| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Research Abstract |
We analyzed the molecular mechanisms of the regulatory systems of three kinds ofnitrogen transporters (LtnT, CynABD, FocA) in cyanobacteria. We identified a novel protein (LtnE) that is involved in the activation of the LtnT transporter in a freshwater cyanobacterium. We clarified that the PII protein, which was a regulatory protein of various nitrogen assimilation proteins, was involved in the regulation of the activity of the CynABD transporter. We clarified that the C-terminal region of the FocA transporter inhibited the transport activity of the transporter and the FocA transport activity could be inactivated in the presence of ammonium in the medium. In addition, we established the culture method of a marine cyanobacterium and clarified that there was an unknownnitrite transport mechanism in the marine cyanobacterium.
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