Development of a new strategy for protein design based on a systematic mutant database

Research Project

Project/Area Number	22760616
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Biofunction/Bioprocess
Research Institution	National Institute of Advanced Industrial Science and Technology
Principal Investigator	YOKOTA Akiko 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (20415764)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000) Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000) Fiscal Year 2010: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Keywords	蛋白質 / 酵素 / 機能改変 / 変異解析
Research Abstract	We have aimed to establish a useful and reliable new method for protein engineering through acquirement of empirical rules towards rational design and development of a scheme based on directed evolution, by using a comprehensive mutant database of dihydrofolate reductase(DHFR) as a model protein. Piecing out and improving the database, we obtained the more precise data about relationship between the amino acid sequence and protein characteristic parameters, which would supply newly desirable information for protein design.