| Project/Area Number |
22770087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biodiversity/Systematics
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| Research Institution | University of the Ryukyus |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2011: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2010: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
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| Keywords | 分子系統地理 / イモリ属 / 日本 / 遺伝的分化 / 種分化 / マイクロサテライト / ｍｔDNA / 東アジア / 系統解析 / 系統地理解析 / 集団遺伝解析 / 氷期 / ミトコンドリアDNA |
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| Research Abstract |
We investigated the phylogenetic relationships and estimated the historical demography of the Japanese fire-bellied newt, Cynops pyrrhogaster, from Japanese mainlands using 1407-bp sequences of the mitochondrial DNA (NADH6, tRNAglu, cyt b) and 1208-bp sequences of nuclear DNA (Rag-1) genes. Phylogenetic trees based on mitochondrial DNA revealed four major clades (NORTHERN, CENTRAL, WESTERN, and SOUTHERN clades) within this species. Nuclear gene presented no obvious patterns of geographic structure except for the NORTHERN clade, suggesting results of incomplete lineage sorting. We developed 15 novel microsatellite markers for C. pyrrhogaster using next generation sequencer. Genetic survey using these markers around a boundary region revealed allele frequencies of CENTRAL clade differ from those of WESTERN clade, suggesting these clades reproductively isolated from each other.
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