Structural study of translesion DNA polymeraseζ/REV1 complex
Project/Area Number |
22770109
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Yokohama City University |
Principal Investigator |
HASHIMOTO Hiroshi 横浜市立大学, 生命ナノシステム科学研究科, 助教 (40336590)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | X線結晶解析 / DNA複製 / DNA損傷 / DNAポリメラーゼ / 損傷乘り越えDNA合成 / 突然変異 / 構造解析 / タンパク質間相互作用 / 損傷乗り越えDNA合成 |
Research Abstract |
REV1, REV3 and REV7 are pivotal proteins in translesion DNA synthesis that allows to continue DNA synthesis even in the presence of DNA damage. REV1 and REV3 are error-prone DNA polymerases. REV7 interacts with both polymerases, thereby acts as an adaptor protein that functionally links those polymerases. The ternary complex of the C-terminal domain of human REV1 in complex with REV7 bound to a REV3 fragment was successfully crystallized. The crystals belong to the space group of P3_121 with the cell-dimension of a=b=74.7, c=124.5 Å and・=120°. Electron density map obtained from molecular replacement method using REV7-REV3 complex as a search model clearly shows that the REV1 C-terminal domain adopts a four-helices bundle structure.
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Report
(3 results)
Research Products
(50 results)
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[Journal Article] Mechanistic insights into the activation of Rad51-mediated strand exchange from the structure of a recombination activator, the Swi5-Sfr1 complex2013
Author(s)
N.Kuwabara, Y.Murayama, H.Hashimoto, Y.Kokabu, M.Ikeguchi, M.Sato, K.Mayanagi, Y.Tsutsui, H.Iwasaki,and T.Shimizu
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Journal Title
Structure
Volume: 20
Issue: 3
Pages: 440-449
DOI
Related Report
Peer Reviewed
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[Journal Article] Identification of a small molecule PCNA inhibitor that disrupts interactions with PIP-Box proteins and inhibits DNA replication2012
Author(s)
Chandanamali Punchihewa, Akira Inoue, Asami Hishiki, Yoshihiro Fujikawa, Michele Connelly, Benjamin Evison, Youming Shao, Richard Heath, Isao Kuraoka, Patrick Rodrigues, Hiroshi Hashimoto, Masanobu Kawanishi, Mamoru Sato, Takashi Yagi, Naoaki Fujii
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Journal Title
J Biol Chem
Volume: 287
Issue: 17
Pages: 14289-14300
DOI
Related Report
Peer Reviewed
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[Journal Article] The Development of N-α-(2-Carboxyl)benzoyl-N5-(2-fluoro-1-iminoethyl)-L-The Development of N-α-(2-Carboxyl)benzoyl-N5-(2-fluoro-1-iminoethyl)-L-ornithine Amide (o-F-amidine) and N-α-(2-Carboxyl)benzoyl-N5-(2-chloro-1-iminoethyl)-L-ornithine Amide (o-Cl-amidine) As Second Generation Protein Arginine Deiminase (PAD) Inhibitors2011
Author(s)
C.P.Causey, J.E.Jones, J.L.Slack, D.Kamei, L.E.Jones, Jr., V.Subramanian, B.Knuckley, P.Ebrahimi, A.A.Chumanevich, Y.Luo, H.Hashimoto, M.Sato, L.J.Hofseth P.R.Thompson
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Journal Title
J.Med.Chem.
Volume: 54
Issue: 19
Pages: 6919-6935
DOI
Related Report
Peer Reviewed
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[Journal Article] Fission yeast Swi5-Sfr1 protein complex, an activator of Rad51 recombinase, forms an extremely elongated dogleg-shaped structure2011
Author(s)
Kokabu Y, Murayama Y, Kuwabara N, Oroguchi T, Hashimoto H, Tsutsui Y, Nozaki N, Akashi S, Unzai S, Shimizu T, Iwasaki H, Sato M, Ikeguchi M
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Journal Title
J Biol Chem
Volume: 286
Issue: 50
Pages: 43569-43576
DOI
Related Report
Peer Reviewed
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[Journal Article] Structure and functional characterization of Vibrio parahaemolyticus thermostable direct hemolysin2010
Author(s)
Itaru Yanagihara, Kumiko Nakahira, Tsutomu Yamane, Shuji Kaieda, Kouta Mayanagi, Daizo Hamada, Takashi Fukui, Kiyouhisa Ohnishi, Shin'ichiro Kajiyama, Toshiyuki Shimizu, Mamoru Sato, Takahisa Ikegami, Mitsunori Ikeguchi, Takeshi Honda, Hiroshi Hashimoto
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Journal Title
J Biol Chem
Volume: 285
Pages: 12299-12307
Related Report
Peer Reviewed
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[Presentation] Structure of REV7 in complex with a REV3 fragment and structural implication of the interaction between DNA polymeraseζ and REV12011
Author(s)
Hiroshi Hashimoto, Kodai Hara, Yoshiki Murakumo, Shunsuke Kobayashi, Toshiaki Kogame, Satoru Unzai, Satoko Akashi, Shunichi Takeda, Toshiyuki Shimizu, Mamoru Sato
Organizer
NH Grand Hotel Krasnapolsky
Place of Presentation
Amsterdam, Netherlands
Related Report
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[Presentation] Crystal structure of zinc-finger domain of Nanos and its functional implications2011
Author(s)
Hiroshi Hashimoto, Kodai Hara, Asami Hishiki, Shigeta Kawaguchi, Takuya Yoshizawa, Satoru Unzai, Yutaka Tamaru, Toshiyuki Shimizu, Mamoru Sato
Organizer
XXII Congress IUCr
Place of Presentation
Municipal Conference Center, Madrid, Spain
Related Report
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[Presentation] Structure of REV7 in complex with a REV3 fragment and structural implication of the interaction between DNA polymerase ζ and REV12011
Author(s)
Hiroshi Hashimoto, Kodai Hara, Yoshiki Murakumo, Shunsuke Kobayashi, Toshiaki Kogame, Satoru Unzai, Satoko Akashi, Shunichi Takeda, Toshiyuki Shimizu, Mamoru Sato
Organizer
Current Opinion in Structural Biology & DNA Repair
Place of Presentation
NH Grand Hotel Krasnapolsky, Amsterdam, Netherlands
Related Report
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[Presentation] Crystal structure of thermostable direct hemolysin from Vibrio parahaemolyticus2010
Author(s)
H.Hashimoto, K.Nakahira, T.Yamane, T.Fukui, K.Ohnishi, T.Shimizu, T.Honda, M.Sato, M.Ikeguchi, I.Yanagihara
Organizer
AsCA2010
Place of Presentation
Busan, Korea
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