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Chromatin structure and nuclear territories of the intergenic region between inactivated and escape genes on human inactive X chromosome.

Research Project

Project/Area Number 22770008
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Genetics/Genome dynamics
Research InstitutionChubu University

Principal Investigator

GOTO Yuji  中部大学, 生命健康科学部, 助手 (70362522)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsゲノム機能 / 発現 / X染色体 / 不活性化 / ヒストン修飾 / クロマチン構造 / 転写制御 / 不活性X染色体 / Matrix attachment region / KLHL15 / EIF2S3
Research Abstract

In this study, I clarified the structure and mechanism of chromatin boundary between the inactivated KLHL15 and an escape gene, EIF2S3 by using human-mouse hybrid cells carrying either active or inactive human X chromosome in mouse background. Main findings are as follows;1) Three matrix attachment regions (MAR) located between KLHL15 and EIF2S3 in the inactive X-chromosome have been exclusively associated to nuclear matrix.2) Acetylation of histone H3K9 and methylation of histone H3K4 have been enriched at the escape gene, EIF2S3, and their flanking region. Tri-methylation of histone H3K27 and H3K9, on the other hand, have been enriched at the inactivated, KLHL15. These modifications were separated at MAR.3) Knockdown of SATB1, which is the member of MAR associated protein complex, led to the disruption of MAR and nuclear matrix pairing and histone modification.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (23 results)

All 2013 2012 2011 2010

All Journal Article (13 results) (of which Peer Reviewed: 13 results) Presentation (10 results)

  • [Journal Article] c-Ret-mediated hearing losses.2012

    • Author(s)
      Ohgami N, Tamura H, Ohgami K, Iida M, Yajima I, Kumasaka MY, Goto Y, Sone M, Nakashima T, and Kato M.
    • Journal Title

      International Journal of Clinical and Experimental Pathology.

      Volume: 5 Pages: 23-8

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] RAS/RAF/MEK/ERK and PI3K/PTEN/AKT2012

    • Author(s)
      Yajima I, Kumasaka MY, Thang ND, Goto Y, Takeda K, Yamanoshita O, Iida M, Ohgami N, Tamura H, Kawamoto Y, and Kato M.
    • Journal Title

      Signaling in Malignant Melanoma Progression and Therapy.

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] c-Ret-mediated hearing losses.2012

    • Author(s)
      Ohgami N
    • Journal Title

      International Journal of Clinical and Experimental Pathology.

      Volume: 5 Pages: 23-28

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] RAS/RAF/MEK/ERK and PI3K/PTEN/AKT Signaling in Malignant Melanoma Progression and Therapy.2012

    • Author(s)
      Yajima I
    • Journal Title

      Dermatology Research and Practice.

      Volume: 2012 Pages: 354191-96

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Molecular network associated with MITF in skin melanoma development and progression.2011

    • Author(s)
      Yajima I, Kumasaka MY, Thang ND, Goto Y,Takeda K, Iida M, Ohgami N, Tamura H, Yamanoshita O, Kawamoto Y, Furukawa K, and Kato M.
    • Journal Title

      Journal of skin cancer.

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidative effects of cherry leaves extract on tert-butyl hydroperoxide-mediated cytotoxicity through regulation of thioredoxin-2 protein expression levels.2011

    • Author(s)
      Taguchi N, Uemura N, Goto Y, Sakura M, Hara K, Niwa M, Iida M, Yanagishita T,Watanabe D, and Kato M.
    • Journal Title

      J Toxicology and Environmental Health PartA.

      Volume: 74 Pages: 1240-1247

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Sunlight exposure-mediated DNA damage in young adults.2011

    • Author(s)
      Kato M, Iida M, Goto Y, Kondo T, andYajima I.
    • Journal Title

      Cancer Epidemiology and BiomarkersPreview.

      Volume: 20 Pages: 1622-1688

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] L-cysteine as a regulator for arsenic-mediated cancer-promoting and anti-cancer effects.2011

    • Author(s)
      Kato M, Kumasaka MY, Takeda K, HossainK, Iida M, Yajima I, Goto Y, and Ohgami N.
    • Journal Title

      Toxicology In Vitro.

      Volume: 25 Pages: 623-629

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Molecular Network Associated with MITF in Skin Melanoma Development and Progression.2011

    • Author(s)
      Ya manoshita O Kato M
    • Journal Title

      J Skin Cancer.

      Volume: 2011 Pages: 730170-730170

    • DOI

      10.1155/2011/730170

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidative effects of cherry leaves extract on tert-butyl hydroperocide-mediated cytotoxicity through regulation of thioredoxin-2 protein expression levels2011

    • Author(s)
      Taguchi N, Uemura N, Goto Y, Sakura M, Hara K, Niwa M, Iida M, Yanagishita T, Watanabe D, Kato M
    • Journal Title

      J Toxicol Environ Health A

      Volume: 74 Issue: 18 Pages: 1240-7

    • DOI

      10.1080/15287394.2011.570229

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Sunlight exposure-mediated DNA damage in young adults2011

    • Author(s)
      Kato M, Iida M, Goto Y, Kondo T, Yajima I
    • Journal Title

      Cancer Epidemiol Biomarkers Prev

      Volume: 20 Pages: 1622-8

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Antioxidative effects of cherry leaves extract on tert-butyl hydroperoxide-mediated cytotoxicity through regulation of thioredoxin-2protein expression level2011

    • Author(s)
      Taguchi N, Uemura N, Goto Y, Sakura M, Hara K, Niwa M, Iida M, Yanagishita T, Watanabe D, Kato M
    • Journal Title

      Journal of Toxicology and Environmental Health, Part A

      Volume: (in press)

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] L-cysteine as a regulator for arsenic-mediated cancer-promoting and anti-cancer effects2010

    • Author(s)
      Kato M, Kumasaka M, Takeda K, Hossain K, Iida M, Yajima I, Goto Y, Ohgami N
    • Journal Title

      Toxicology in Vitro

      Volume: 25 Pages: 623-629

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] 不活性化を免れる遺伝子と不活性化される遺伝子の境界領域のクロマチン構造2013

    • Author(s)
      後藤友二
    • Organizer
      哺乳動物X染色体研究会
    • Place of Presentation
      北大、札幌
    • Year and Date
      2013-03-23
    • Related Report
      2012 Final Research Report
  • [Presentation] 不活性化を免れる遺伝子と不活性化される遺伝子の境界領域のクロマチン構造2013

    • Author(s)
      後藤 友二
    • Organizer
      哺乳動物X染色体研究会
    • Place of Presentation
      北海道大学 札幌
    • Related Report
      2012 Annual Research Report
  • [Presentation] 不活性化を免れる遺伝子と不活性化される遺伝子の境界領域のクロマ チン構造と転写制御2012

    • Author(s)
      後藤友二
    • Organizer
      日本遺伝学会第84回大会
    • Place of Presentation
      九大、福岡
    • Year and Date
      2012-09-25
    • Related Report
      2012 Final Research Report
  • [Presentation] 不活性化を免れる遺伝子と不活性化される遺伝子の境界領域のクロマチン構造と転写制御2012

    • Author(s)
      後藤 友二
    • Organizer
      日本遺伝学会 第84回大会
    • Place of Presentation
      九州大学 福岡
    • Related Report
      2012 Annual Research Report
  • [Presentation] 不活性化を免れる遺伝子 EIF2S3 上流にあるクロマチン構造の解析 II2011

    • Author(s)
      後藤友二 、加藤昌志
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京大、京都
    • Year and Date
      2011-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 不活性化を免れる遺伝子EIF2S3上流にあるクロマチン構造の解析II2011

    • Author(s)
      後藤友二
    • Organizer
      日本遺伝学会第83回大会
    • Place of Presentation
      京都大学(京都)
    • Year and Date
      2011-09-20
    • Related Report
      2011 Annual Research Report
  • [Presentation] メラノーマ自然発症モデルマウスを用いた癌抑制遺伝子のスクリーニングと機能解析2010

    • Author(s)
      後藤友二 、加藤昌志
    • Organizer
      日本分子生物学会第33回大会
    • Place of Presentation
      ポートアイランド、神戸
    • Year and Date
      2010-12-08
    • Related Report
      2012 Final Research Report
  • [Presentation] メラノーマ自然発症モデルマウスを用いた癌抑制遺伝子のスクリーニングと機能解析2010

    • Author(s)
      後藤友二
    • Organizer
      第33回日本分子生物学会年会
    • Place of Presentation
      ポートアイランド(神戸)
    • Year and Date
      2010-12-08
    • Related Report
      2010 Annual Research Report
  • [Presentation] 不活性化を免れる遺伝子 EIF2S3 上流にあるクロマチン構造の解析2010

    • Author(s)
      後藤友二 、加藤昌志
    • Organizer
      日本遺伝学会第82回大会
    • Place of Presentation
      北大、札幌
    • Year and Date
      2010-09-20
    • Related Report
      2012 Final Research Report
  • [Presentation] 不活性化を免れる遺伝子EIF2S3上流にあるクロマチン構造の解析2010

    • Author(s)
      後藤友二
    • Organizer
      日本遺伝学会 第82回大会
    • Place of Presentation
      北海道大学(札幌)
    • Year and Date
      2010-09-20
    • Related Report
      2010 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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