Chromatin structure and nuclear territories of the intergenic region between inactivated and escape genes on human inactive X chromosome.
| Project/Area Number |
22770008
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Chubu University |
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Principal Investigator |
GOTO Yuji 中部大学, 生命健康科学部, 助手 (70362522)
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | ゲノム機能 / 発現 / X染色体 / 不活性化 / ヒストン修飾 / クロマチン構造 / 転写制御 / 不活性X染色体 / Matrix attachment region / KLHL15 / EIF2S3 |
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| Research Abstract |
In this study, I clarified the structure and mechanism of chromatin boundary between the inactivated KLHL15 and an escape gene, EIF2S3 by using human-mouse hybrid cells carrying either active or inactive human X chromosome in mouse background. Main findings are as follows;1) Three matrix attachment regions (MAR) located between KLHL15 and EIF2S3 in the inactive X-chromosome have been exclusively associated to nuclear matrix.2) Acetylation of histone H3K9 and methylation of histone H3K4 have been enriched at the escape gene, EIF2S3, and their flanking region. Tri-methylation of histone H3K27 and H3K9, on the other hand, have been enriched at the inactivated, KLHL15. These modifications were separated at MAR.3) Knockdown of SATB1, which is the member of MAR associated protein complex, led to the disruption of MAR and nuclear matrix pairing and histone modification.
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Report
(4 results)
Research Products
(23 results)
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[Journal Article] c-Ret-mediated hearing losses.2012
Author(s)
Ohgami N, Tamura H, Ohgami K, Iida M, Yajima I, Kumasaka MY, Goto Y, Sone M, Nakashima T, and Kato M.
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Journal Title
International Journal of Clinical and Experimental Pathology.
Volume: 5
Pages: 23-8
Related Report
Peer Reviewed
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[Journal Article] RAS/RAF/MEK/ERK and PI3K/PTEN/AKT2012
Author(s)
Yajima I, Kumasaka MY, Thang ND, Goto Y, Takeda K, Yamanoshita O, Iida M, Ohgami N, Tamura H, Kawamoto Y, and Kato M.
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Journal Title
Signaling in Malignant Melanoma Progression and Therapy.
Related Report
Peer Reviewed
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[Journal Article] Molecular network associated with MITF in skin melanoma development and progression.2011
Author(s)
Yajima I, Kumasaka MY, Thang ND, Goto Y,Takeda K, Iida M, Ohgami N, Tamura H, Yamanoshita O, Kawamoto Y, Furukawa K, and Kato M.
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Journal Title
Related Report
Peer Reviewed
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[Journal Article] Antioxidative effects of cherry leaves extract on tert-butyl hydroperoxide-mediated cytotoxicity through regulation of thioredoxin-2protein expression level2011
Author(s)
Taguchi N, Uemura N, Goto Y, Sakura M, Hara K, Niwa M, Iida M, Yanagishita T, Watanabe D, Kato M
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Journal Title
Journal of Toxicology and Environmental Health, Part A
Volume: (in press)
Related Report
Peer Reviewed
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