Analysis of the physiological role of the alternative autophagy in vivo.

• Project/Area Number: 22770054
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: ARAKAWA Satoko 東京医科歯科大学, 難治疾患研究所, 助教 (90415159)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: 微細構造 / オートファジー / 血球分化 / ミトコンドリア / 電子顕微鏡 / オルタナティブ・オートファジー / 赤血球

Research Abstract

We provide evidence that mammalian macroautophagy not only involves an ATG5/ATG7-dependent conventional pathway, but also an ATG5/ATG7-independent alternate pathway. In vivo, Atg5-independent alternative macroautophagy was detected in several embryonic tissues(cf. brain, liver, heart). It also had a function in clearing mitochondria during erythroid maturation.

Research Products

• [Journal Article] Involvement of beclin 1 in engulfment ofapoptotic cells (2012)
  • Author(s): Konishi A, Arakawa S, Yue Z, Shimizu S.
  • Journal Title: J Biol Chem Volume: 287(17) Pages: 13919-13929
• [Journal Article] Shigella targets epithelial tricellular junctions and uses a noncanonical clathrin-dependent endocytic pathway to spread between cells (2012)
  • Author(s): Fukumatsu M, Ogawa M, Arakawa S, Suzuki M, Nakayama K, Shimizu S, KimM, Mimuro H, Sasakawa C.
  • Journal Title: Cell Host Microbe Volume: 11(4) Pages: 325-336
• [Journal Article] Involvement of Beclin 1 in the engulfment of apoptotic cells (2012)
  • Author(s): Konishi A, Arakawa S, Yue Z, Shimizu S
  • Journal Title: J.Biol.Chem. Volume: (epub) Issue: 17 Pages: 13919-13929
  • DOI: 10.1074/jbc.m112.348375
  • Peer Reviewed
• [Journal Article] Shigella targets epithelial tricellular junctions to spread between cells via a noncanonical clathrin-dependent endocytic pathway. (2012)
  • Author(s): Makoto Fukumatsu
  • Journal Title: Cell Host & Microbe Volume: 11 Issue: 4 Pages: 325-336
  • DOI: 10.1016/j.chom.2012.03.001
  • Peer Reviewed
• [Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex (2011)
  • Author(s): Yamasaki T, Kawasaki H, Arakawa S, Shimizu K, Shimizu S, Reiner O, Okano H, Nishina S, Azuma N, Penninger JM, Katada T, Nishina H.
  • Journal Title: J Neurosci Volume: 31(46) Pages: 16872-16883
• [Journal Article] Spatial coupling of mTOR and autophagy augments secretory phenotypes (2011)
  • Author(s): Narita M, Young AR, Arakawa S, Samarajiwa SA, Nakashima T, Yoshida S, Hong S, Berry LS, Reichelt S, Ferreira M, Tavare S, Inoki K, Shimizu S, Narita M.
  • Journal Title: Science Volume: 332(6032) Pages: 966-970
• [Journal Article] Spatial coupling of mTOR and autophagy augments secretory/phenotypes (2011)
  • Author(s): Masako Narita
  • Journal Title: Science Volume: 332 Issue: 6032 Pages: 966-970
  • DOI: 10.1126/science.1205407
  • Peer Reviewed
• [Journal Article] Stress-activated protein kinase MKK7 regulates axon elongation in the developing cerebral cortex (2011)
  • Author(s): Yamasaki T, et al
  • Journal Title: J.Neurosci Volume: 31 Issue: 46 Pages: 16872-16883
  • DOI: 10.1523/jneurosci.1111-11.2011
  • Peer Reviewed
• [Journal Article] Preparation of connexin43-integrated giant Liposomes by a baculovirus expression-liposome fusion method (2010)
  • Author(s): Kamiya K, Tsumoto K, Arakawa S, Shimizu S, Morita I, Yoshimura T, Akiyoshi K
  • Journal Title: Biotechnol Bioeng Volume: 107(5) Pages: 836-843
• [Journal Article] xCT deficiency accelerates chemically induced tumorigenesis (2010)
  • Author(s): Nabeyama A, Kurita A, Asano K, Miyake Y, Yasuda T, Miura I, Nishitai G, ArakawaS, Shimizu S, Wakana S, Yoshida H, Tanaka M.
  • Journal Title: Proc Natl Acad Sci USA Volume: 107(14) Pages: 6436-6441
• [Journal Article] Hypertrophy and unconventional cell division of hepatocytes underlie liver regeneration
  • Author(s): Miyaoka Y, Ebato K, Kato H, Arakawa S, Shimizu S, and Miyajima A.
  • Journal Title: Curr Biol Volume: (In press)
• [Presentation] Analysis of programmed cell death during interdigital web development (2011)
  • Author(s): Arakawa S, Yoshino I, Tsujimoto Y, Shimizu S
  • Organizer: 分子生物学会
  • Place of Presentation: 横浜(パシフィコ横浜)
  • Year and Date: 2011-12-16
• [Presentation] Analysis of programmed cell death duringinterdigital web development
  • Author(s): Arakawa S, Yoshino I, Tsujimoto Y, Shimizu S.
• [Book] Autophagy takes an alternativepathway (2010)
  • Author(s): Shimizu S, Arakawa S, Nishida Y
  • Publisher: Autophagy
• [Book] 新しいオートファジー機構の発見Discovery of Novel Autophagy Pathway (2010)
  • Author(s): 清水重臣荒川聡子西田友哉
  • Publisher: DENTAL DIAMOND
• [Book] Atg5やAtg7を必要としない新規オートファジー機構の発見(カレントトピックス)実験医学 (2010)
  • Author(s): 荒川聡子、西田友哉、清水重臣
• [Book] Atg5やAtg7を必要としないオートファジー機構の発見(HOT PRESS)細胞工学 (2010)
  • Author(s): 西田友哉、荒川聡子、清水重臣
• [Remarks]
  • URL: http://www.tmd.ac.jp/mri/pcb/research-content.html