| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Research Abstract |
In this study, 41 proteins were identified as chloroplast nucleoid-associated proteins by LC-MS/MS analyses. And then, I analyzed about two high content unknown function proteins, cpNUC1 and cpNUC3. I found that (1) cpNUC1 protein has an ability of DNA compaction in vitro but is uninvolved in the cpDNA segregation, and that (2) cpNUC3 is involved in hidden break of rrn23 RNA but is uninvolved in the cpDNA segregation. In addition, I revealed that chloroplast DNA replication is regulated by the redox rather than chloroplast division/cell cycle
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