Diversity of bacteriophages infecting chemolithoautotrophs in hydrothermal ecosystems

• Project/Area Number: 22770092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biodiversity/Systematics
• Research Institution: Japan Agency for Marine-Earth Science and Technology
• Principal Investigator: YOSHIDA Yukari 独立行政法人海洋研究開発機構, 海洋・極限環境生物圏領域, 技術研究副主任 (10553216)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000); Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2010: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
• Keywords: 深海熱水噴出域 / バクテリオファージ / 化学合成独立栄養細菌 / 多様性 / 共進化 / 分子系統解析 / 化学合成細菌

Research Abstract

This main objective of this study is to reveal the interaction between chemolithoautotrophs and their phages in deep-sea hydrothermal vent ecosystems. We isolated a novel temperate phage that infected a deep-sea vent chemolithoautotrophic isolate of Epsilonproteobacteria, and its entire genome sequence was obtained and analyzed. The widespread occurrence of phage genes in diverse Epsilonproteobacteria supports early co-evolution between temperate phages and Epsilonproteobacteria prior to the divergence of their habitats and physiological adaptation.

Research Products

• [Journal Article] Genome sequence of a novel deep-sea vent epsilonproteobacterial phage provides new insight into the co-evolution of Epsilonproteobacteria and their phages (2013)
  • Author(s): Yoshida-Takashima Y., Takaki Y., Shimamura S., Nunoura T. and K. Takai
  • Journal Title: Extremophiles Volume: 17 Issue: 3 Pages: 405-419
  • DOI: 10.1007/s00792-013-0529-5
  • Peer Reviewed
• [Journal Article] Spatial distribution of viruses associated with planktonic and attached microbial communities in hydrothermal environments. (2012)
  • Author(s): Yoshida-Takashima, Y.
  • Journal Title: Appl. Environ. Microbiol. Volume: 78 Issue: 5 Pages: 1311-1320
  • DOI: 10.1128/aem.06491-11
  • Peer Reviewed
• [Presentation] 深海熱水活動域に生息するイプシロンプロテオバクテリアの溶原性ファージのゲノム解析 (2013)
  • Author(s): 吉田(高島)ゆかり、高木善弘、島村繁、布浦拓郎、高井研
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学
  • Year and Date: 2013-03-09
• [Presentation] 深海底熱水活動域に生息する化学合成独立栄養細菌に感染するファージのゲノム解析 (2012)
  • Author(s): 吉田(高島)ゆかり、高木善弘、島村繁、布浦拓郎、高井研
  • Organizer: 日本進化学会第14回東京大会
  • Place of Presentation: 首都大学東京(招待講演)
  • Year and Date: 2012-08-21
• [Presentation] Spatial distribution of viruses associated with planktonic and adhesive microbial communities in hydrothermal environments (2011)
  • Author(s): Yoshida-Takashima, Y., Nunoura T., Kazama H., Noguchi T., Inoue K., Akashi H., Yamanaka T., Toki T., Yamamoto H., Furushima Y., Ueno Y., Yamamoto H., Takai K
  • Organizer: The 6_<th> Aquatic Virus Workshop
  • Place of Presentation: オランダ海洋研究所. オランダ
  • Year and Date: 2011-11-03
• [Presentation] Spatial distribution of viruses associated with microbes in hydrothermal environments (2011)
  • Author(s): Yukari Yoshida-Takashima
  • Organizer: Aquatic Virus Workshop 6
  • Place of Presentation: オランダ国立海洋研究所(オランダ)
  • Year and Date: 2011-11-03
• [Presentation] 深海底熱水噴出域から単離した化学合成独立栄養細菌の溶原性ファージの性状解析 (2011)
  • Author(s): 吉田(高島)ゆかり、布浦拓郎、島村繁、高井研
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学
  • Year and Date: 2011-10-08
• [Presentation] 熱水噴出域におけるウイルスの分布と現存量 (2010)
  • Author(s): 吉田(高島)ゆかり、布浦拓郎、高井研
  • Organizer: 第26回日本微生物生態学会大会
  • Place of Presentation: 筑波大学
  • Year and Date: 2010-11-25
• [Presentation] 深海熱水活動域に生息するイプシロンプロテオバクテリアの溶原性ファージのゲノム解析
  • Author(s): 吉田(高島)ゆかり
  • Organizer: 第7回日本ゲノム微生物学会年会
  • Place of Presentation: 長浜バイオ大学
• [Presentation] 深海底熱水活動域に生息する化学合成独立栄養細菌に感染するファージのゲノム解析
  • Author(s): 吉田(高島)ゆかり
  • Organizer: 日本進化学会第14回東京大会
  • Place of Presentation: 首都大学東京
  • Invited
• [Remarks] アウトリーチ活動:施設一般公開における研究紹介
  • URL: http://www.jamstec.go.jp/biogeos/j/xbr/index.html