Structural analysis for the urea transporter
| Project/Area Number |
22770100
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Structural biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
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| Project Status |
Completed (Fiscal Year 2011)
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| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | X線結晶解析 / タンパク質 / 構造生物学 |
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| Research Abstract |
We succeeded the expression and purification of the urea transporter from Desulfovibrio vulgaris. After the optimization of the detergent, we obtained the crystals using the decyl maltoside. We collected the low resolution diffraction dataset on this crystal. We expressed the mouse urea transporter as the GFP fused form. FSEC analysis revealed that the mouse urea transporter exists as the monodisperse state, suitable for the structural analysis.
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Report
(3 results)
Research Products
(7 results)
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[Journal Article] Specific recognition of linear ubiquitin chains by the Npl4 zinc finger(NZF) domain of the HOIL-1L subunit of the linear ubiquitin chain assembly complex2011
Author(s)
Sato, Y., Fujita, H., Yoshikawa, A., Yamashita, M., Yamagata, A., Kaiser, S. E., Iwai, K., Fukai, S
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Journal Title
Proceedings of National Academy of Science United States of America
Volume: 108巻
Issue: 51
Pages: 20520-20525
DOI
Related Report
Peer Reviewed
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