Mechanism of large protein secretion coordinated by the small GTPase and the ER-resident proteins

• Project/Area Number: 22770120
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: SAITO Kota 東京大学, 大学院・薬学系研究科, 助教 (60549632)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2010: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: 分泌 / コラーゲン / 小胞体 / COPII / 癌

Research Abstract

Collagen synthesized in the ER is too big to fit into conventional transport carriers and the mechanism how collagens export from the ER is still largely unclear. Here we show that cTAGE5 localizes to the ER exit sites and forms a complex with TANGO1, a previously characterized cargo receptor for collagen VII. cTAGE5 as well as TANGO1 is required for collagen VII secretion. We propose that cTAGE5 acts as a co-receptor of TANGO1 for collagen VII export from the ER.

Research Products

• [Journal Article] cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites (2011)
  • Author(s): Saito K, Yamashiro K, Ichikawa Y, ErlmannP, Kontani K, Malhotra V, Katada T
  • Journal Title: Mol. Biol. Cell Volume: 22 Pages: 2301-2308
• [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs (2011)
  • Author(s): Takahashi S, Sakurai K, Ebihara A, Kajiho H, Saito K, Kontani K, Nishina H, Katada T.
  • Journal Title: Nucleic Acids Res Volume: 39 Pages: 3446-3457
• [Journal Article] cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites (2011)
  • Author(s): Saito, K., Yamashiro, K., Ichikawa, Y., Erlmann, P., Kontani, K., Malhotra, V., and Katada, T.
  • Journal Title: Mol Biol Cell Volume: 22 Issue: 13 Pages: 2301-2308
  • DOI: 10.1091/mbc.e11-02-0143
  • Peer Reviewed
• [Journal Article] RhoA activation participates in rearrangement of processing bodies and release of nucleated AU-rich mRNAs. (2011)
  • Author(s): Takahashi S, et al.
  • Journal Title: Nucleic Acids Res. Volume: 39(in press)(掲載確定)
  • Peer Reviewed
• [Journal Article] The LIM protein Ajuba is required for ciliogenesis and left-right axis determination in medaka. (2010)
  • Author(s): Nagai Y, et al.
  • Journal Title: Biochem.Biophys.Res.Commun. Volume: 396(4) Pages: 887-893
  • Peer Reviewed
• [Presentation] cTAGE5 mediates collagen secretion through interaction with TANGO1 at endoplasmic reticulum exit sites (2012)
  • Author(s): Kota Saito, Koh Yamashiro, Yuki Ichikawa, Noriko Shimazu, and Toshiaki Katada
  • Organizer: グローバルCOE
  • Place of Presentation: 大磯
  • Year and Date: 2012-02-04
• [Presentation] cTAGE5 is involved in the endoplasmic reticulum to golgi trafficking in mammalian cells. (2011)
  • Author(s): Saito K, et al.
  • Organizer: ASBMB Special Symposia, Biochemistry of Membrane Traffic : Secretory and Endocytic Pathways.
  • Place of Presentation: 米国/タホ
  • Year and Date: 2011-10-29
• [Presentation] 小胞体からのコラーゲン分泌に関与するcTAGE5/TANGO1複合体の機能解析、ポスター発表 (2011)
  • Author(s): 齋藤康太、山城昂、市川由希、嶋津典子、堅田利明
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 小胞体からのコラーゲン分泌に関与するcTAGE5/TANGO1複合体の機能解析 (2011)
  • Author(s): 齋藤康太, 他
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 表皮水疱症及び肝線維化の病態理解に向けたコラーゲン分泌機構の解明 (2011)
  • Author(s): 齋藤康太、山城昂、市川由希、嶋津典子、堅田利明
  • Organizer: 新学術領域「細胞内ロジスティクス」班会議
  • Place of Presentation: 鳥羽
  • Year and Date: 2011-06-03
• [Presentation] cTAGE5はER exit siteにおいてTANGO1と協調してVII型コラーゲンの輸送を補助するポスター発表 (2011)
  • Author(s): 山城昂、市川由希、嶋津典子、堅田利明、齋藤康太
  • Organizer: 新学術領域「細胞内ロジスティクス」班会議
  • Place of Presentation: 鳥羽
  • Year and Date: 2011-06-03
• [Presentation] cTAGE5はERexit siteにおいてTANGO1と協調してVII型コラーゲンの輸送を補助する(ポスター発表) (2010)
  • Author(s): 山城昂,市川由希,齋藤康太,堅田利明
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-07
• [Presentation] cTAGE5 is involved in the endoplasmic reticulum to golgi trafficking in mammalian cells. ASBMB(American Society for Biochemistry and Molecular Biology) Special Symposia, Biochemistry of Membrane Traffic (2010)
  • Author(s): Saito K, Yamashiro K, Ichikawa Y, ErlmannP, Malhotra V, Katada T.
  • Organizer: Secretoryand Endocytic Pathways
  • Place of Presentation: Tahoe City, USA
  • Year and Date: 2010-10-29
• [Remarks] 研究室ホームページ
  • URL: http://www.f.u-tokyo.ac.jp/~seiri