| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Research Abstract |
Sister chromatids, products of DNA replication, are held together by cohesin complex, which is vital for faithful chromosome segregation. When the cohesion is established, replication-dependent acetylation of a cohesin subunit Smc3 by Eco1 acetylase plays a critical role. In this study, I investigated how the cohesin acetylation is regulated in the cell cycle and how Eco1's function is coupled with DNA replication. Using an antibody specifically recognizing acetylated Smc3, I found the following.(1) Cohesin is deacetylated specifically by Hos1. This deacetylation is important in recycling cohesion complex for the next cell cycle.(2) Efficient acetylation of cohesin in S phase requires the presence of Ctf18-RFC, Ctf4 and Chl1, all of which are implicated in DNA replication. Subsequent analyses suggested that coordinated replication machinery action, particularly lagging strand synthesis, may play an important role in coupling of cohesin acetylation to DNA replication
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