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Cryptic variation in insect female genitalia: establishment and applications of their observational techniques

Research Project

Project/Area Number 22770058
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Morphology/Structure
Research InstitutionKeio University

Principal Investigator

KAMIMURA Yoshitaka  慶應義塾大学, 商学部, 准教授 (50366952)

Project Period (FY) 2010-04-01 – 2014-03-31
Project Status Completed (Fiscal Year 2013)
Budget Amount *help
¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥260,000 (Direct Cost: ¥200,000、Indirect Cost: ¥60,000)
Fiscal Year 2012: ¥390,000 (Direct Cost: ¥300,000、Indirect Cost: ¥90,000)
Fiscal Year 2011: ¥520,000 (Direct Cost: ¥400,000、Indirect Cost: ¥120,000)
Fiscal Year 2010: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords交尾器 / 顕微鏡観察技術 / 性選択 / 性的対立 / 昆虫 / 進化 / 交尾器形態
Research Abstract

Animal genitalia of internal fertilizers generally show a rapid evolutionary rate compared with other morphological characters. However, compared to interspecific diversities in male genitalia, female genitalia, which usually consist of soft structures without conspicuous landmarks, received less attention because of the difficulty of observation. In this study, efficient methods to detect and describe diversities in female genital traits were explored using in-copula samples of various insect taxa. As the result, a mixture of benzyl alcohol and benzyl benzoate (BABB) has been proven to be highly effective to make insect exoskeleton transparent. The method revealed that female genitalia generally show a sizable structural diversity in their soft parts corresponding to the species-specific male genitalia. Experiments showed that the structural fitting between male and female genitalia reduces mating costs for females such caused by copulatory wounding and other mechanisms.

Report

(5 results)
  • 2013 Annual Research Report   Final Research Report ( PDF )
  • 2012 Annual Research Report
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (30 results)

All 2014 2013 2012 2011 2010 Other

All Journal Article (16 results) (of which Peer Reviewed: 16 results) Presentation (14 results) (of which Invited: 3 results)

  • [Journal Article] Duplicated female receptacle organs for traumatic insemination in the tropical bed bug Cimex hemipterus : Adaptive variation or malformation?2014

    • Author(s)
      Kamimura, Y., Mitsumoto, H., and Lee, C. -Y.
    • Journal Title

      PLoS ONE

      Volume: 9(2) Issue: 2 Pages: e89265-e89265

    • DOI

      10.1371/journal.pone.0089265

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Pre- and postcopulatory sexual selection and the evolution of sexually dimorphic traits in earwigs (Dermaptera)2014

    • Author(s)
      Kamimura, Y
    • Journal Title

      Entomological Science

      Volume: 17 Issue: 2 Pages: 139-166

    • DOI

      10.1111/ens.12058

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Female penis, male vagina, and their correlated evolution in a cave insect2014

    • Author(s)
      Yoshizawa, K., Ferreira, RL, Kamimura, Y., and Lienhard, C
    • Journal Title

      Current Biology

      Volume: 24 Issue: 9 Pages: 1006-1010

    • DOI

      10.1016/j.cub.2014.03.022

    • NAID

      120005468155

    • Related Report
      2013 Annual Research Report 2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mating and genital coupling in the primitive earwig species Echinosoma denticulatum (Pygidicranidae) : imply -cations for genital evolution in dermapteran phylogeny2014

    • Author(s)
      Kamimura, Y. and Lee, C. -Y
    • Journal Title

      Arthlopod System -atics and Phylogeny

      Volume: 72 Pages: 11-21

    • URL

      http://www.senckenberg.de/files/content/forschung/publikationen/arthropodsystematics/asp_72_1/02_asp_72_1_kamimura_11-21.pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric mismatch in strain-specific genital morphology causes increased harm to Drosophila females2014

    • Author(s)
      Masly, J. P., and Kamimura, Y
    • Journal Title

      Evolution

      Volume: (in press) Pages: n/a-n/a

    • DOI

      10.1111/evo.12436

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Mating and genital coupling in the primitive earwig species Echinosoma denticulatum (Pygidicranidae): implications for genital evolution in dermapteran phylogeny.2014

    • Author(s)
      Kamimura, Y., Lee, C.-Y.
    • Journal Title

      Arthropod Systematics & Phylogeny

      Volume: 72 Pages: 11-21

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Asymmetric mismatch in strain-specific genital morphology causes increased harm to Drosophila females.2014

    • Author(s)
      Masly, J. P., Kamimura, Y.
    • Journal Title

      Evolution

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Lock-and-key structural isolation between sibling Drosophila species2012

    • Author(s)
      Kamimura, Y., and Mitsumoto, H
    • Journal Title

      Entomological Science

      Volume: 15 Issue: 2 Pages: 197-201

    • DOI

      10.1111/j.1479-8298.2011.00490.x

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Correlated evolutionary changes in Drosophila female genitalia reduce the possible infection risk caused by male copulatory wounding2012

    • Author(s)
      Kamimura, Y
    • Journal Title

      Behavioral Ecology and Sociobiology

      Volume: 66 Issue: 8 Pages: 1107-1117

    • DOI

      10.1007/s00265-012-1361-0

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genital coupling and copulatory wounding in Drosophila teissieri (Diptera : Drosophilidae)2012

    • Author(s)
      Kamimura, Y., and Mitsumoto, H
    • Journal Title

      Canadian Journal of Zoology

      Volume: 90 Issue: 12 Pages: 1437-1440

    • DOI

      10.1139/cjz-2012-0186

    • Related Report
      2013 Final Research Report 2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] キイロショウジョウバエ種群における交尾器形態の進化 : その機能研究に関するレビュー2011

    • Author(s)
      上村 佳孝・三本 博之
    • Journal Title

      低温科学

      Volume: 69 Pages: 39-50

    • URL

      http://www.lowtem.hokudai.ac.jp/lts/LTS69.pdf

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Does surgical manipulation of Drosophila intromittent organs affect insemination success?2011

    • Author(s)
      Kamimura, Y., and Polak, M
    • Journal Title

      Proceedings of the Royal Society of London B

      Volume: 278 Issue: 1707 Pages: 815-816

    • DOI

      10.1098/rspb.2010.2431

    • Related Report
      2013 Final Research Report
    • Peer Reviewed
  • [Journal Article] Population stochasticity, random determination of handedness, and the genetic basis of antisymmetry2011

    • Author(s)
      Kamimura, Y
    • Journal Title

      Journal of Theoretical Biology

      Volume: 290 Pages: 73-80

    • DOI

      10.1016/j.jtbi.2011.08.021

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Comparative copulation anatomy of the Drosophila melanogaster species complex (Diptera : Drosophilidae)2011

    • Author(s)
      Kamimura, Y., and Mitsumoto, H
    • Journal Title

      Entomological Science

      Volume: 14 Issue: 4 Pages: 399-410

    • DOI

      10.1111/j.1479-8298.2011.00467.x

    • NAID

      10030580869

    • Related Report
      2013 Final Research Report 2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Does surgical manipulation of Drosophila intromittent organs affect insemination success?2011

    • Author(s)
      Kamimura, Y., Polak, M
    • Journal Title

      Proceedings of the Royal Society of London B

      Volume: Vol.278 Pages: 815-816

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] キイロショウジョウバエ種群における交尾器形態の進化:その機能研究に関するレビュー2011

    • Author(s)
      上村佳孝、三本博之
    • Journal Title

      低温科学

      Volume: 69巻 Pages: 39-50

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] Asymmetric mismatch in secondary genital morphology increases harm to Drosophila females2014

    • Author(s)
      Masly, J. P., Kamimura, Y.
    • Organizer
      Evolution 2014
    • Place of Presentation
      Raleigh Convention Center, North Carolina, USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] 交尾器進化を巡る論争 : Drosophila は解決できるか?2013

    • Author(s)
      上村 佳孝
    • Organizer
      第1回ショウジョウバエ多様性研究会シンポジウム
    • Place of Presentation
      三島市 : 国立遺伝学研究所
    • Year and Date
      2013-09-30
    • Related Report
      2013 Final Research Report
  • [Presentation] アナナスショウジョウバエ類野外集団における種間浸透-ペナン島(マレーシア)の事例2013

    • Author(s)
      澤村 京一・佐藤 玄・李 昭揚・上村佳孝・松田 宗男
    • Organizer
      第1回ショウジョウバエ多様性研究会シンポジウム
    • Place of Presentation
      三島市 : 国立遺伝学研究所
    • Year and Date
      2013-09-30
    • Related Report
      2013 Final Research Report
  • [Presentation] アナナスショウジョウバエ類の野外集団における一方向的な種間浸透:ペナン島(マレーシア)の事例2013

    • Author(s)
      澤村 京一・佐藤 玄・李 昭揚・上村 佳孝・松田 宗男
    • Organizer
      日本進化学会第15回大会
    • Place of Presentation
      茨城県つくば市(筑波大学)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 交尾器形態の不一致がもたらすメスの交尾コスト-種間交尾を用いた検証-2012

    • Author(s)
      上村 佳孝
    • Organizer
      日本昆虫学会第72回大会
    • Place of Presentation
      東京都町田市 : 玉川大学
    • Year and Date
      2012-09-17
    • Related Report
      2013 Final Research Report
  • [Presentation] Kamimura Y. and Mitsumoto H. Comparative copulation anatomy of the Drosophila melanogaster species complex (Diptera : Drosophilidae)2012

    • Author(s)
      上村 佳孝, 三本 博之
    • Organizer
      日本昆虫学会第72回大会
    • Place of Presentation
      東京都町田市 : 玉川大学
    • Year and Date
      2012-09-16
    • Related Report
      2013 Final Research Report
  • [Presentation] 学会賞受賞講演:Kamimura Y. and Mitsumoto H. Comparative copulation anatomy of the Drosophila melanogaster species complex (Diptera: Drosophilidae).2012

    • Author(s)
      上村佳孝
    • Organizer
      日本昆虫学会第72回大会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 交尾器形態の不一致がもたらすメスの交尾コスト―種間交尾を用いた検証―2012

    • Author(s)
      上村佳孝
    • Organizer
      日本昆虫学会第72回大会
    • Place of Presentation
      玉川大学(東京都町田市)
    • Related Report
      2012 Annual Research Report
  • [Presentation] ショウジョウバエ2種間の交尾器形態の違いによる生殖的隔離2011

    • Author(s)
      上村 佳孝, 三本 博之
    • Organizer
      日本昆虫学会第69回大会
    • Place of Presentation
      長野県松本市 : 信州大学
    • Year and Date
      2011-09-18
    • Related Report
      2013 Final Research Report
  • [Presentation] ショウジョウバエ2種間の交尾器形態の違いによる生殖的隔離2011

    • Author(s)
      上村佳孝、三本博之
    • Organizer
      日本昆虫学会第69回大会
    • Place of Presentation
      長野県松本市(信州大学)
    • Year and Date
      2011-09-18
    • Related Report
      2011 Annual Research Report
  • [Presentation] The Drosophila melanogaster species subgroup における交尾器進化2010

    • Author(s)
      上村 佳孝
    • Organizer
      北海道大学低温科学研究所ワークショップ「ショウジョウバエ研究のいまとこれから」
    • Place of Presentation
      札幌市 : 北海道大学
    • Year and Date
      2010-09-23
    • Related Report
      2013 Final Research Report
  • [Presentation] The Drosophila melanogaster species subgroupにおける交尾器進化2010

    • Author(s)
      上村佳孝
    • Organizer
      北海道大学低温科学研究所ワークショップ「ショウジョウバエ研究のいまとこれから」
    • Place of Presentation
      札幌(北海道大学・低温科学研究所)
    • Year and Date
      2010-09-23
    • Related Report
      2010 Annual Research Report
  • [Presentation] 交尾器進化を巡る論争:Drosophilaは解決できるか?

    • Author(s)
      上村 佳孝
    • Organizer
      第1回ショウジョウバエ多様性研究会シンポジウム
    • Place of Presentation
      静岡県三島市(国立遺伝学研究所)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] アナナスショウジョウバエ類野外集団における種間浸透―ペナン島(マレーシア)の事例

    • Author(s)
      澤村 京一・佐藤 玄・李 昭揚・上村 佳孝・松田 宗男
    • Organizer
      第1回ショウジョウバエ多様性研究会シンポジウム
    • Place of Presentation
      静岡県三島市(国立遺伝学研究所)
    • Related Report
      2013 Annual Research Report
    • Invited

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Published: 2010-08-23   Modified: 2019-07-29  

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