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Analysis of the X chromosome inactivation-reactivation cycle throughout the mouse life cycle

Research Project

Project/Area Number 22770010
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Genetics/Genome dynamics
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

SUGIMOTO Michihiko  独立行政法人理化学研究所, 動物変異動態解析技術開発チーム, 開発研究員 (10373317)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
KeywordsX染色体不活性化 / マウス / 初期胚発生 / エピジェネティクス / 初期発生 / DNAメチル化 / 始原生殖細胞
Research Abstract

It is known that the X chromosome of female mammals shows inactivation-reactivation cycles throughout the life cycle, especially at the peri-implantation stage, the changes of the X chromosome activity occur during short period. In this study, it was indicated that the re-inactivation of the X chromosome in the epiblast, the future embryo proper, occurs just after implantation. Furthermore, the dynamics of the DNA methylation of the inactive X linked genes during the X reactivation in primordial germ cells was elucidated.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (12 results)

All 2011 2010 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (6 results) Remarks (2 results)

  • [Journal Article] RNAi mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos2011

    • Author(s)
      Matoba S., Inoue K., Kohda T., Sugimoto M., Mizutani E., Ogonuki N., Nakamura T., Abe K., Nakano T., Ishino F. and Ogura A.
    • Journal Title

      Proc. Natl. Acad. Sci. USA

      Volume: 108(51) Pages: 20621-20626

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] RNAi-mediated knockdown of Xist can rescue the impaired postimplantation development of cloned mouse embryos2011

    • Author(s)
      Matoba S, et al
    • Journal Title

      Proc Natl Acad Sci USA

      Volume: 108 Issue: 51 Pages: 20621-20626

    • DOI

      10.1073/pnas.1112664108

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer2010

    • Author(s)
      Inoue K., Kohda T., Sugimoto M., Sado T., Ogonuki N., Matoba S., Shiura H., Ikeda R., Mochida K., Fujii T., Sawai K., Otte A. P., Tian C., Yang X., Ishino F., Abe K. and Ogura A.
    • Journal Title

      Science

      Volume: 330 Pages: 496-499

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impeding Xist expression from the active X chromosome improves mouse somatic cell nuclear transfer.2010

    • Author(s)
      Inoue K., et al.
    • Journal Title

      Science

      Volume: 330 Pages: 496-499

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] t^<w5>劣性致死変異の責任遺伝子Vps52の機能解析/Functional analysis of Vps52, a responsible gene for the t^<w5> recessive lethal mutation2011

    • Author(s)
      杉本道彦、近藤昌代、廣瀬美智子、小倉淳郎、阿部訓也
    • Organizer
      日本遺伝学会83回大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] 特異な進化履歴を持つマウスt-コンプレックスのゲノム構造解析/Unexplored mouse genome : structure and evolution of the t-complex2011

    • Author(s)
      阿部訓也、沼田興治、池田理恵子、杉本道彦、太田聡史
    • Organizer
      日本遺伝学会83回大会
    • Place of Presentation
      京都
    • Related Report
      2011 Final Research Report
  • [Presentation] Dynamics of epigenomic changes during development of early embryos and primordial germ cells in mice2011

    • Author(s)
      Kuniya Abe, Koji Numata, Rieko Ikeda, Hirosuke Shiura and Michihiko Sugimoto
    • Organizer
      EMBO Workshop "50 Years of X-inactivation Research"
    • Place of Presentation
      Oxford
    • Related Report
      2011 Final Research Report
  • [Presentation] Dynamics of epigenomic changes during development of early embryos and primordial germ cells in mice2011

    • Author(s)
      Kuniya Abe, Koji Numata, Rieko Ikeda, Hirosuke Shiura, Michihiko Sugimoto
    • Organizer
      EMBO workshop "50 Years of X-inactivation Research"
    • Place of Presentation
      Oxford, UK
    • Related Report
      2011 Annual Research Report
  • [Presentation] Gene expression in embryonic pluripotential cells and germ cells in mice2010

    • Author(s)
      Abe K., et al.
    • Organizer
      International Symposium on "Epigenome Network, Development and Reprogramming of Germ Cells"
    • Place of Presentation
      Fukuoka, Japan
    • Year and Date
      2010-11-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] マウスt-complex劣性致死変異の同定により見えてきたVps52の機能-哺乳類初期発生における多能性細胞の分化制御因子としての役割-2010

    • Author(s)
      杉本道彦、阿部訓也
    • Organizer
      日本遺伝学会82回大会ワークショップ「マウス・ラットをモデルとした哺乳類遺伝学」
    • Place of Presentation
      札幌(招待講演)
    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.jp/lab/intro_heni.html

    • Related Report
      2011 Final Research Report
  • [Remarks]

    • URL

      http://www.brc.riken.jp/lab/mcd/mcd2/index.html

    • Related Report
      2011 Annual Research Report

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Published: 2010-08-23   Modified: 2016-04-21  

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