pproach for circadian oscillatory mechanisms based on phosphorylation rhythms of clock proteins
| Project/Area Number |
22770122
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Functional biochemistry
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Project Status |
Completed (Fiscal Year 2012)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2011: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2010: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Keywords | 脳・神経 / 動物行動 / サーカディアンリズム / 概日時計 / 時計タンパク質 / リン酸化シグナリング / ChIP-Seq / 次世代シーケンサー / RNA-Seq / DNA結合 / 転写制御 |
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| Research Abstract |
In the mammalian circadian clockwork, CLOCK and BMAL1 bind to DNA and play an important role in the transcriptional feedback loops. In this study, we performed ChIP-Seq analysis and determined all the CLOCK-binding regions in genome-wide manner. RNA-Seq analysis showed circadian profiles of all the transcripts. Furthermore, we identified JNK as novel protein kinase for phosphorylation of CLOCK-BMAL1 complex, and behavioral analysis of JNK3-KO mice showed that JNK is a key player in the photic regulation of circadian clockwork.
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Report
(4 results)
Research Products
(57 results)
