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The mechanism for length regulation of thick filament by muscle elastic protein connectin

Research Project

Project/Area Number 22770065
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Animal physiology/Animal behavior
Research InstitutionChiba University

Principal Investigator

HANASHIMA Akira  千葉大学, 大学院・理学研究科, 特任研究員 (70572981)

Project Period (FY) 2010 – 2012
Project Status Completed (Fiscal Year 2012)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Keywordsコネクチン / 横紋筋 / 弾性タンパク質 / 巨大タンパク質 / サルコメア / タイチン / ミオシン / 分子定規 / 太いフィラメント
Research Abstract

We found a novel protein that binds to muscle elastic protein connectin. Our analysis indicates this protein locates at the edge of myosin filament and neuron-muscular junction on striated muscles, and the gene of this protein includes twenty-five exons and produces five splicing isoforms. Additionally, we identified the binding proteins and a break-down protein of novel protein, and also found the existence of this protein in various animals including human and Drosophila.

Report

(4 results)
  • 2012 Annual Research Report   Final Research Report ( PDF )
  • 2011 Annual Research Report
  • 2010 Annual Research Report
  • Research Products

    (34 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (25 results) (of which Invited: 1 results)

  • [Journal Article] Regulation of nicotinic acetylcholine receptor turnover by MuRF1 connects muscle activity to endo/lysosomal and atrophy pathways2013

    • Author(s)
      Rudolf R, Bogomolovas J, Strack S, Choi K, Khan MM, Wagner A, Brohm K, Hanashima A, Gasch A, Labeit D, Labeit S
    • Journal Title

      AGE

      Volume: (in press) Issue: 5 Pages: 1663-1674

    • DOI

      10.1007/s11357-012-9468-9

    • Related Report
      2012 Annual Research Report 2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Actin capping proteins, CapZ (beta-actinin) and tropomodulin in amphioxus striated muscle2012

    • Author(s)
      Bao Y, Kake T, Hanashima A, Nomiya Y, Kubokawa K, Kimura S
    • Journal Title

      GENE

      Volume: 510 Pages: 78-86

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Genomic- and protein-based approaches for connectin (titin) identification in the ascidian Ciona intestinalis2012

    • Author(s)
      Hanashima, A., Ogasawara, M., Nomiya, Y., Sasaki, T., Bao, Y., Kimura, S.
    • Journal Title

      Methods

      Volume: Vol. 56(1) Issue: 1 Pages: 18-24

    • DOI

      10.1016/j.ymeth.2011.12.010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Actin capping proteins, CapZ (β-actinin) and tropomodulin in amphioxus striated muscle2012

    • Author(s)
      Bao, Y., Kake, T., Hanashima, A., Nomiya, U., Kubokawa, K. and Kimura, S.
    • Journal Title

      Gene

      Volume: Vol. 510 (1) Issue: 1 Pages: 78-86

    • DOI

      10.1016/j.gene.2012.07.081

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomic- and protein-based approaches for connectin (titin)identification in the ascidian Ciona intestinalis2012

    • Author(s)
      Hanashima, et al.
    • Journal Title

      Methods

      Volume: 56 Pages: 18-24

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Amphioxus connectin exhibits merged structure as invertebrate connectin in I-band region and vertebrate connectin in A-band region2011

    • Author(s)
      Ohtsuka, S., Hanashima, A., Kubokawa, K., Bao, Y., Tando, Y., Kohmaru, J., Nakaya, H., Maruyama, K. and Kimura, S.
    • Journal Title

      J. Mol. Biol

      Volume: Vol. 409 (3) Issue: 3 Pages: 415-426

    • DOI

      10.1016/j.jmb.2011.04.010

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Amphioxus connectin exhibits merged structure as invertebrate connectin in I-band region and vertebrate connectin in A-band region2011

    • Author(s)
      Ohtsuka, et al.
    • Journal Title

      J Mol Biol

      Volume: 409 Pages: 415-426

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin2010

    • Author(s)
      Chitose, R., Watanabe, A., Asano, M., Hanashima, A., Sasano, K., Bao, Y.,Maruyama, K. and Kimura, S.
    • Journal Title

      J. Biomed. Biotechnol

      Volume: 2010 Pages: 108495-108495

    • DOI

      10.1155/2010/108495

    • Related Report
      2012 Final Research Report
    • Peer Reviewed
  • [Journal Article] Isolation of Nebulin from Rabbit Skeletal Muscle and Its Interaction with Actin2010

    • Author(s)
      Chitose, et al.
    • Journal Title

      J Biomed Biotechnol

      Volume: 2010 Pages: 108495-108495

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Presentation] プラナリアのコネクチン様タンパク質の配列2012

    • Author(s)
      佐々野耕平、○倉沢正義、花島章、包玉龍、中内祐二、木村澄子
    • Organizer
      日本生物物理学会第50回年会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 軟体動物横紋筋のコネクチン様タンパク質2012

    • Author(s)
      ○包玉龍、花島章、袖山文彰、木村澄子
    • Organizer
      日本生物物理学会第50回年会
    • Place of Presentation
      名古屋
    • Year and Date
      2012-09-22
    • Related Report
      2012 Final Research Report
  • [Presentation] 脊椎動物のゲノム中におけるコネクチン遺伝子数の変遷2012

    • Author(s)
      ○花島章、木村澄子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2012 Final Research Report
  • [Presentation] ホヤ心筋ネブリンの一次構造解析2012

    • Author(s)
      ○西川彰、浜岡知哉、小笠原道生、花島章、木村澄子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2012 Final Research Report
  • [Presentation] 刺胞動物のコネクチン様タンパク質2012

    • Author(s)
      ○春山綾菜、花島章、木村澄子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2012 Final Research Report
  • [Presentation] ウニのコネクチン様タンパク質2012

    • Author(s)
      ○佐々木智子、松浦哲、花島章、野宮唯、木村澄子
    • Organizer
      日本動物学会第83回大会
    • Place of Presentation
      大阪
    • Year and Date
      2012-09-13
    • Related Report
      2012 Final Research Report
  • [Presentation] Genomic- and Protein-Based Approaches for Connectin-Like Protein Identification in Amphioxus and Ascidian2012

    • Author(s)
      ○ Hanashima A, Bao Y, Kimura S
    • Organizer
      BIT's 3rd World DNA and Genome day
    • Place of Presentation
      Xi'an, China
    • Year and Date
      2012-04-28
    • Related Report
      2012 Final Research Report
  • [Presentation] 無脊椎動物平滑筋のコネクチン様タンパク質2012

    • Author(s)
      ○包玉龍、花島章、松浦哲、木村澄子
    • Organizer
      第49回生体運動研究合同班会議
    • Place of Presentation
      筑波
    • Year and Date
      2012-01-06
    • Related Report
      2012 Final Research Report
  • [Presentation] カイメン、ヒドラのコネクチン様タンパク質の探索2011

    • Author(s)
      日高暢也、○木村澄子、花島章
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] ホヤのコネクチン様タンパク質2011

    • Author(s)
      ○佐々木智子、野宮唯、花島章、包玉龍、小笠原道生、木村澄子
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 扁形動物ナミウズムシDugesiajaponicaのコネクチン様タンパク質2011

    • Author(s)
      ○佐々野耕平、花島章、包玉龍、中内祐二、木村澄子
    • Organizer
      日本動物学会第82回大会
    • Place of Presentation
      旭川
    • Year and Date
      2011-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] コネクチン様タンパク質の多様性2011

    • Author(s)
      ○佐々野耕平、花島章、大塚聡、松浦哲、幸丸純貴、木村澄子
    • Organizer
      第48回生体運動研究合同班会議
    • Place of Presentation
      大阪
    • Year and Date
      2011-01-07
    • Related Report
      2012 Final Research Report
  • [Presentation] Amphioxus striated muscle has beta-actinin and tropomodulin2010

    • Author(s)
      ○ Bao Y, Kake T, Hanashima A,Kubokawa K, Tando Y, Kimura S
    • Organizer
      International Conference on Bioinformatics
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2010-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] The connectin-like protein gene of amphioxus striated muscle2010

    • Author(s)
      Ohtsuka S, Hanashima A, KubokawaK, Tando Y, ○ Kimura S.
    • Organizer
      International Conference on Bioinformatics
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2010-09-26
    • Related Report
      2012 Final Research Report
  • [Presentation] ホタテ貝横紋筋のコネクチン様タンパク質2010

    • Author(s)
      ○包玉龍、黒澤元、花島章、木村澄子
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-25
    • Related Report
      2012 Final Research Report
  • [Presentation] ウニコネクチン様タンパク質のドメイン構造2010

    • Author(s)
      ○松浦哲、花島章、包玉龍、木村澄子
    • Organizer
      日本動物学会第81回大会
    • Place of Presentation
      東京
    • Year and Date
      2010-09-25
    • Related Report
      2012 Final Research Report
  • [Presentation] プラナリアのコネクチン様タンパク質の探索2010

    • Author(s)
      ○佐々野耕平、花島章、中内祐二、木村澄子
    • Organizer
      日本生物物理学会第48回年会
    • Place of Presentation
      仙台
    • Year and Date
      2010-09-21
    • Related Report
      2012 Final Research Report
  • [Presentation] 頭索動物ナメクジウオのトロポモジュリンの同定2010

    • Author(s)
      ○包玉龍、掛武威、花島章、木村澄子
    • Organizer
      第62回日本細胞生物学会大会
    • Place of Presentation
      大阪
    • Year and Date
      2010-05-21
    • Related Report
      2012 Final Research Report
  • [Presentation] Genomic- and Protein-Based Approaches for Connectin-Like Protein Identification in Amphioxus and Ascidian.

    • Author(s)
      Hanashima A
    • Organizer
      BIT’s 3rd World DNA and Genome day.
    • Place of Presentation
      Xi’an, China
    • Related Report
      2012 Annual Research Report
    • Invited
  • [Presentation] 脊椎動物のゲノム中におけるコネクチン遺伝子数の変遷

    • Author(s)
      花島章、木村澄子
    • Organizer
      日本動物学会 第83回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] ウニのコネクチン様タンパク質

    • Author(s)
      佐々木智子、松浦哲、花島章、野宮唯、木村澄子
    • Organizer
      日本動物学会 第83回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 刺胞動物のコネクチン様タンパク質

    • Author(s)
      春山綾菜、花島章、木村澄子
    • Organizer
      日本動物学会 第83回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] ホヤ心筋ネブリンの一次構造解析

    • Author(s)
      西川彰、浜岡知哉、小笠原道生、花島章、木村澄子
    • Organizer
      日本動物学会 第83回大会
    • Place of Presentation
      大阪
    • Related Report
      2012 Annual Research Report
  • [Presentation] 軟体動物横紋筋のコネクチン様タンパク質

    • Author(s)
      包玉龍、花島章、袖山文彰、木村澄子
    • Organizer
      日本生物物理学会 第50回年会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report
  • [Presentation] プラナリアのコネクチン様タンパク質の配列

    • Author(s)
      佐々野耕平、倉沢正義、花島章、包玉龍、中内祐二、木村 澄子
    • Organizer
      日本生物物理学会 第50回年会
    • Place of Presentation
      名古屋
    • Related Report
      2012 Annual Research Report

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Published: 2010-08-23   Modified: 2019-07-29  

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