Analyses of functional RNAs and target genes involves in leg regeneration using transgenic cricket, Gryllus bimaculatus
| Project/Area Number |
22770057
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Morphology/Structure
|
|---|
| Research Institution | The University of Tokushima |
|---|
Principal Investigator |
NAKAMURA Taro 徳島大学, 産学官連携推進部, 講師 (80548834)
|
|---|
| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
|
|---|
| Keywords | 形態形成 / 組織再生 / 機能性RNA / 形質転換技術 / TALEN / ZFN / Flp/FRT / トランスジェニックコオロギ / Zinc Finger Nuclease / Ga14/UAS |
|---|
| Research Abstract |
We cloned several miRNAs from libraries of embryonic and regenerating tissues and observed these expressions in the embryos. We revealed expression patterns of cloned miRNAs. We found miR-8, which is a target of Wnt component gene in our miRNAs libraries. Moreover, to analyze the function of miRNA and its target genes, we established cell trajectory analysis using the transgenic cricket. Furthermore, we established gene-targeting systems using zinc finger nucleases(ZFNs) and transcription activator-like(TAL) effector nucleases(TALENs). Using these systems, we are now trying to investigate the function of the miRNA involves in the leg regeneration in cricket.
|
Report
(3 results)
Research Products
(36 results)
