A metabolic pathway for generation of GDP-glucose in higher plants

• Project/Area Number: 22770030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Saitama University
• Principal Investigator: KOTAKE Toshihisa 埼玉大学, 大学院・理工学研究科, 准教授 (20334146)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 細胞壁 / 糖ヌクレオチド / グルコマンナン / GDP-マンノース / GDP-グルコース / 多糖類 / アスコルビン酸 / 植物 / シロイヌナズナ / ピロホスホリラーゼ

Research Abstract

The konjac(kjc)1 mutation appeared to affect glucomannan content. Recombinant KJC1 and KJC2 expressed in E. coli did not have any enzymatic activity, but they stimulated GDP-mannose/GDP-glucose synthase activity of VTC1. The results suggest that KJC1 and 2 are involved in glucomannan synthesis through the regulation of the synthesis of GDP-mannose and GDP-glucose catalyzed by VTC1.

Research Products

• [Journal Article] β-Galactosyl Yariv reagent binds to the β-1,3-galactan of arabinogalactan-proteins (2013)
  • Author(s): Kitazawa K., Tryfona T., Yoshimi Y., Hayashi Y., Kawauchi S., Antonov L., Tanaka H., Takahashi T., Kaneko S., Dupree P., Tsumuraya Y. and Kotake T
  • Journal Title: Plant Physiol. Volume: 161 Issue: 3 Pages: 1117-1126
  • DOI: 10.1104/pp.112.211722
  • Peer Reviewed
• [Journal Article] Structural characterisation of Arabidopsis leaf arabinogalactan polysaccharides (2012)
  • Author(s): Tryfona T., Liang H.-C., Kotake T., Tsumuraya Y., Stephens E. and Dupree P
  • Journal Title: Plant Physiol Volume: 160 Issue: 2 Pages: 653-666
  • DOI: 10.1104/pp.112.202309
  • Peer Reviewed
• [Journal Article] Rice Brittle culm 6 encodes a dominant negative form of CesA protein that perturbs cellulose synthesis in secondary cell walls (2011)
  • Author(s): Kotake T., Aohara T., Hirano K., Sato A., Kaneko Y., Tsumuraya Y., Takatsuji H., Kawasaki S.
  • Journal Title: Journal of Experimental Botany Volume: 62 Pages: 2053-2062
  • Peer Reviewed
• [Journal Article] Generation of nucleotide sugars for biomass formation in plants (2010)
  • Author(s): Kotake T., Hirosawa C., Ando Y. and Tsumuraya Y
  • Journal Title: Plant Biotechnol Volume: 27 Pages: 231-236
  • NAID: 10026527467
  • Peer Reviewed
• [Journal Article] Generation of nucleotide sugars for biomass formation in plants (2010)
  • Author(s): Kotake T., Hirosawa C., Ando Y., Tsumuraya Y.
  • Journal Title: Plant Biotechnology Volume: 27 Pages: 231-236
  • NAID: 10026527467
  • Peer Reviewed
• [Journal Article] 植物生体内で単糖類はどのようにリサイクルされるか? (2010)
  • Author(s): 小竹敬久、円谷陽一
  • Journal Title: 化学と生物 Volume: 48 Pages: 402-408
  • NAID: 10026438581
• [Presentation] KONJAC1 and 2 Are Genes Involved in Glucomannan and L-Ascorbate Synthesis in Arabidopsis (2012)
  • Author(s): Toshihisa Kotake, Noriaki Tajima, Jenny Mortimer, Shota Sawake, Xiaolan Yu, Paul Dupree, and Yoichi Tsumuraya
  • Organizer: International Conference for Arabidopsis Research
  • Place of Presentation: オーストリア・ウィーン
  • Year and Date: 2012-07-03
• [Presentation] 細胞壁グルコマンナン合成におけるKONJAC1, 2の役割 (2012)
  • Author(s): 小竹敬久、Jenny Mortimer, 田島範明、宮崎祐一、Xiaolan Yu, Paul Dupree, 円谷陽一
  • Organizer: 日本植物生理学会
  • Place of Presentation: 京都府京都市・京都産業大学
  • Year and Date: 2012-03-17
• [Presentation] 植物の単糖再利用機構 (2011)
  • Author(s): 小竹敬久
  • Organizer: 日本生化学会シンポジウム
  • Place of Presentation: 京都府京都市(京都国際会館)
  • Year and Date: 2011-11-24
• [Presentation] KONJAC1 and 2 are genes involved in glucomannan and vitamin C synthesis in Arabidopsis (2011)
  • Author(s): Toshihisa Kotake, Noriaki Tajima, Yuichi Miyazaki, Jenny Mortimer, Xiaolan Yu, Paul Dupree, Yoichi Tsumuraya
  • Organizer: The 4th Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: 兵庫県淡路市・淡路夢舞台
  • Year and Date: 2011-10-06
• [Presentation] KONJAC1 and 2 Are Genes Involved in Glucomannan and Vitamin C Synthesis in Arabidopsis (2011)
  • Author(s): T.Kotake, N.Tajima, Y.Miyazaki, J.Mortimer, X.-Lan Yu, P.Dupree, Y.Tsumuraya
  • Organizer: Fourth Conference on Biosynthesis of Plant Cell Wall
  • Place of Presentation: 兵庫県淡路市(淡路夢舞台国際会議場)
  • Year and Date: 2011-10-06
• [Presentation] KONJAC1 and 2 genes encoding nucleotide sugar pyrophosphorylase are necessary for glucomannan synthesis in Arabidopsis (2010)
  • Author(s): Kotake T., Tajima N., Miyazaki Y., Mortimer J., Dupree P., Tsumuraya Y.
  • Organizer: Cell wall meeting
  • Place of Presentation: ポルト市(ポルトガル)
  • Year and Date: 2010-07-25
• [Book] 植物細胞壁(西谷和彦・梅澤俊明編) (2013)
  • Author(s): 小竹敬久(部分執筆)
  • Publisher: 講談社
• [Book] 植物細胞壁 (2013)
  • Author(s): 西谷和彦、梅澤俊明 編著
  • Total Pages: 349
  • Publisher: 講談社