Study on a novel meiosis specific chromosomal factor in vertebrate

• Project/Area Number: 22770001
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Genetics/Genome dynamics
• Research Institution: The University of Tokyo
• Principal Investigator: ISHIGURO Kei-ichiro 東京大学, 分子細胞生物学研究所, 助教 (30508114)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 減数分裂 / 染色体 / 生殖細胞 / コヒーシン / 染色体分配 / 細胞分裂

Research Abstract

We identified a novel cohesin subunit RAD21L which isspecifically expressed in meiotic prophase. The meiosis-specific cohesins REC8 and RAD21Lhave been shown to localize along axial elements in a mutually exclusive manner until pachytene inmammalian meiosis. We examined the processes of axial element (AE) formation by comparablyanalyzing Rec8KO, Rad21LKO and Rec8/Rad21L double KO. Disruption of both REC8 andRAD21L abolishes AE formation, suggesting that meiotic cohesins are essential for AE formation.

Research Products

• [Journal Article] Meiosis specific cohesins in mouse embryonic oocytes (2013)
  • Author(s): Kim J., Ishiguro K. , Kudo N., Watanabe Y
  • Journal Title: Methods in Molecular Biology Volume: 957 Pages: 47-57
  • Peer Reviewed
• [Journal Article] Studying Meiosis・Specific Cohesins in Mouse Embryonic Oocytes (2013)
  • Author(s): Kim J., et al.
  • Journal Title: Methods in Molecular Biology Volume: 957 Pages: 47-57
  • DOI: 10.1007/978-1-62703-191-2_3
  • ISBN: 9781627031905, 9781627031912
  • Peer Reviewed
• [Journal Article] A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. (2012)
  • Author(s): Morimoto A, Shibuya H, Zhu X, Kim J Ishiguro K, Han M, Watanabe Y.
  • Journal Title: J Cell Biol Volume: 198 Pages: 165-172
  • Peer Reviewed
• [Journal Article] A conserved KASH domain protein associates with telomeres, SUN1, and dynactin during mammalian meiosis. (2012)
  • Author(s): Morimoto A., et al.
  • Journal Title: Journal of Cell Biology Volume: 198(2) Issue: 2 Pages: 165-172
  • DOI: 10.1083/jcb.201204085
  • Peer Reviewed
• [Journal Article] A new meiosis-specific cohesin complex implicated in the cohesin code for homologous pairing (2011)
  • Author(s): Ishiguro K., Kim J., Fujiyama-Nakamura Kato S., Watanabe Y.
  • Journal Title: EMBO report Volume: 12 Pages: 267-275
  • Peer Reviewed
• [Journal Article] Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres (2010)
  • Author(s): Tanno Y., Kitajima T., Honda T., Ando Y.,Ishiguro K., Watanabe Y.
  • Journal Title: Genes&Development Volume: 24 Pages: 2169-2179
  • Peer Reviewed
• [Journal Article] Phosphorylation of mammalian Sgo2 by Aurora B recruits PP2A and MCAK to centromeres (2010)
  • Author(s): Tanno Y, et al.
  • Journal Title: Genes&Development. Volume: 24 Pages: 2169-2179
  • Peer Reviewed
• [Journal Article] Phosphorylation of H2A by Bub1 Prevents Chromosomal Instability Through Localizing Shugoshin (2010)
  • Author(s): Kawashima SA, et al.
  • Journal Title: Science Volume: 327 Pages: 172-177
  • Peer Reviewed
• [Presentation] Cohesin mediates DSB-independent homolog pairing in mouse meiosis (2012)
  • Author(s): Kei-ichiro Ishiguro, Jihye Kim,Abrahan Hernandez, Aussie Suzuki,Tatsuro Fukagawa, Christer Hoog, Yoshinori Watanabe.
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] PLK1 might regulate centromeric protection and mono-orientation at meiosis I in mouse oocyte (2012)
  • Author(s): Jihye Kim, Kei-ichiro Ishiguro, Watanabe Y.
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-13
• [Presentation] 減数分裂期特異的な新規テロメアタンパク質によるコヒーシンを介した染色体動態の制御 (2012)
  • Author(s): 澁谷大輝、石黒啓一郎、渡邊嘉典
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] 減数分裂特異的コヒーシン複合体の相同染色体のペアリング過程における役割 (2012)
  • Author(s): 石黒啓一郎、金智慧、鈴木應志、深川竜郎、渡邊嘉典
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 仙台
  • Year and Date: 2012-12-07
• [Presentation] The REC8- and RAD21L-cohesins promote the initial homolog pairing in mouse spermatocytes. (2012)
  • Author(s): Kei-ichiro Ishiguro, Jihye Kim, Abrahan Hernandez, Aussie Suzuki,Tatsuro Fukagawa Christer Hoog, Yoshinori Watanabe.
  • Organizer: Gordon Research Conference
  • Place of Presentation: New Hampshire, U.S.A
  • Year and Date: 2012-06-03
• [Presentation] The REC8- and RAD21L-cohesins promote the initial homolog pairing in mouse spermatocytes. (2012)
  • Author(s): Kei-ichiro Ishiguro
  • Organizer: Gordon Research Conference Meiosis
  • Place of Presentation: New Hampshire, U.S.A.
  • Invited
• [Presentation] Cohesin mediates DSB-independent homolog pairing in mouse meiosis (2012)
  • Author(s): Kei-ichiro Ishiguro
  • Organizer: 日本分子生物学会ワークショップ
  • Place of Presentation: 福岡県
  • Invited
• [Presentation] A novel meiosis specific MeiRad21L cohesin is implicated in "cohesin code" for chromosome dynamics in mammalian meiosis (2011)
  • Author(s): Kei-ichiro Ishiguro, Jihey Kim, Sally Fujiyama, Shigeaki Kato, Yoshinori Watanabe
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 小松
  • Year and Date: 2011-01-11
• [Presentation] A novel meiosis specific MeiRad21L cohesin is implicated in cohesin code for chromosome dynamics in mammalian meiosis (2011)
  • Author(s): 石黒啓一郎
  • Organizer: 染色体ワークショップ
  • Place of Presentation: 瑠璃光コンベンションホール(小松)
  • Year and Date: 2011-01-11
• [Presentation] Identification and characterization of a novel cohesin subunit in vertebrate meiosis (2010)
  • Author(s): Keiichiro Ishiguro, Jihey Kim, Sally Fujiyama, Shigeaki Kato, Yoshinori Watanabe
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Identification and characterization of a novel cohesin subunit in vertebrate meiosis (2010)
  • Author(s): 石黒啓一郎
  • Organizer: 日本分子生物学会
  • Place of Presentation: 神戸国際会議場(神戸)
  • Year and Date: 2010-12-10
• [Presentation] Identification and characterization of a novel cohesin subunit in vertebrate meiosis. (2010)
  • Author(s): Kei-ichiro Ishiguro, Jihye Kim,,Fujiyama-Nakamura S., Kato S.,Watanabe Y.
  • Organizer: ENS de Lyon-Workshop
  • Place of Presentation: Lyon, France
  • Year and Date: 2010-11-09