Is there aneuploid evolution?

• Project/Area Number: 22770078
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Chiba University
• Principal Investigator: KIKUCHI Shinji 千葉大学, 大学院・園芸学研究科, 助教 (80457168)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
• Keywords: トレニア / 染色体 / 種分化 / SSR マーカー / 連鎖地図 / FISH / 遺伝資源 / SSRマーカー

Research Abstract

To identify an extra chromosome observed in interspecific hybrid between Torenia fournieri (2n=2x=18) and Torenia baillonii (2n=2x=16) at DNA level, 169 SSR markers were developed using fosmid and cDNA clone sequences and a partial linkage map was constructed in F_2 population of T. fournieri and T. violacea. Next, fosmid-FISH analysis has been conducted to screen fosmid clones derived from the extra chromosome, but the FISH signals of 19 clones tested did not formed on the extra chromosome. In addition, phylogenic analysis including other 7 Torenia species collected in south Asia suggests a hypothesis that T. baillonii lost the extra chromosome during the speciation.

Research Products

• [Journal Article] Iniguez, Jiming Jiang, Euchromatic subdomains in rice centromeres are associated with genes and transcription. Plant Cell (2011)
  • Author(s): Yufeng Wu, Shinji Kikuchi, HuihuangYan, Wenli Zhang, Heidi Rosenbaum, Leonardo A
  • Journal Title: equal contribution Volume: 23 Pages: 4054-4064
  • Peer Reviewed
• [Journal Article] Effects of gamma-ray irradiation in plant morphology of interspecific hybrids between Torenia fournieri and Torenia baiilonii (2011)
  • Author(s): Wipaporn Sawangmee, Thunya Taychasinpitak, Peeranuch Jompuk, Shinji Kikuchi
  • Journal Title: Kasetsart Journal Volume: 45 Pages: 1-8
  • Peer Reviewed
• [Journal Article] Euchromatic subdomains in rice centromeres are associated with genes and transcription (2011)
  • Author(s): Yufeng Wu
  • Journal Title: Plant Cell Volume: 23 Pages: 4054-4064
  • Peer Reviewed
• [Journal Article] Genome-widemapping of cytosine methylation revealed dynamic DNA methylation patterns associated with genes and centromeres in rice. Plant Journal (2010)
  • Author(s): Huihuang Yan, Shinji Kikuchi, Pavel Neumann, Wenli Zhang, Yufeng Wu, Feng Chen, Jiming Jiang
  • Journal Title: equal contribution Volume: 63 Pages: 353-365
  • Peer Reviewed
• [Journal Article] anovel subtelomeric repeat of Jatropha curcas L. and its use in karyotyping (2010)
  • Author(s): Shinji Kikuchi, Hisashi Tsujimoto, Hidenori Sassa, Takato Koba, JcSat1
  • Journal Title: Chromosome Science Volume: 13 Pages: 11-16
  • Peer Reviewed
• [Journal Article] Apple S locus region represents a large cluster of related, polymorphic and pollen-specific F-box genes (2010)
  • Author(s): Mai Minamikawa, Hiroyuki Kakui, Sanhong Wang, Nobuhiro Kotoda, ShinjiKikuchi, Takato Koba, Hidenori Sassa
  • Journal Title: Plant Molecular Biology Volume: 74 Pages: 143-154
  • Peer Reviewed
• [Journal Article] A simple method of chromosome doubling using colchicines in Torenia (Linderniaceae), and the behavior of meiotic chromosomes in amphidiploids. Chromosome Science
  • Author(s): Jiraporn Jiranapapan, Shinji Kikuchi, Benya Manochai, Thunya Taychasinpitak, Hiroyuki Tanaka, Hisashi Tsujimoto
  • Journal Title: equal contribution Volume: (受理済・印刷中)
  • Peer Reviewed
• [Presentation] An extra chromosome of Torenia fournieri (2012)
  • Author(s): S. Kikuchi
  • Organizer: MUSC joint seminar and graduate forum in science & technology
  • Place of Presentation: タイ・マヒドン大学
  • Year and Date: 2012-03-15
• [Presentation] An extra chromosome of Torenia fournieri (2012)
  • Author(s): Shinji Kikuchi
  • Organizer: 2012 Joint Seminar (CU-KSU-MUSC) in Science and Technology
  • Place of Presentation: Mahidol University (Thailand)(招待講演)
  • Year and Date: 2012-03-15
• [Presentation] Koba 、Meiotic behavior and chromosome number variation in amphidiploid between Triticum durum and Aegilops uniaristata (2012)
  • Author(s): K. Nagataki, S. Kikuchi, K. Mishina,
  • Organizer: MUSC joint seminar and graduate forum in science & technology
  • Place of Presentation: タイ・マヒドン大学
  • Year and Date: 2012-03-06
• [Presentation] イネ動原体におけるユークロマティックなヒストン修飾 (2011)
  • Author(s): Wu Y, Kikuchi S, Yan H, Zhang W, Rosenbaum H, Iniguez L, Jiang J
  • Organizer: 染色体学会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-11-13
• [Presentation] イネ動原体におけるユークロマティックなヒストン修飾 (2011)
  • Author(s): Wu Y., Kikuchi S., Yan H., Zhang W.,Rosenbaum H., Iniguez L., Jiang J
  • Organizer: 第62回染色体学会年会
  • Place of Presentation: 神奈川大学
  • Year and Date: 2011-11-11
• [Presentation] Takato Koba 、Structural features of the S locus od Apple 、International workshop (2011)
  • Author(s): Hidenori Sassa, Sanhong Wang, MaiMinamikawa, Hiroyuki Kakui, ShinjiKikuchi
  • Organizer: Floral biology and S-incompatibility in fruit species
  • Place of Presentation: イタリア・ボローニャ大学
• [Presentation] イネゲノムにおけるDNAメチル化解析 (2010)
  • Author(s): Huihuang Yan, Shinji Kikuchi, Pavel Neumann, Zhang Wenli, Wu Yufeng, Chen Feng, Jiang Jiming
  • Organizer: 第61回染色体学会年会
  • Place of Presentation: 東邦大学
  • Year and Date: 2010-11-07
• [Presentation] 花卉園芸植物トレニア属における分子系統解析と染色体数 (2010)
  • Author(s): 菅野健太
  • Organizer: 染色体学会
  • Place of Presentation: 東邦大学(千葉県)
  • Year and Date: 2010-11-05
• [Presentation] Hidenori Sassa、S-RNase and SFBB family F-box genes are located in sub-telomeric and heterochromatic chromosomal region in apple (2010)
  • Author(s): Sanhong Wang, Hiroyuki Kakui, Shinji Kikuchi, Takato Koba
  • Organizer: XXI International Congress on Sexual Plant Reproduction
  • Place of Presentation: イギリス・ブリストル大学
• [Remarks]
  • URL: http://www.h.chiba-u.jp/iden/
• [Remarks]
  • URL: http://www.h.chiba-u.jp/iden/