Structural basis for specific cleavage of Lys63-linked polyubiquitin chains by Trabid

• Project/Area Number: 22770099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Structural biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SATO Yusuke 東京大学, 放射光連携研究機構, 助教 (50568061)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: X線結晶解析 / X線結晶構造解析 / ユビキチン / NF-κB

Research Abstract

We prepared the mouse, zebrafish and fly Trabid OTU domain and screened against 500 crystallization conditions. However, the OTU domains of Trabid were not crystallized. On the other hand, we determined the crystal structure of the HOIL-1L NZF domain in complex with linear di-ubiquitin. Together with structure based mutagenesis experiments our structure reveals the mechanism for specific recognition of linear chains by the HOL-1L NZF domain. This result was published in PNAS

Research Products

• [Journal Article] Specific recognition of linear ubiquitin chains by the Npl4 zinc finger(NZF) domain of the HOIL-1L subunit of the linear ubiquitin chain assembly complex (2011)
  • Author(s): Sato, Y., Fujita, H., Yoshikawa, A., Yamashita, M., Yamagata, A., Kaiser, S. E., Iwai, K., Fukai, S
  • Journal Title: Proceedings of National Academy of Science United States of America Volume: 108巻 Issue: 51 Pages: 20520-20525
  • DOI: 10.1073/pnas.1109088108
  • Peer Reviewed
• [Presentation] Structural basis for specific recognition of K63-linked polyubiquitin chains by TAB2 and TAB3 (2010)
  • Author(s): 佐藤裕介
  • Organizer: Pacifichem 2010
  • Place of Presentation: Hawaii Convention Center
  • Year and Date: 2010-12-19
• [Presentation] Structural basis for specific recognition of K63-linked polyubiquitin chains by TAB2 and TAB3 (2010)
  • Author(s): 佐藤裕介、吉川梓、山下雅美、山形敦史、深井周也
  • Organizer: Pacifichem 2010
  • Place of Presentation: Hawaii Convention Center USA
• [Remarks]
  • URL: http://www.iam.u-tokyo.ac.jp/srro/SRROLifeSciDivJp2/Top.html
• [Remarks]
  • URL: http://www.rcsb.org/pdb/explore/explore.do?structureId=3B0A
• [Remarks]
  • URL: http://www.rcsb.org/pdb/explore/explore.do?structureId=3B08