A buildup approach to the design of a small-sized protein via an autonomously-folded short segment
| Project/Area Number |
22760617
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Biofunction/Bioprocess
|
|---|
| Research Institution | National Institute of Advanced Industrial Science and Technology |
|---|
Principal Investigator |
WATANABE Hideki 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (90422089)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Keywords | 生物機能工学 / 人工蛋白質 / 分子進化工学 / 抗体医薬 / ファージディスプレイ |
|---|
| Research Abstract |
This study established a methodology for the design of small-sized proteins on the basis of segment elongation by using chignolin that was introduced as a structural support. Using T7 phage displayed library, we selected artificial proteins with affinity for antibody Fc region that was used as a model target. Function and three dimensional structures of artificial proteins were analyzed in detail by using surface plasmon resonance, NMR, and X-ray crystallography.
|
Report
(3 results)
Research Products
(9 results)
