-02
• [Presentation] 合成生物工学を用いた高効率有用物質生産細胞の創製
  • Author(s): 原清敬、桐山健太郎、近藤昭彦
  • Organizer: 「細胞を創る」研究会6.0
  • Place of Presentation: 鶴岡メタボロームキャンパス
  • Invited
• [Presentation] 出芽酵母を用いたグルタチオンの生産
  • Author(s): 原清敬
  • Organizer: 化学工学会関西支部 開発型企業連携研究会
  • Place of Presentation: 神戸大学
  • Invited
• [Remarks]
  • URL: http://www2.kobe-u.ac.jp/~akondo/
• [Remarks]
  • URL: http://www.org.kobe-u.ac.jp/bioproduction/