Mechanisms of COPII vesicle formation at ER exit sites

• Project/Area Number: 22770121
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: The University of Tokyo
• Principal Investigator: YORIMITSU Tomohiro 東京大学, 大学院・総合文化研究科, 助教 (00534364)
• Project Period (FY): 2010 – 2012
• Project Status: Completed (Fiscal Year 2012)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 小胞体 / 小胞輸送 / COPII 小胞 / ERES / COPII小胞

Research Abstract

COPII vesicles are formed at the ER exit sites, which mediate the ER-to-Golgi traffic. Sec16 acts as a key factor for ERES formation as well as COPII-mediated transport. Here we have shown that Sec16 is self-assembled to form a homooligomer on the membranes. In addition, we find that Sec16 negatively regulates activation of Sar1 GTP hydrolysis by COPII coat proteins.

Research Products

• [Journal Article] COPII machinery cooperates with ER-localized Hsp40 to sequester misfolded membrane proteins into ER-associated compartment. (2013)
  • Author(s): Kakoi, S., Yorimitsu, T., Sato, K.
  • Journal Title: Mol Biol Cell Volume: 24 Issue: 5 Pages: 633-642
  • DOI: 10.1091/mbc.e12-08-0639
  • Peer Reviewed
• [Journal Article] Insight into structural and regulatory roles of Sec16 in COPII vesicle formation at ER exit sites (2012)
  • Author(s): T. Yorimitsu and K. Sato
  • Journal Title: Mol. Biol. Cell Volume: 23 Issue: 15 Pages: 2930-2942
  • DOI: 10.1091/mbc.e12-05-0356
  • Peer Reviewed
• [Journal Article] Involvement of the penta-EF-hand protein Pef1p in the Ca2+-dependent regulation of COPII subunit assembly in Saccharomyces cerevisiae (2012)
  • Author(s): Yoshibori, M., Yorimitsu, T., Sato, K.
  • Journal Title: PLoS ONE Volume: 7 Issue: 7 Pages: e40765-e40765
  • DOI: 10.1371/journal.pone.0040765
  • Peer Reviewed
• [Journal Article] Sed4p stimulates Sarlp GTP hydrolysis and promotes limited coat disassembly (2011)
  • Author(s): C. Kodera, T. Yorimitsu, A. Nakano, and K. Sato
  • Journal Title: Traffic Volume: 12 Issue: 5 Pages: 591-599
  • DOI: 10.1111/j.1600-0854.2011.01173.x
  • Peer Reviewed
• [Journal Article] Sed4p stimulates Sar1p GTP hydrolysis and promotes limited coat disassembly (2011)
  • Author(s): Chie Kodera, Tomohiro Yorimitsu, Akihiko Nakano, Ken Sato
  • Journal Title: Traffic Volume: 12 Pages: 591-599
  • Peer Reviewed
• [Presentation] ER exit sitesにおけるCOPII 小胞形成の制御メカニズム (2012)
  • Author(s): 依光 朋宏
  • Organizer: 遺伝研研究会
  • Place of Presentation: 三島
  • Year and Date: 2012-03-21
• [Presentation] ER exit sitesにおけるCOPII小胞形成の制御メカニズム (2012)
  • Author(s): 依光朋宏
  • Organizer: 遺伝研研究会
  • Place of Presentation: 遺伝学研究所(静岡県)(招待講演)
  • Year and Date: 2012-03-21
• [Presentation] Sec16 function in COPII vesicle formation at ER exit sites (2011)
  • Author(s): 依光 朋宏、佐藤 健
  • Organizer: 第83回日本生化学大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] Sec16 function in COPII vesicle formation at ER exit sites (2011)
  • Author(s): 依光朋宏
  • Organizer: 日本生化学会
  • Place of Presentation: 京都国際会館(京都府)
  • Year and Date: 2011-09-23