Structural analysis of H+-translocating inorganic pyrophosphatase

• Project/Area Number: 22770098
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: MIMURA Hisatoshi 東京大学, 分子細胞生物学研究所, 助教 (30463904)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2011: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000); Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: X線結晶解析 / 膜タンパク質 / 膜輸送体 / プロトンポンプ / 高等植物 / 結晶化

Research Abstract

Proton-translocating inorganic pyrophosphatase is an electrogenic proton pump that uses inorganic pyrophosphate as the substrate. The members of this protein family are widely distributed in most plants, but only some protozoa, bacteria, and archaebacteria. Although its importance in membrane transport systems, its coupling mechanism between the pyrophosphate hydrolysis and the active proton transport is still unknown. Here, structural study of the plant-derived enzyme was carried out.