Study on auxin-mediated cell cycle regulation

• Project/Area Number: 22770040
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: OKUSHIMA Yoko 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (00432592)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2010: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
• Keywords: 細胞周期 / サイクリン依存性キナーゼ / 植物 / シロイヌナズナ / 細胞分裂

Research Abstract

In this study, expression analyses of cell cycle regulators were performed to elucidate the mechanisms underlying auxin-mediated cell cycle regulation. We found that the level of B2-type cyclin-dependent kinase (CDKB2), a major regulator of plant cell cycle progression, is tightly controlled by multiple pathways to maintain the meristem size in developing roots.

Research Products

• [Journal Article] Synthesis of very-long-chain Fatty acids in the epidermis controls plant organ growth by restricting cell proliferation (2013)
  • Author(s): Nobusawa T, Okushima Y, Nagata N, Kojima M, Sakakibara H, Umeda M
  • Journal Title: PLoS Biol
  • NAID: 120005716835
  • Peer Reviewed
• [Journal Article] Kip-related protein 3 is required for control of endoreduplication in the shoot apical meristem and leaves of Arabidopsis (2013)
  • Author(s): Jun SE, Okushima Y, Nam J, Umeda M, Kim GT
  • Journal Title: Mol Cells Pages: 47-53
  • Peer Reviewed
• [Journal Article] Kip-related protein 3 is required for control of endoreduplication in the shoot apical meristem and leaves of Arabidopsis. (2013)
  • Author(s): Jun SE
  • Journal Title: Mol Cells Volume: 35 Pages: 47-53
  • Peer Reviewed
• [Journal Article] Synthesis of very-long-chain Fatty acids in the epidermis controls plant organ growth by restricting cell proliferation. (2013)
  • Author(s): Nobusawa T
  • Journal Title: PLoS Biol. Volume: 11
  • Peer Reviewed
• [Journal Article] Programmed induction of endoreduplication by DNA double-strand breaks in Arabidopsis (2011)
  • Author(s): Adachi, S., Minamisawa, K., Okushima, Y., Inagaki, S., Yoshiyama, K., Kondou, Y., Kaminuma, E., Kawashima, M., Toyoda, T., Matsui, M., Kurihara, D., Matsunaga, S., Umeda, M
  • Journal Title: Proc Natl Acad Sci U S A Volume: 108 Pages: 10004-10009
  • NAID: 120005716659
  • Peer Reviewed
• [Journal Article] A high concentration of nitrate causes temporal inhibition of lateral root growth by suppressing cell proliferation (2011)
  • Author(s): Okushima, Y., Inamoto, I., and Umeda, M
  • Journal Title: Plant Biotechnology Volume: 28 Pages: 413-416
  • NAID: 10029707439
  • Peer Reviewed
• [Presentation] Regulation of cytokinin biosynthesis genes by synthesis of very-long-chain fatty acids in Arabidopsis (2013)
  • Author(s): Yoko Okushima、 Megumi Ikeda、 Ayaka Yamasaki 、 Takashi Nobusawa and Masaaki Umeda
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] シロイヌナズナ CDKB2 の発現制御機構の解析 (2013)
  • Author(s): 奥島 葉子、清水 皓平、 梅田 正明
  • Organizer: 第30回日本植物分子生物学会大会
  • Place of Presentation: 生駒
• [Presentation] Umeda M Regulation of B-type CDK accumulation during Arabidopsis root development (2011)
  • Author(s): Okushima Y, Simizu K
  • Organizer: The International Symposium of "Strategies of Plants against Global Environmental Change"
  • Place of Presentation: 倉敷
• [Presentation] Regulation of B-type CDK accumulation during Arabidopsis root development (2011)
  • Author(s): Okushima Y, Simizu K, Umeda M
  • Organizer: Strategies of Plants against Global Environmental Change
  • Place of Presentation: 倉敷市(複数日ポスタ発表)
• [Presentation] Regulation of B-type CDK accumulation during Arabidopsis root development (2010)
  • Author(s): Okushima Y, Simizu K, Umeda M
  • Organizer: 第33回日本分子生物学会年会/BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] Synthesis of very long chain fatty acids in the epidermis suppresses overproliferation of cells in Arabidopsis shoot apices (2010)
  • Author(s): Nobusawa T, Okushima Y, Umeda M
  • Organizer: 第33回日本分子生物学会年会/BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] 超長鎖脂肪酸を介した植物の形態形成機構 (2010)
  • Author(s): 信澤 岳、奥島 葉子、梅田 正明
  • Organizer: 第23回植物脂質シンポジウム
  • Place of Presentation: 宇治
• [Presentation] シロイヌナズナCDKB2の発現制御機構の解析
  • Author(s): 奥島 葉子、清水 皓平、 梅田 正明
  • Organizer: 第30回日本植物細胞分子生物学会大会・シンポジウム
  • Place of Presentation: 奈良県生駒市
• [Presentation] Regulation of cytokinin biosynthesis genes by synthesis of very-long-chain fatty acids in Arabidopsis
  • Author(s): Yoko Okushima、Megumi Ikeda、Ayaka Ymasaki、Takashi Nobusawa、Masaaki Umeda
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山大学
• [Book] 植物の細胞周期制御(「植物のシグナル伝達 分子と応答」中) (2010)
  • Author(s): 奥島葉子、梅田正明
  • Publisher: 共立出版
• [Book] 植物の細胞周期制御(植物のシグナル伝達 分子と応答 中) (2010)
  • Author(s): 奥島葉子
  • Publisher: 共立出版