| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
|
|---|
| Research Abstract |
We succeeded the expression and purification of the urea transporter from Desulfovibrio vulgaris. After the optimization of the detergent, we obtained the crystals using the decyl maltoside. We collected the low resolution diffraction dataset on this crystal. We expressed the mouse urea transporter as the GFP fused form. FSEC analysis revealed that the mouse urea transporter exists as the monodisperse state, suitable for the structural analysis.
|
