X-ray crystal structure determination of the Iron/Cadmium transporter in plant.

• Project/Area Number: 22770097
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: MIYAZONO Kenichi 東京大学, 大学院・農学生命科学研究科, 特任助教 (90554486)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 蛋白質 / 植物 / 環境 / X線結晶構造解析

Research Abstract

To reveal the Iron/Cadmium transport mechanisms of two plant membrane protein, AtNRAMP3 and AtNRAMP4, which are thought to be key proteins for the cadmium toxicity in plant, we tried to determine the crystal structure of these proteins. We have succeeded overexpression of these membrane proteins using E. coli protein over expression system, but we couldn' t obtain the crystals of these proteins.