| Project/Area Number |
22770078
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | Chiba University |
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Principal Investigator |
KIKUCHI Shinji 千葉大学, 大学院・園芸学研究科, 助教 (80457168)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
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| Keywords | トレニア / 染色体 / 種分化 / SSR マーカー / 連鎖地図 / FISH / 遺伝資源 / SSRマーカー |
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| Research Abstract |
To identify an extra chromosome observed in interspecific hybrid between Torenia fournieri (2n=2x=18) and Torenia baillonii (2n=2x=16) at DNA level, 169 SSR markers were developed using fosmid and cDNA clone sequences and a partial linkage map was constructed in F_2 population of T. fournieri and T. violacea. Next, fosmid-FISH analysis has been conducted to screen fosmid clones derived from the extra chromosome, but the FISH signals of 19 clones tested did not formed on the extra chromosome. In addition, phylogenic analysis including other 7 Torenia species collected in south Asia suggests a hypothesis that T. baillonii lost the extra chromosome during the speciation.
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