| Project/Area Number |
22770096
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | Ibaraki University |
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Principal Investigator |
UNNO Masaki 茨城大学, フロンティア応用原子科学研究センター, 准教授 (10359549)
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| Research Collaborator |
FUKUYAMA Keiichi 大阪大学, 理学研究科, 教授 (80032283)
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 酵素 / フィコシアノビリン / PcyA / 中性子結晶解析 / 水素原子 / 反応機構 / 大型結晶 / 宇宙実験 / 高分解能 / 中性子結晶構造解析 / ビリン還元酵素 / シアノバクテリア / 結晶成長 |
|---|
| Research Abstract |
We succeeded obtaining a big crystal of PcyA, a ferredoxin-dependent bilin reductase, which biosynthesizes an important pigment pytochromobilin. The crystal diffracted neutron up to 2.6 A resolution. If we can obtain bigger crystal than this one, we will able to structural analysis beyond 2 A resolution, and determine the position of hydrogen atoms in the structure. The reaction mechanism will be elucidated, soon.
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