Elucidation of chloroplast-mediated calcium signaling in] Arabidopsis mesophyll cells

• Project/Area Number: 22770046
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Osaka Medical College
• Principal Investigator: HARADA Akiko 大阪医科大学, 医学部, 講師 (80360626)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2010: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
• Keywords: シロイヌナズナ / 光合成 / 葉緑体 / カルシウムシグナル / 赤色光 / カルシウムイオン

Research Abstract

We focused on a role of calcium signal in the chloroplast-mediated signaling pathway. We revealed that red light increased cytosolic Ca2+ through operation of photosynthesis in Arabidopsis leaves.

Research Products

• [Journal Article] Role of RPT2 in leaf positioning and flattening and a possible inhibition of phot2 signaling by phot1. (2013)
  • Author(s): Akiko Harada, Atsushi Takemiya, Shin-ichiro Inoue, Tatsuya Sakai, Ken-ichiro Shimazaki
  • Journal Title: Plant and Cell Physiology Volume: Vol. 54、 Pages: 36-47
  • NAID: 10031145648
  • Peer Reviewed
• [Journal Article] Disruption of ROOT PHOTOTROPISM2 gene does not affect phototropin-mediated stomatal opening (2013)
  • Author(s): Tsutsumi T, Takemiya A, Harada A and Shimazaki K
  • Journal Title: Plant Science Volume: 201 Pages: 93-97
  • DOI: 10.1016/j.plantsci.2012.11.012
  • Peer Reviewed
• [Journal Article] Role of RPT2 in leaf positioning and flattening and a possible inhibition of phot2 signaling by phot1 (2013)
  • Author(s): Harada A, Takemiya A, Inoue S, Sakai T and Shimazaki K
  • Journal Title: Plant Cell Physiol. Volume: 54 Issue: 1 Pages: 36-47
  • DOI: 10.1093/pcp/pcs094
  • NAID: 10031145648
  • Peer Reviewed
• [Journal Article] Arabidopsis molybdopterin biosynthesis protein Cnx5 collaborates with the ubiquitin-like protein Urm11 in the thio-modification of tRNA (2012)
  • Author(s): Yumi Nakai, Akiko Harada, Yasuyuki Hashiguchi, Masato Nakai, Hideyuki Hayashi
  • Journal Title: Journal of Biological Chemistry Volume: Vol. 287 Issue: 36 Pages: 30874-30884
  • DOI: 10.1074/jbc.m112.350090
  • Peer Reviewed
• [Journal Article] The WAVY GROWTH 3 E3 ligase family controls the gravitropic response in Arabidopsis roots. (2012)
  • Author(s): Tatsuya Sakai, et al.
  • Journal Title: Plant J. Volume: 70 Issue: 2 Pages: 303-314
  • DOI: 10.1111/j.1365-313x.2011.04870.x
  • Peer Reviewed
• [Journal Article] Changes in Ca2+ metabolismin Arabidopsis guard cells in response to blue light. (2010)
  • Author(s): Akiko Harada (2010)
  • Journal Title: Plant Signaling and Behavior Volume: Vol. 5 Pages: 397-400
• [Journal Article] Changes in Ca^<2+> metabolism in Arabidopsis guard cells in response to blue light (2010)
  • Author(s): Akiko Harada
  • Journal Title: Plant Signaling and Behavior Volume: 5 Pages: 397-400
• [Journal Article] Biochemical characterization of Calcineulin B-like interacting protein kinase in Vicia guard cells (2010)
  • Author(s): Misumi Tominaga, Akiko Harada, Toshinori Kinoshita, Ken-ichiro Shimazaki
  • Journal Title: Plant and Cell Physiology Volume: 51 Pages: 408-421
  • Peer Reviewed
• [Presentation] RPT2 は葉の定位と平 滑化に関与している (2013)
  • Author(s): 原田明子、武宮淳史、井上晋一郎、酒井 達也、島崎研一郎
  • Organizer: 第54回 日本植物 生理学会年会
  • Place of Presentation: 岡山
• [Presentation] AtCnx5, Arabidopsis sulfurtransferase protein involved in molybdopterin biosynthesis, is also required to the thio-modification of tRNAs. (2013)
  • Author(s): Yumi Nakai, Akiko Harada, Yasuyuki Hashiguchi, Masato Nakai
  • Organizer: 第54回 日本植物生理学会年会・
  • Place of Presentation: 岡山
• [Presentation] RPT2は葉の定位と平滑化に関与している (2013)
  • Author(s): 原田明子
  • Organizer: 第54回 日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] AtCnx5, Arabidopsis sulfurtransferase protein involved in molybdopterin biosynthesis, is also required to the thio-modification of tRNAs. (2013)
  • Author(s): Yumi Nakai
  • Organizer: 第54回 日本植物生理学会年会
  • Place of Presentation: 岡山
• [Presentation] 光応答とカルシウムシグナ ル (2012)
  • Author(s): 原田明子
  • Organizer: 第11回 植物生体膜談シンポジウム植物細胞におけるカルシウムシグナ リング
  • Place of Presentation: 姫路
  • Year and Date: 2012-09-15
• [Presentation] Urm1 と Uba4 は細胞内含 硫小分子への硫黄運搬と Urmylation に 関わる UBL-UBA システムである (2012)
  • Author(s): 中井由実, 原田明子, 橋口康之, 中井 正人, 林 秀行
  • Organizer: 第85回日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] 光応答とカルシウムシグナル (2012)
  • Author(s): 原田明子
  • Organizer: 第11回 植物生体膜談シンポジウム
  • Place of Presentation: 姫路
  • Invited
• [Presentation] Urm1とUba4は細胞内含硫小分子への硫黄運搬とUrmylationに関わるUBL-UBAシステムである (2012)
  • Author(s): 中井由実
  • Organizer: 第85回 日本生化学会大会
  • Place of Presentation: 福岡
• [Presentation] シロイヌナズナおよび酵母においてサイトゾルのtRNA硫黄修飾に関与する、ユビキチン様(UBI)タンパク質とユビキチン活性化酵素様(UBA)タンパク質の比較解析 (2011)
  • Author(s): 中井由実、原田明子、橋口康之、中井正人、林秀行
  • Organizer: 第84回生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-20
• [Presentation] Proteins required for thio-modification of tRNAs participate in the biosynthesis of surfur-containing cofactors (2011)
  • Author(s): Yumi Nakai, Akiko Harada, Yasuyuki Hashiguchi, Takeshi Murakawa, Masato Nakai, Hideyuki Hayashi
  • Organizer: The Third International Conference on Cofactors 03 (ICC-03)
  • Place of Presentation: トゥルク
  • Year and Date: 2011-07-12
• [Presentation] シロイヌナズナ孔辺細胞青色光情報伝達におけるRPT2の機能解析 (2011)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一郎
  • Organizer: 第52回 日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-21
• [Presentation] シロイヌナズナおよび酵母 においてサイトゾルの tRNA 硫黄修飾に 関与する、ユビキチン様(UBI)タンパ ク質とユビキチン活性化酵素様(UBA) タンパク質の比較解析 (2011)
  • Author(s): 中井由実、原田明子、橋口康之、中井正 人、林秀行
  • Organizer: 第84回生化学 会大会・
  • Place of Presentation: 京都
• [Presentation] シロイヌナズナ孔辺細胞青色光情報 伝達における RPT2 の機能解析 (2011)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一 郎
  • Organizer: 第52回 日本植物生理学会年会
  • Place of Presentation: 仙台
• [Presentation] 孔辺細胞青色光情報伝達における RPT2 の機能解析 (2010)
  • Author(s): 堤俊文、武宮淳史、原田明子、島崎研一 郎
  • Organizer: 第55回三学会合同大 会植物学会 九州・沖縄支部会
  • Place of Presentation: 福岡
• [Remarks] 大阪医科大学生物学教室
  • URL: http://www.osaka-med.ac.jp/deps/bio/biology006.htm
• [Remarks]
  • URL: http://www.osaka-med.ac.jp/deps/bio/biology006.htm
• [Remarks]
  • URL: http://www.osaka-med.ac.jp/deps/bio/biology006.htm