Construction of self-assembled protein-based microsphere having molecular recognition ability.

• Project/Area Number: 22760615
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute for Materials Science
• Principal Investigator: YAMAZAKI Tomohiko 独立行政法人物質・材料研究機構, 国際ナノアーキテクトニクス研究拠点, MANA研究者 (50419264)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 分子認識 / 酵素 / 両親媒性蛋白質 / ハイドロフォビン / マイクロ粒子 / 徐放制御 / ソルターゼ / 分子認識ペプチド

Research Abstract

We constructed self-assembled membranes and particles using amphipathic protein, hydrophobins. We, then, established the enzyme based anchoring methods to connect biological molecules, such as DNA aptamer, peptide and protein, with amphipathic protein-based self-assembly membrane and particle. The system can be utilized for construction protein microsphere having molecular recognition ability. Furthermore, this protein based microsphere can be applied for an active drug release system induced by biomarker molecule in human body.

Research Products

• [Journal Article] Influence of template/functional monomer/cross-linking monomer ratio on particle size and binding properties of molecularly imprinted nanoparticles (2012)
  • Author(s): K. Yoshimatsu, T. Yamazaki, I. Chronakis, L. Ye
  • Journal Title: Journal of Applied Polymer Science Volume: 124 Issue: 2 Pages: 1249-1255
  • DOI: 10.1002/app.35150
  • Peer Reviewed
• [Journal Article] Protein engineering approaches for constructing novel glucose sensing elements (2010)
  • Author(s): T. Yamazaki and A. Sakaguchi
  • Journal Title: Indonesian Nanoletter Volume: 3 Pages: 44-46
  • Peer Reviewed
• [Presentation] 遺伝性肝疾患治療を目指した肝臓細胞への遺伝子導入 (2012)
  • Author(s): 山崎智彦,辰巳公平,大橋一夫,谷口彰良,岡野光夫
  • Organizer: 第21回インテリジェント材料・システムシンポジウム
  • Place of Presentation: 東京(日本)
  • Year and Date: 2012-01-10
• [Presentation] Engineered transcriptional regulators for intracellular sensing and gene regulation (2012)
  • Author(s): 山崎智彦, 辰巳公平, 大橋一夫, 谷口彰良, 岡野光夫
  • Organizer: 第21回インテリジェント材料・システムシンポジウム
  • Place of Presentation: 東京女子医科大学(東京都)
  • Year and Date: 2012-01-10
• [Presentation] 遺伝子銃を用いた肝臓細胞への遺伝子導入 (2011)
  • Author(s): 山崎智彦,辰巳公平,吉松啓一, Wang Dan,大橋一夫,谷口彰良,岡野光夫
  • Organizer: 第33回日本バイオマテリアル学会大会
  • Place of Presentation: 京都(日本)
  • Year and Date: 2011-11-22
• [Presentation] 遺伝子銃を用いた肝臓細胞への遺伝子導入 (2011)
  • Author(s): 山崎智彦, 辰巳公平, 吉松啓一, Wang Dan, 大橋一夫, 谷口彰良, 岡野光夫
  • Organizer: 第33回日本バイオマテリアル学会大会
  • Place of Presentation: 京都テルサ(京都府)
  • Year and Date: 2011-11-22
• [Presentation] Engineered transcriptional regulators for intracellular sensing and gene regulation (2011)
  • Author(s): T. Yamazaki, A. Mikami, K. Sode, A. Taniguchi, N. Hanagata
  • Organizer: Japan-China International Forum of Advanced Research on Biotechnology 2011
  • Place of Presentation: 東京(日本)
  • Year and Date: 2011-11-10
• [Presentation] Engineered transcriptional regulators for intracellular sensing and gene regulation (2011)
  • Author(s): 山崎智彦, 坂口あかね, 早出広司, 谷口彰良, 花方信孝
  • Organizer: 日中バイオテクノロジー先端フォーラム2011
  • Place of Presentation: 東京海洋大学(東京都)(招待講演)
  • Year and Date: 2011-11-10
• [Presentation] Novel CpG phosphodiester oligodeoxynucleotides as human toll-like receptor 9 agonists (2011)
  • Author(s): T. Yamazaki, W. Meng, N. Hanagata
  • Organizer: Infection & Immunity
  • Place of Presentation: Suzhou(中国)
  • Year and Date: 2011-09-11
• [Presentation] Novel CpG phosphodiester oligodeoxynucleotides as human toll-like receptor 9 agonists (2011)
  • Author(s): 山崎智彦, 孟文君, 花方信孝
  • Organizer: Infection & Immunity
  • Place of Presentation: Suzhou Dushu Lake Conference Center(蘇州,中国)
  • Year and Date: 2011-09-11
• [Presentation] Identification of Hydroxyapatite-Binding Peptides (2011)
  • Author(s): T. Yamazaki, N. Hanagata
  • Organizer: Joint Conference of The Fifth International Conference on the Science and Technology for Advanced Ceramics(STAC-5) and The 2nd International Conference
  • Place of Presentation: 横浜(日本)
  • Year and Date: 2011-06-22
• [Presentation] Identification of Hydroxyapatite-Binding Peptides (2011)
  • Author(s): 山崎智彦, 花方信孝
  • Organizer: STAC5-AMDI2
  • Place of Presentation: メルパルク横浜(神奈川県)
  • Year and Date: 2011-06-22
• [Presentation] Screening of Hydroxyapatite-Binding Peptides using Phage Display Library (2010)
  • Author(s): T. Yamazaki, Y. Nisida, A. Taniguchi, T. Ikoma
  • Organizer: Fourth International Conference on Science and Technology of Advanced Ceramics
  • Place of Presentation: 横浜(日本)
  • Year and Date: 2010-06-21
• [Presentation] Screening of Hydroxyapatite-Binding Peptides using Phage Display Library (2010)
  • Author(s): 山崎智彦、西田有紀、谷口彰良、生駒俊之
  • Organizer: Fourth International Conference on Fourth International Conferen
  • Place of Presentation: メルパルクホール,横浜
  • Year and Date: 2010-06-21
• [Remarks]
  • URL: http://www.nims.go.jp/bmc/group/control/GBSCUM/index.html