The activation mechanism of field resistance gene, Pb1

• Project/Area Number: 22770048
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: INOUE Haruhiko 独立行政法人農業生物資源研究所, 耐病性作物研究開発ユニット, 主任研究員 (10435612)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
• Keywords: 抵抗性遺伝子 / いもち病 / 転写因子 / 圃場抵抗性 / プロテアソーム分解 / イネ / マイクロアレイ / シグナル伝達

Research Abstract

Panicle blast1 (Pb1) is useful gene for rice breeding, and its protein structure is similar to that of true resistance proteins. In this study, we identified that Pb1 directly interacts with WRKY45 transcription factor and the interaction is required for the resistance. The interaction stabilizes WRKY45 protein, and enhances the defense activation.

Research Products

• [Journal Article] Nuclear ubiquitin proteasome degradation affects WRKY45 function in the rice defense program (2013)
  • Author(s): Matsushita A, Inoue H, Goto S, Nakayama A, Sugano S, Hayashi N, Takatsuji H
  • Journal Title: The Plant Journal Volume: 73(2) Pages: 302-313
  • Peer Reviewed
• [Journal Article] Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice (2013)
  • Author(s): Jiang C-J, Shimono M, Sugano S, Kojima M, Liu X, Inoue H, Sakakibara H, Takatsuji H
  • Journal Title: Molecular Plant-Microbe Interactions Volume: 26(3) Pages: 287-296
  • Peer Reviewed
• [Journal Article] Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein-protein interaction (2013)
  • Author(s): Inoue H, Hayashi N, Matsushita A, Xinqiong L, Nakayama A, Sugano S, Jiang C-J, Takatsuji H
  • Journal Title: Proceedings of the National Academy of Sciences of the United States of America Volume: 110(23) Pages: 9577-9582
  • Peer Reviewed
• [Journal Article] Blast resistance of CC-NB-LRR protein Pb1 is mediated by WRKY45 through protein–protein interaction (2013)
  • Author(s): Inoue H
  • Journal Title: Proceedings of the National Academy of Sciences Volume: 未定
  • Peer Reviewed
• [Journal Article] Nuclear ubiquitin proteasome degradation affects WRKY45 function in the rice defense program (2013)
  • Author(s): Matsushita A
  • Journal Title: The Plant Journal Volume: 73 Issue: 2 Pages: 302-313
  • DOI: 10.1111/tpj.12035
  • Peer Reviewed
• [Journal Article] Rice WRKY45 plays important roles in fungal and bacterial disease resistance (2012)
  • Author(s): Shimono M, Koga H, Akagi A, Hayashi N, Goto S, Sawada M, Kurihara T, Matsushita A, Sugano S, Jiang C-J, Kaku H, Inoue H, Takatsuji H
  • Journal Title: Molecular Plant Pathology Volume: 13(1) Pages: 83-94
  • Peer Reviewed
• [Journal Article] Durable panicle blast-resistance gene Pb1 encodes an atypical CC-NBS-LRR protein and was generated by acquiring a promoter through local genome duplication (2010)
  • Author(s): Hayashi, Inoue, et al
  • Journal Title: The plant journal Volume: 64 Pages: 498-510
  • Peer Reviewed
• [Presentation] いもち病圃場抵抗性遺伝子Pb1の抵抗性はWRKY45を介している (2012)
  • Author(s): 井上晴彦
  • Organizer: 第53回日本植物生理学会
  • Place of Presentation: 京都産業大学(京都府)
  • Year and Date: 2012-03-16
• [Presentation] いもち病圃場抵抗性遺伝子Pb1の抵抗性はWRKY45を介している (2012)
  • Author(s): 井上晴彦, 林長生, 松下茜, 中山明, 菅野正治, 姜昌杰, 高辻博志
  • Organizer: 第53回日本植物生理学会年会講演
• [Presentation] イネのWRKY45の機能発現はユビキチン/プロテアソームにより2つのモードで制御されている (2012)
  • Author(s): 松下茜, 井上晴彦, 後藤新悟, 中山明, 菅野正治, 高辻博志
  • Organizer: 第53回日本植物生理学会年会講演
• [Presentation] Inoue H, Hayashi N, Matsushita A, Liu X, Nakayama A, Sugano S, Jiang C-J, Takatsuji H (2012)
  • Author(s): Inoue H, Hayashi N, Matsushita A, Liu X, Nakayama A, Sugano S, Jiang C-J, Takatsuji H
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
• [Presentation] Matsushita A, Inoue H, Goto S, Nakayama A, Sugano S, Hayashi N, Takatsuji H (2012)
  • Author(s): Matsushita A, Inoue H, Goto S, Nakayama A, Sugano S, Hayashi N, Takatsuji H
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
• [Presentation] Blast resistance by panicle blast resistant gene Pb1 is mediated by WRKY45 (2012)
  • Author(s): Inoue H
  • Organizer: XV International Congress on Molecular Plant-Microbe Interactions
  • Place of Presentation: 京都国際会議場
• [Presentation] Pb1による穂いもち抵抗性は転写因子WRKY45の翻訳制御を介している? (2011)
  • Author(s): 井上晴彦
  • Organizer: 日本植物生理学会
  • Place of Presentation: 東北大学 川内北キャンパス
  • Year and Date: 2011-03-20
• [Presentation] Pb1による穂いもち抵抗性は転写因子WRKY45の翻訳後制御を介している? (2011)
  • Author(s): 井上晴彦, 林長生, 松下茜, 中山明, 菅野正治, 姜昌杰, 高辻博志
  • Organizer: 第52回日本植物生理学会年会講演
• [Presentation] 松下茜, 井上晴彦, 後藤新悟, 中山明, 高辻博志 (2011)
  • Author(s): 松下茜, 井上晴彦, 後藤新悟, 中山明, 高辻博志
  • Organizer: 第52回日本植物生理学会年会講演
• [Presentation] 穂いもち圃場抵抗性遺伝子Pb1はゲノムのタンデム重複によって成立した (2011)
  • Author(s): 林長生, 井上晴彦, 高辻博志
  • Organizer: Journal of General Plant Pathology
• [Presentation] Cytokinins act synergistically with salicylic acid to activate defense gene expression in rice (2011)
  • Author(s): Jiang C-J, Shimono M, Sugano S, Kojima M, Liu X, Inoue H, Sakakibara H, Takatsuji H
  • Organizer: 9th International Symposium of Rice Functional Genomics
• [Presentation] Post-transcriptional regulation of WRKY45 by ups-mediated protein degradation (2011)
  • Author(s): Matsushita A, Inoue H, Goto S, Takatsuji H
  • Organizer: 9th International Symposium of Rice Functional Genomics