Identification of the gene coding tyrosine hydroxylase using the transposon-tagging methods.

• Project/Area Number: 22770033
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Tokyo University of Agriculture and Technology
• Principal Investigator: SASAKI Nobuhiro 東京農工大学, 大学院・工学研究院, 助教 (80422088)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 代謝生理 / 植物色素 / ベタレイン

Research Abstract

The genomic region including promoter of the DOPA dioxygense(DOD) gene was isolated from Mirabilis jalapa. DNA sequence analysis revealed that the DOD gene was consisted of 3 exons and 2 introns. We, however, could not find any transposable element-like sequences in the genomic region. Three candidate cDNAs encoding tyrosine hydroxylase were isolated from petals of Mirabilis jalapa using cDNA subtraction method.

Research Products

• [Journal Article] In vitro synthesis of betaxanthins using recombinant DOPA 4, 5-dioxygenase and evaluation of their radical-scavenging activities (2010)
  • Author(s): Sekiguchi H, Ozeki Y, Sasaki N
  • Journal Title: J. Agric. Food Chem Volume: 58 Pages: 12504-12509
  • Peer Reviewed
• [Journal Article] 植物色素の排他性-アントシアニン色素とベタレイン色素 (2010)
  • Author(s): 佐々木伸大
  • Journal Title: 生物科学 Volume: 62 Pages: 30-38
• [Journal Article] In vitro synthesis of betaxanthins using recombinant DOPA 4,5-dioxygenase and evaluation of their radical-scavenging activities. (2010)
  • Author(s): 関口拓史(佐々木伸大)
  • Journal Title: Journal Agricultural and Food Chemistry Volume: 58 Pages: 12504-12509
  • Peer Reviewed
• [Presentation] 植物色素ベタキサンチンの酵素学的半合成 (2010)
  • Author(s): 関口拓史、佐々木伸大、小関良宏
  • Organizer: 第28回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 仙台
  • Year and Date: 2010-09-03
• [Presentation] 植物色素ベタキサンチンの酵素学的半合成 (2010)
  • Author(s): 関口拓史
  • Organizer: 第28回日本植物細胞分子生物学会
  • Place of Presentation: 東北大学(仙台)
  • Year and Date: 2010-09-03
• [Patent(Industrial Property Rights)] L-DOPAを蓄積する植物細胞及びその利用 (2010)
  • Inventor(s): 中塚貴司,高橋秀行,山田恵理,坂本裕一,関口拓史,佐々木伸大,小関良宏,西原昌宏
  • Industrial Property Rights Holder: 中塚貴司,高橋秀行,山田恵理,坂本裕一,関口拓史,佐々木伸大,小関良宏,西原昌宏
  • Filing Date: 2010-09-07