Localization of vacuolar H+-pyrophosphatase and vacuole biogenesis in plant cells

• Project/Area Number: 22770063
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Morphology/Structure
• Research Institution: The Institute of Physical and Chemical Research
• Principal Investigator: NISHIKAWA Mayuko 独立行政法人理化学研究所, 機能開発研究グループ, 技師 (80550376)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 植物 / 電子顕微鏡 / 液胞 / 液胞膜 / 高圧凍結技法 / タバコ / シロイヌナズナ / H+-ピロホスファターゼ / H^+-ピロホスファターゼ

Research Abstract

To investigate how plant vacuoles are generated, I examined Arabidopsis and Tobacco root tip tissues by electron microscopy. The anti-vacuolar H^+-pyrophosphatase (V-PPase) antibody was used for immunogold labeling, and positive signals in the vacuolar membrane and a ring-like structure were detected especially in the procambium. Some ring-like structures fused with vacuoles that containing homogenous particle. Vacuole genesis of the procambium may different from other cell layer of root.

Research Products

• [Journal Article] Regulation of root greening by light and auxin/cytokinin signaling in Arab idopsis. (2012)
  • Author(s): Kobayashi, K., Baba, S., Obayashi, T. Sato, M., Toyooka, K., Keranen, M., Aro, E. -M, Fukaki, H., Ohta, E., Sugimoto K., Masuda T.
  • Journal Title: Plant Cell Volume: 24 Issue: 3 Pages: 1081-1095
  • DOI: 10.1105/tpc.111.092254
  • Peer Reviewed
• [Journal Article] The Ets Transcription Factor Spi-B Is Essential for the Differentiation of Intestinal Microfold (M) Cells. (2012)
  • Author(s): Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., Hemmi, H., Knoop, K.A., Kumar, N., Sato, M., Katsuno, T., Yokosuka, O,, Toyooka, K,, Nakai, K., Sakamoto, A., Kitahara, Y., Jinnohara, T., McSorley, S.J., Kaisho, T., Williams, I.R., Ohno, H.
  • Journal Title: Nature Immunology Volume: 13 Pages: 729-736
  • Peer Reviewed
• [Journal Article] Micro-CT observations of the 3D distribution of calcium oxalate crystals in cotyledons during maturation and germination in Lotus miyakojimae seeds. (2012)
  • Author(s): Yamauchi, D., Tamaoki, D., Hayami, M., Takeuchi, M., Karahara, I., Sato, M., Toyooka, K., Nishioka, H., Terada, Y., Uesugi, K., Takano, H., Kagoshima, Y., Mineyuki, Y.
  • Journal Title: Journal of Electron Microscopy Volume: (印刷中) Issue: 3 Pages: 353-361
  • DOI: 10.1093/jmicro/dfs079
  • Peer Reviewed
• [Journal Article] Ultrastructure of the rickettsial endosymbiont "MIDORIKO"in the green alga Carteria cerasiformis as revealed by high-pressure freezing and freeze-substitution fixation. (2012)
  • Author(s): Kawafune, K. , Sato, M., Toyooka, K. Nozaki, H
  • Journal Title: Protoplasma Volume: (印刷中) Issue: 4 Pages: 949-953
  • DOI: 10.1007/s00709-012-0469-4
  • Peer Reviewed
• [Journal Article] The Ets transcription factor Spi-B is essential for the differentiation of intestinal microfold cells (2012)
  • Author(s): Kanaya, T., Hase, K., Takahashi, D., Fukuda, S., Hoshino, K., Sasaki, I., Hemmi, H., Knoop, K. A., Kumar, N., Sato, M., Katsuno, T., Yokosuka, O., Toyooka, K., Nakai, K., Sakamoto, A., Kitahara, Y., Jinnohara, T., McSorley, S. J., Kaisho, T., Williams, I. R., and Ohno, H.
  • Journal Title: Nat Immunol Volume: 13 Issue: 8 Pages: 729-736
  • DOI: 10.1038/ni.2352
  • Peer Reviewed
• [Journal Article] Plasma membrane aquaporin AqpZ protein is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp (2011)
  • Author(s): Akai, M., Onai, K., Kusano, M., Sato, M., Redestig, H., Toyooka, K., Morishita, M., Miyake, H., Hazama, A., Checchetto, V., Szabo, I., Matsuoka, K., Saito, K., Yasui, M., Ishiura, M., and Uozumi, N
  • Journal Title: PCC 6803. J. Biol Volume: 286 Issue: 28 Pages: 25224-25235
  • DOI: 10.1074/jbc.m111.236380
  • Peer Reviewed
• [Journal Article] AUNGUSTIFOLIA, a plant homolog of CtBP/BARS, functions outside the nucleus (2011)
  • Author(s): Minamisawa N, Sato M, Cho K-H, Ueno H, Takeuchi K, Kajiwara M, Yamato KT, Ohyama K, Toyooka K, Kim G-T, Horiguchi G, Takano H, Ueda T, and Tsukaya H
  • Journal Title: Plant J Volume: 68 Issue: 5 Pages: 788-799
  • DOI: 10.1111/j.1365-313x.2011.04731.x
  • Peer Reviewed
• [Journal Article] Immunohistochemical observation of indole-3-acetic acid at the IAA synthetic maize coleoptile tips. (2011)
  • Author(s): Nishimura, T., Toyooka, K., Sato, M., Matsumoto, S., Lucas, M.M., Strnad, M., Baluska, F., Koshiba, T.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 2013-2022
  • URL: http://www.ncbi.nlm.nih.gov/pubmed/22112455
  • Peer Reviewed
• [Journal Article] Immunohistochemical observation of indole-3-acetic acid at the IAA synthetic maize coleoptile tips (2011)
  • Author(s): Nishimura, T.
  • Journal Title: Plant Signaling & Behavior Volume: 6 Pages: 2013-2022
  • Peer Reviewed
• [Journal Article] Plasma membrane aquaporin AqpZ is essential for glucose metabolism during photomixotrophic growth of Synechocystis sp.PCC 6803 (2011)
  • Author(s): Akai, M.
  • Journal Title: Journal Biological Chemistry Volume: 286 Pages: 25224-25235
  • Peer Reviewed
• [Journal Article] Isolation of Chloroplast FtsZ and AtpC, and Analyses of Protein Targeting into the Complex Chloropast of Haptophyta Pavlova pinguis. (2010)
  • Author(s): Nishikawa, T., Kajitani, H., Sato, M., Mogi, Y., Moriyama, Y. and Kawano, S.
  • Journal Title: Cytologia Volume: 75 Pages: 203-210
  • URL: https://www.jstage.jst.go.jp/article/cytologia/75/2/75_2_203/_article
  • Peer Reviewed
• [Journal Article] Isolation of Chloroplast FtsZ and AtpC, and Analyses of Protein Targeting into the Complex Chloroplast of Haptophyta Pavlova pinguis (2010)
  • Author(s): Nishikawa, T.
  • Journal Title: Cytologia Volume: 75 Pages: 203-210
  • Peer Reviewed
• [Presentation] 電顕アトラス:高圧凍結技法を取り入れた広域電顕像撮影システムの開発 (2013)
  • Author(s): 佐藤繭子、朽名夏麿、澤木史江、若崎眞由美、桧垣匠、吉田拓弘、桜井哲也、馳澤盛一郎、持田恵一、永田典子、豊岡公徳
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山市
  • Year and Date: 2013-03-21
• [Presentation] V-PPase の細胞内局在から見るタバコ根端分裂組織の液胞形成 (2012)
  • Author(s): 佐藤繭子、後藤友美、松岡健、豊岡公徳
  • Organizer: 第68回日本顕微鏡学会学術講演会
  • Place of Presentation: つくば市
  • Year and Date: 2012-05-15
• [Presentation] タバコ根端組織における液胞型プロトンピロホスファターゼの細胞内局在 (2012)
  • Author(s): 佐藤繭子、後藤友美、松岡健、豊岡公徳
  • Organizer: 第53回日本植物生理学会年
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-18
• [Presentation] タバコ根端組織における液胞型プロトンピロホスファターゼの細胞内局在 (2012)
  • Author(s): 佐藤繭子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都市
  • Year and Date: 2012-03-18
• [Presentation] タバコ根端組織における液胞形成:液胞型プロトンピロホスファターゼの細胞内局在 (2011)
  • Author(s): 佐藤繭子、後藤友美、豊岡公徳
  • Organizer: 第23回日本植物形態学会大会
  • Place of Presentation: 東京都文京区
  • Year and Date: 2011-09-16
• [Presentation] 高圧凍結技法を用いた植物液胞形成過程の微細構造解析 (2011)
  • Author(s): 佐藤繭子、後藤友美、豊岡公徳
  • Organizer: 第67回日本顕微鏡学会学術講演会・シンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 2011-05-17
• [Presentation] 高圧凍結技法を用いた植物液胞形成過程の微細構造解析 (2011)
  • Author(s): 佐藤繭子
  • Organizer: 第67回日本顕微鏡学会年会・シンポジウム
  • Place of Presentation: 福岡市
  • Year and Date: 2011-05-17
• [Presentation] 植物根端の液胞形成における新奇構造体の解析 (2011)
  • Author(s): 佐藤繭子、後藤友美、豊岡公徳、松岡健
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台
  • Year and Date: 2011-03-22
• [Presentation] 植物根端の液胞形成における新奇構造体の解析 (2011)
  • Author(s): 佐藤繭子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 仙台市
  • Year and Date: 2011-03-22
• [Presentation] Immunocytochemical Analysis of vacuole formation process by using high-pressure freezing technique (2010)
  • Author(s): Mayuko Sato, Yumi Goto, Ken Matsuoka, Kiminori Toyooka.
  • Organizer: International Microscopy Congress 17
  • Place of Presentation: Brazil, Rio de Janeiro
  • Year and Date: 2010-09-23
• [Presentation] Immunocytochemical Analysis Of Vacuole Formation Process By Using High-Pressure Freezing Technique (2010)
  • Author(s): Mayuko Sato
  • Organizer: International Microscopy Congress 17
  • Place of Presentation: Brazil, Rio de Janeiro
  • Year and Date: 2010-09-23
• [Presentation] 植物根端の液胞形成における新奇構造体の解析 (2010)
  • Author(s): 佐藤繭子、河合たか子、若崎眞由美、松岡健、豊岡公徳
  • Organizer: 第66回日本顕微鏡学会学術講演
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-05-24
• [Presentation] 植物根端の液胞形成における新奇構造体の解析 (2010)
  • Author(s): 佐藤繭子
  • Organizer: 第66回日本顕微鏡学会学術講演会
  • Place of Presentation: 名古屋市
  • Year and Date: 2010-05-24
• [Presentation] 電顕アトラス：高圧凍結技法を取り入れた広域電顕像撮影システムの開発
  • Author(s): 豊岡公徳
  • Organizer: 第54回日本植物生理学会年会
  • Place of Presentation: 岡山市 岡山大学津島キャンパス
• [Presentation] V-PPaseの細胞内局在から見るタバコ根端分裂組織の液胞形成
  • Author(s): 佐藤繭子
  • Organizer: 第68回日本顕微鏡学会年会
  • Place of Presentation: つくば市 つくば国際会議場
• [Presentation] タバコ根端分裂組織における液胞膜タンパク質V-PPaseの分布と液胞形成
  • Author(s): 佐藤繭子
  • Organizer: 第69回日本顕微鏡学会年会
  • Place of Presentation: 吹田市 ホテル阪急エキスポパーク