Exploring parasitic protists in animals inhabiting deep-sea methane seeps

• Project/Area Number: 22770091
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: TAKISHITA Kiyotaka 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 主任研究員 (90392951)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2010: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 深海 / 原生生物 / 寄生 / 進化 / 多様性

Research Abstract

Utilizing PCR techniques, the SSU rRNA gene from a novel alveolate was detected from gill tissues of the bivalves alyptogena spp. And Bathymodiolus spp. All of which harbor chemosynthetic bacteria and dominate methane seeps in Sagami Bay. In addition, the protist detected with PCR was localized within bacteriocytes of the bivalves with whole-mount fluorescence in situ hybridization.

Research Products

• [Journal Article] A novel alveolate in bivalves with chemosynthetic bacteria inhabiting deep-sea methane seeps (2013)
  • Author(s): Fumiya Noguchi, Masaru Kawato, Takao Yoshida, Yoshihiro Fujiwara, Katsunori Fujikura, Kiyotaka Takishita
  • Journal Title: Journal of Eukaryotic Microbiology Volume: Vol.60 Issue: 2 Pages: 158-165
  • DOI: 10.1111/jeu.12017
  • Peer Reviewed
• [Journal Article] Multigene phylogenies of diverse Carpediemonas-like organisms identify the closest relatives of 'amitochndriate' diplomonads and retortamonads (2012)
  • Author(s): Takishita K, Kolisko M, Komatsuzaki H, Yabuki A, Inagaki Y, Cepicka I, Smejkalova P, Silberman JD, Hashimoto T, Roger AJ, Simpson AGB.
  • Journal Title: Protist Volume: 163 Issue: 3 Pages: 344-355
  • DOI: 10.1016/j.protis.2011.12.007
  • Peer Reviewed
• [Journal Article] Evolution of elongation factor-like (EFL) protein in Rhizaria is revised by radiolarian EFL gene Sequences. (2012)
  • Author(s): Ishitani Y., Kamikawa R., Yabuki A., Tsutiya M., Inagaki Y. Takihita K.
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 59 Issue: 4 Pages: 367-373
  • DOI: 10.1111/j.1550-7408.2012.00626.x
  • Peer Reviewed
• [Journal Article] Algivore or phototroph? Plakobranchus ocellatus (Gastropoda) continuously acquires kleptoplasts and nutrition from multiple algal species in nature (2012)
  • Author(s): Taro Maeda, Euichi Hirose, Yoshito Chikaraishi, Masaru Kawato, Kiyotaka Takishita, Takao Yoshida, Heroen Verbruggen, Jiro Tanaka, Shigeru Shimamura, Yoshihiro Takaki, Masashi Tsuchiya, Kenji Iwai, Tadashi Maruyama
  • Journal Title: PLoS ONE Volume: Vol.7 Issue: 7 Pages: e42024-e42024
  • DOI: 10.1371/journal.pone.0042024
  • Peer Reviewed
• [Journal Article] Lateral transfer of tetrahymanol-synthesizing genes has allowed multiple diverse eukaryote lineages to independent adapt to environments without oxygen (2011)
  • Author(s): Takishita K., Chikaraishi Y., Leger MM., Kim E., Yabuki A., Ohkouchi N., Roger AJ.
  • Journal Title: Biology Direct Volume: 7 Issue: 1 Pages: 5-5
  • DOI: 10.1186/1745-6150-7-5
  • Peer Reviewed
• [Journal Article] Multigene phylogenetic analyses including diverse radiolarian species support the "Retaria" hypothesis-the sister relationship of Radiolaria and foraminifera (2011)
  • Author(s): Ishitani, Y.
  • Journal Title: Marine Micropaleontology Volume: 81 Issue: 1-2 Pages: 32-42
  • DOI: 10.1016/j.marmicro.2011.06.007
  • Peer Reviewed
• [Journal Article] Cyanobacterial endosymbionts in the benthic dinoflagellate Sinophysis canaliculata (Dinophyceae, Dinophysiales) (2011)
  • Author(s): Laura Escalera, Beatriz Reguera, Kiyotaka Takishita, Sadaaki Yoshimatsu, Kanae Koike, Kazuhiko Koike
  • Journal Title: Protist Volume: Vol.162 Issue: 2 Pages: 304-314
  • DOI: 10.1016/j.protis.2010.07.003
  • Peer Reviewed
• [Journal Article] Green-colored plastids in the dinoflagellate genus Lepidodinium are of core chlorophyte origin. (2011)
  • Author(s): Matsumoto T, 他5名, Nakayama T, Inouye I, HashimotoT & Inagaki Y.
  • Journal Title: Protist Volume: 162 Issue: 2 Pages: 268-276
  • DOI: 10.1016/j.protis.2010.07.001
  • Peer Reviewed
• [Journal Article] Green-colored plastids in the dinoflagellate genus Lepidodinium are of core chlorophyte origin (2011)
  • Author(s): Takuya Matsumoto
  • Journal Title: Protist Volume: 162 Pages: 268-276
  • Peer Reviewed
• [Journal Article] Cyanobacterial endosymbionts in the benthic dinoflagellate Sinophysis canaliculata (Dinophysiales, Dinophyceae) (2011)
  • Author(s): Laura Escalera
  • Journal Title: Protist Volume: 162 Pages: 304-314
  • Peer Reviewed
• [Journal Article] Molecular evidence that phylogenetically diverged ciliates are active in microbial mats of deep-sea cold seep sediment (2010)
  • Author(s): Kiyotaka Takishita
  • Journal Title: Journal of Eukaryotic Microbiology Volume: 57 Pages: 76-86
  • Peer Reviewed
• [Journal Article] Host-virus specificities of morvilliviruses predicted by structural modeling of marine mammal SLAM (CD150), a cellular receptor (2010)
  • Author(s): Kazue Ohishi
  • Journal Title: Comparative Immunology, Microbiology and Infectious Diseases Volume: 33 Pages: 227-241
  • Peer Reviewed
• [Journal Article] Cetacean Toll-like receptor 4 and myeloid differentiation factor 2 and possible cetacean-specific responses against Gram-negative bacteria (2010)
  • Author(s): Reiko Shishido
  • Journal Title: Comparative Immunology, Microbiology and Infectious Diseases Volume: 33
  • Peer Reviewed
• [Journal Article] A wide diversity of previously undetected free-living relatives of diplomonads isolated from marine/saline habitats (2010)
  • Author(s): Martin Kolisko
  • Journal Title: Environmental Microbiology Volume: 12 Pages: 2700-2710
  • Peer Reviewed
• [Journal Article] A phylogenetic mosaic plastid proteome and unusual plastid-targeting signals in the green-colored dinoflagellate Lepidodinium chlorophorum (2010)
  • Author(s): Marianne Minge
  • Journal Title: BMC Evolutionary Biology Volume: 10 Pages: 191-191
  • Peer Reviewed
• [Presentation] 放散虫と有孔虫を姉妹群とする「レタリア仮説」に基づいたアクチン,チューブリンおよびペプチド伸長因子の複雑な進化シナリオ (2012)
  • Author(s): 石谷佳之,石川奏太,神川龍馬,矢吹彬憲,土屋正史,稲垣祐司,瀧下清貴
  • Organizer: 日本地球惑星科学連合2012年大会
  • Place of Presentation: 幕張メッセ
  • Year and Date: 2012-05-24
• [Presentation] A novel alveolate in gill epithelium cells of bivalves with chemosynthetic bacteria inhabiting deep-sea methane seeps in Sagami Bay, Japan (2012)
  • Author(s): Fumiya Noguchi, Masaru Kawato, Takao Yoshida, Yoshihiro Fujiwara, Katsunori Fujikura, Kiyotaka Takishita
  • Organizer: Protist2012
  • Place of Presentation: Oslo University, Norway
• [Presentation] Fine structure of Telonema subtile Griessmann, 1913: A unique cytoskeletal structure among eukaryotes (2012)
  • Author(s): Akinori Yabuki, Wenche Eikrem, Kiyotaka Takishita
  • Organizer: Protist2012
  • Place of Presentation: Oslo University, Norway
• [Presentation] 放散虫と有孔虫が互いに姉妹群とする「レタリア仮説」に基づいたアクチン，チューブリンおよびペプチ ド伸長因子の複雑な進化シナリオ (2012)
  • Author(s): 石谷佳之
  • Organizer: 日本地球惑星科学連合2012年大会
  • Place of Presentation: 幕張メッセ国際会議場
• [Presentation] Fine structure of Telonema subtilis Griessmann, 1913: A flagellate with a unique cytoskeletal structure among eukaryotes (2012)
  • Author(s): Akinori Yabuki
  • Organizer: Protist2012
  • Place of Presentation: オスロ大学（ノルウェー）
• [Presentation] Evolutionary analysis of mitochondrial ancestry of diplomonads: Phylogenomics of mitochondrialrelated organelles in Carpediemonas-like organisms (2012)
  • Author(s): Martin Kolisko
  • Organizer: Protist2012
  • Place of Presentation: オスロ大学（ノルウェー）
• [Presentation] 相模湾メタン湧出活域に生息するヘイトウシンカイヒバリガイから検出された原生生物 (2011)
  • Author(s): 野口文哉,河戸勝,吉田尊雄,藤原義弘,藤倉克則,瀧下清貴
  • Organizer: 日本進化原生生物学研究会
  • Place of Presentation: 富山大学
  • Year and Date: 2011-06-11
• [Presentation] Multigene phylogenies of diverse Carpediemonas-like organisms identify the closest relatives of 'amitochondriate' diplomonads and retortamonads (2010)
  • Author(s): Kiyotaka Takishita
  • Organizer: 18th Meeting of the International Society for Evolutionary Protistology
  • Place of Presentation: 石川県立美術館(金沢市)
  • Year and Date: 2010-07-04
• [Presentation] Evolutionary analysis of mitochondrial ancestry of diplomonads: Phylogenomoics of mitochondrial-related organelles in Carpediemonas-like organism (2010)
  • Author(s): Martin Kolisko, Ryoma Kamikawa, Kiyotaka Takishita, Ivan Cepicka, Qianqian Zhang, Tetsuo Hashimoto, Akinori Yabuki, Yuji Inagaki, Andrew J. Roger, Alastair G.B. Simpson
  • Organizer: Protist2012
  • Place of Presentation: Oslo University, Norway