Structural analysis of transcription factor TFIIH subcomplex
Project/Area Number |
22770110
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Structural biochemistry
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Research Institution | Kagawa University (2013) University of Hyogo (2010-2012) |
Principal Investigator |
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Project Period (FY) |
2010-04-01 – 2014-03-31
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Project Status |
Completed (Fiscal Year 2013)
|
Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2011: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
|
Keywords | タンパク質 / X線結晶構造解析 / 結晶 / 転写因子 / タンパク質の精製 / タンパク質複合体 / X線結晶解析 / TFIIH / TFIIE |
Research Abstract |
The purpose of this study is to determine the three-dimensional structures of transcription factors by X-ray crystallographic analysis, in order to understand the molecular mechanism of the DNA transcriptional regulation with RNA polymerase II. Transcription factors function as a complex of various kinds of proteins. To prepare the sample suitable for crystallization, the purification of the significant protein complex involved in transcriptional regulation was performed by using a co-expression system of E. coli and insect cells.
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Report
(5 results)
Research Products
(35 results)
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[Journal Article] Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from B. subtilis2012
Author(s)
Y. Shomura, E. Hinokuchi, H. Ikeda, A. Senoo, Y. Takahashi, J. Saito, H. Komori, N. Shibata, Y. Yonetani, & Y. Higuchi
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Journal Title
Protein Sci
Volume: 21
Pages: 707-716
Related Report
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[Journal Article] Structural and enzymatic characterization of BacD, an L-amino acid dipeptide ligase from Bacillus subtilis.2012
Author(s)
Shomura Y, Hinokuchi E, Ikeda H, Senoo A, Takahashi Y, Saito J, Komori H, Shibata N, Yonetani Y, Higuchi Y.
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Journal Title
Protein Sci.
Volume: 21
Pages: 707-716
Related Report
Peer Reviewed
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[Journal Article] Crystal structures of ethanolamine ammonia-lyase complexed with coenzyme B12 analogs and substrates2010
Author(s)
N. Shibata, H. Tamagaki, S. Ohtsuki, N. Hieda, K. Akita, H. Komori, Y. Shomura, S. Terawaki, K. Mori, N. Yasuoka, Y. Higuchi & T. Toraya
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Journal Title
J. Biol. Chem
Volume: 285
Pages: 26484-26493
Related Report
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[Journal Article] Cytochrome c polymerization by successive domain swapping at the C-terminal helix2010
Author(s)
S. Hirota, Y. Hattori, S. Nagao, M. Taketa, H. Komori, H. Kamikubo, Z. Wang, I. Takahashi, S. Negi, Y. Sugiura, M. Kataoka & Y. Higuchi
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 107
Pages: 12854-12859
NAID
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