Development of novel cell-based assay chip using microfluidics

• Project/Area Number: 22760618
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biofunction/Bioprocess
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: SUGIURA Shinji 独立行政法人産業技術総合研究所, 幹細胞工学研究センター, 主任研究員 (10399496)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 医用化学工学 / マイクロフルイディクス / 細胞アッセイ / 灌流培養 / 細胞毒性試験 / バイオMEMS / マイクロプロセス工学 / 創薬スクリーニング / 移動速度論

Research Abstract

We developed a perfusion culture microchamber array chip with a serial dilution microfluidic network for analyzing drug dose response, which is required for practical drug discovery applications. We used a mathematical model of transport phenomena to investigate the concentration profile of oxygen and substances in a perfusion cell-culture microchamber array, and obtained optimal design strategy of a microfluidic perfusion culture chip.

Research Products

• [Journal Article] Comparison of substance supply in static an perfusion cultures based on mass transport phenomena (2011)
  • Author(s): Sugiura, S. & Kanamori, T
  • Journal Title: Biochemical Engineering Journal Volume: 57 Pages: 69-74
  • DOI: 10.1016/j.bej.2011.08.010
  • Peer Reviewed
• [Journal Article] Superior oxygen and glucose supply in perfusion cell cultures compared to static cell cultures demonstrated by simulations using the finite element method (2011)
  • Author(s): Sugiura, S., Sakai, Y., Nakazawa, K. & Kanamori, T
  • Journal Title: Biomicrofluidics Volume: 5 Issue: 2 Pages: 22202-22202
  • DOI: 10.1063/1.3589910
  • Peer Reviewed
• [Journal Article] マイクロチップを用いた細胞アッセイ (2010)
  • Author(s): 杉浦慎治,服部浩二,金森敏幸
  • Journal Title: 化学センサ Volume: 26-4 Pages: 170-175
  • NAID: 40018709185
  • URL: http://chemsens.electrochem.jp/journal/2010/2010-4abt.htm
• [Journal Article] 細胞アッセイマイクロチップ (2010)
  • Author(s): 杉浦慎治,服部浩二,金森敏幸
  • Journal Title: 化学装置 Volume: 52-9 Pages: 2-6
  • URL: http://ci.nii.ac.jp/naid/40017264755
• [Journal Article] 細胞アッセイチップ開発における化学工学 (2010)
  • Author(s): 杉浦慎治,服部浩二,金森敏幸
  • Journal Title: 化学工学 Volume: 74-6 Pages: 294-294
  • NAID: 10026356846
  • URL: http://www.scej.org/content/view/949/13/
• [Journal Article] Microfluidic Serial Dilution Cell-Based Assay for Analyzing Drug Dose Response over a Wide Concentration Range (2010)
  • Author(s): Sugiura, S., Hattori, K. & Kanamori, T.
  • Journal Title: Anal. Chem Volume: 82 Issue: 19 Pages: 8278-8282
  • DOI: 10.1021/ac1017666
  • Peer Reviewed
• [Journal Article] Microfluidic Serial Dilution Cell-Based Assay for Analyzing Drug Dose Response over a Wide Concentration Range (2010)
  • Author(s): Shinji Sugiura, Koji Hattori, Toshiyuki Kanamori
  • Journal Title: Analytical Chemistry Volume: 82 Pages: 8278-8282
  • Peer Reviewed
• [Journal Article] 細胞アッセイチップ開発における化学工学 (2010)
  • Author(s): 杉浦慎治、服部浩二、金森敏幸
  • Journal Title: 化学工学 Volume: 74 Pages: 292-294
  • NAID: 10026356846
• [Journal Article] 細胞アッセイマイクロチップ (2010)
  • Author(s): 杉浦慎治、服部浩二、金森敏幸
  • Journal Title: 化学装置 Volume: 52 Pages: 2-6
• [Journal Article] マイクロチップを用いた細胞アッセイ (2010)
  • Author(s): 杉浦慎治、服部浩二、金森敏幸
  • Journal Title: 化学センサ Volume: 26 Pages: 170-175
  • NAID: 40018709185
• [Presentation] 医薬品アッセイへの応用を目指した細胞チップ開発 (2011)
  • Author(s): 杉浦慎治,須丸公雄,金森敏幸
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京
  • Year and Date: 2011-09-28
• [Presentation] 医薬品アッセイへの応用を目指した細胞チップ開発 (2011)
  • Author(s): 杉浦慎治、須丸公雄、金森敏幸
  • Organizer: 第63回日本生物工学会大会
  • Place of Presentation: 東京、東京農工大学(招待講演)
  • Year and Date: 2011-09-28
• [Presentation] Perfusion Culture Microchamber Array Chip for High-Throughput Cell-Based (2011)
  • Author(s): Sugiura, S., Hattori, K. & Kanamori, T.
  • Organizer: SBS17th Annual Conference & Exhibition
  • Place of Presentation: Orland
  • Year and Date: 2011-03-28
• [Presentation] Perfusion Culture Microchamber Array Chip for High-Throughput Cell-Based Assay (2011)
  • Author(s): Shinji Sugiura, Koji Hattori, Toshiyuki Kanamori
  • Organizer: SBS 17th Annual Conference & Exhibition
  • Place of Presentation: Orland
• [Presentation] 医薬品アッセイへの応用を目指した細胞チップ開発 (2010)
  • Author(s): 金森敏幸,杉浦慎治,服部浩二,須丸公雄
  • Organizer: 動物実験代替法学会第23回大会
  • Place of Presentation: 東京
  • Year and Date: 2010-12-05
• [Presentation] 灌流培養マイクロチャンバーアレイと段階希釈流路網を集積化した細胞アッセイチップの開発 (2010)
  • Author(s): 服部浩二,杉浦慎治,藤橋政人,黒川正也,中野優一,柳澤真澄,金森敏幸
  • Organizer: 第21回化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 名古屋
  • Year and Date: 2010-11-28
• [Presentation] Integrated Perfusion Culture Microchamber Array Chip for High-Throughput Drug Dose Response Assay (2010)
  • Author(s): Sugiura, S., Hattori, K. & Kanamori, T.
  • Organizer: 218th Meeting of the Electrochemical Society
  • Place of Presentation: Las Vegas
  • Year and Date: 2010-10-13
• [Presentation] マイクロプロセスを利用した細胞マニピュレーション/アッセイシステムの開発 (2010)
  • Author(s): 杉浦慎治,服部浩二,須丸公雄,金森敏幸
  • Organizer: 化学工学会第42回秋季大会
  • Place of Presentation: 京都
  • Year and Date: 2010-09-06
• [Presentation] Integrated Pressure Driven Perfusion Culture Microchamber Array Chip For High-Throughput Drug Dose Response Assay (2010)
  • Author(s): Sugiura, S., Hattori, K., Fujihashi, M., Kurokawa, M., Nakano, Y., Yanagisawa, M., & Kanamori, T.
  • Organizer: The International Symposium on Microchemistry and Microsystems(ISMM) 2010
  • Place of Presentation: Hong Kong
  • Year and Date: 2010-05-28
• [Presentation] 灌流培養マイクロチャンバーアレイチップによる薬物毒性評価 (2010)
  • Author(s): 杉浦慎治,服部浩二,金森敏幸
  • Organizer: 第17回HAB研究機構学術年会
  • Place of Presentation: 東京
  • Year and Date: 2010-05-22
• [Presentation] Perfusion Culture Microchamber Array Chip for High Throughput Drug Dose Response Assay (2010)
  • Author(s): Sugiura, S., Hattori, K. & Kanamori, T.
  • Organizer: SBS16th Annual Conference & Exhibition
  • Place of Presentation: Phoenix
  • Year and Date: 2010-04-12
• [Presentation] Perfusion Culture Microchamber Array Chip for High Throughput Drug Dose Response Assay (2010)
  • Author(s): Shinji Sugiura, Koji Hattori, Toshiyuki Kanamori
  • Organizer: SBS 16th Annual Conference & Exhibition
  • Place of Presentation: Phoenix
• [Presentation] Integrated Pressure Driven Perfusion Culture Microchamber Array Chip For High-Throughput Drug Dose Resp onse Assay (2010)
  • Author(s): Shinji Sugiura, Koji Hattori, Masato Fujihashi, Masaya Kurokawa, Yuichi Nakano, Masumi Yanagisawa, Toshiyuki Kanamori
  • Organizer: The International Symposium on Microchemistry and Microsystems (ISMM) 2010
  • Place of Presentation: Hong Kong
• [Presentation] 集積化灌流培養マイクロチャンバーアレイチップによる薬剤濃度依存性試験の効率化 (2010)
  • Author(s): 服部浩二、杉浦慎治、藤橋政人、黒川正也、中野優一、柳澤真澄、金森敏幸
  • Organizer: 第21回 化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 東京
• [Presentation] マイクロプロセスを利用した細胞マニピュレーション/アッセイシステムの開発 (2010)
  • Author(s): 杉浦慎治、服部浩二、須丸公雄、金森敏幸
  • Organizer: 化学工学会 第42回秋季大会
  • Place of Presentation: 京都
• [Presentation] 灌流培養マイクロチャンバーアレイと段階希釈流路網を集積化した細胞アッセイチップの開発 (2010)
  • Author(s): 杉浦慎治、藤橋政人、黒川正也、中野優一、柳澤真澄、金森敏幸
  • Organizer: 第21回 化学とマイクロ・ナノシステム研究会
  • Place of Presentation: 名古屋
• [Presentation] 医薬品アッセイへの応用を目指した細胞チップ開発 (2010)
  • Author(s): 金森敏幸、杉浦慎治、服部浩二、須丸公雄
  • Organizer: 日本動物実験代替法学会第23回大会
  • Place of Presentation: 東京
• [Remarks]
  • URL: http://unit.aist.go.jp/scrc/ci/teams/drugassay/index.html
• [Patent(Industrial Property Rights)] 液体導入用デバイス (2011)
  • Inventor(s): 杉浦慎治、金森敏幸
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Acquisition Date: 2011-10-07
• [Patent(Industrial Property Rights)] マイクロチップ、マスターチップ (2007)
  • Inventor(s): 杉浦慎治、服部浩二、金森敏幸
  • Industrial Property Rights Holder: 独立行政法人産業技術総合研究所
  • Industrial Property Number: 2007-279711
  • Filing Date: 2007-10-26