| Project/Area Number |
22770008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Chubu University |
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Principal Investigator |
GOTO Yuji 中部大学, 生命健康科学部, 助手 (70362522)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ゲノム機能 / 発現 / X染色体 / 不活性化 / ヒストン修飾 / クロマチン構造 / 転写制御 / 不活性X染色体 / Matrix attachment region / KLHL15 / EIF2S3 |
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| Research Abstract |
In this study, I clarified the structure and mechanism of chromatin boundary between the inactivated KLHL15 and an escape gene, EIF2S3 by using human-mouse hybrid cells carrying either active or inactive human X chromosome in mouse background. Main findings are as follows;1) Three matrix attachment regions (MAR) located between KLHL15 and EIF2S3 in the inactive X-chromosome have been exclusively associated to nuclear matrix.2) Acetylation of histone H3K9 and methylation of histone H3K4 have been enriched at the escape gene, EIF2S3, and their flanking region. Tri-methylation of histone H3K27 and H3K9, on the other hand, have been enriched at the inactivated, KLHL15. These modifications were separated at MAR.3) Knockdown of SATB1, which is the member of MAR associated protein complex, led to the disruption of MAR and nuclear matrix pairing and histone modification.
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