Cellular role and regulatory mechanism for a novel deubiquitinating enzyme in the endoplasmic reticulum

• Project/Area Number: 22770123
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Tokyo Institute of Technology
• Principal Investigator: NAKAMURA Nobuhiro 東京工業大学, 大学院・生命理工学研究科, 准教授 (80361765)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 小胞体 / 脱ユビキチン化 / プロテアソーム分解 / 脱ユビキチン化酵素 / 小胞体関連分解 / 品質管理 / プロセシング / 分子シャペロン

Research Abstract

This study has demonstrated that 1) USP19, a transmembrane deubiquitinating enzyme in the endoplasmic reticulum(ER), deubiquitinates not only typical substrates of ER-associated degradation(ERAD), but also ERAD ubiquitinating enzymes, and 2) the enzymatic activity of USP19 is dependent on the domain structures and processing of USP19. These results provide better understand of the molecular mechanism for the regulation of the ERAD pathway as well as the USP19 activity.

Research Products

• [Journal Article] Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids (2012)
  • Author(s): Yogo, K., Tojima, H., Ohno, J. Y., Ogawa, T., Nakamura, N., Hirose, S., Takeya, T., & Kohsaka, T.
  • Journal Title: Histochem. Cell Biol Volume: 137 Pages: 53-65
  • NAID: 120003518501
  • Peer Reviewed
• [Journal Article] Identification of SAMT family proteins as substrates of MARCH11 in mouse spermatids (2012)
  • Author(s): Yogo, K., Tojima, H., Ohno, J.Y., Ogawa, T., Nakamura, N., ら(計8名)
  • Journal Title: Histochem.Cell Biol. Volume: 137 Issue: 1 Pages: 53-65
  • DOI: 10.1007/s00418-011-0887-y
  • NAID: 120003518501
  • Peer Reviewed
• [Journal Article] Membrane-associated RING-CH 10(MARCH10) is a microtubule-associated E3 ubiquitin ligase of the spermatid flagella (2011)
  • Author(s): Iyengar, P. V., Hirota, T., Hirose, S., & Nakamura, N.
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 39082-39090
  • Peer Reviewed
• [Journal Article] The role of the transmembrane RING finger proteins in cellular and organelle function (2011)
  • Author(s): Nakamura, N.
  • Journal Title: Membranes 1 Pages: 354-393
  • Peer Reviewed
• [Journal Article] Membrane-associated RING-CH 10 (MARCH10) is a microtubule-associated E3 ubiquitin ligase of the spermatid flagella (2011)
  • Author(s): Iyengar, P.V., Hirota,T., Hirose, S., Nakamura, N.
  • Journal Title: J.Biol.Chem. Volume: 286 Issue: 45 Pages: 39082-39090
  • DOI: 10.1074/jbc.m111.256875
  • Peer Reviewed
• [Journal Article] The role of the transmembrane RING finger proteins in cellular and organelle function (2011)
  • Author(s): Nakamura, N
  • Journal Title: Membranes Volume: 1 Issue: 4 Pages: 354-393
  • DOI: 10.3390/membranes1040354
  • Peer Reviewed
• [Journal Article] Identification of zebrafish FXYD11a protein that is highly expressed in ion-transporting epithelium of the gill and skin and its possible role in ion homeostasis (2010)
  • Author(s): Saito, K., Nakamura, N., Ito, Y., Hoshijima, K., Esaki, M. Zhao, B., & Hirose, S.
  • Journal Title: Front. Physio Volume: 1 Pages: 129-129
  • Peer Reviewed
• [Journal Article] Identification of zebrafish FXYD11a protein that is highly expressed in ion-transporting epithelium of the gill and skin and its possible role in ion homeostasis (2010)
  • Author(s): Kaori Saito, Nobuhiro Nakamura, ら(計7名)
  • Journal Title: Front.Physio. Volume: 129 Pages: 1-14
  • Peer Reviewed
• [Presentation] Regulation of expression of TEB4(MARCH6) ubiquitin ligase by USP19 deubiquitinating enzyme (2011)
  • Author(s): Nakamura, N. & Hirose, S.
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 札幌
  • Year and Date: 2011-06-27
• [Presentation] Regulation of expression of TEB4 (MARCH6) ubiquitin ligase by USP19 deubiquitinating enzyme (2011)
  • Author(s): Nakamura, N., Hirose, S.
  • Organizer: 第63回日本細胞生物学会大会
  • Place of Presentation: 北海道・札幌
  • Year and Date: 2011-06-27
• [Presentation] Regulation of mitochondrial morphology by ubiquitination (2011)
  • Author(s): Nakamura, N.
  • Organizer: 5th Conference of Korean Society for Mitochondrial Research and Medicine
  • Place of Presentation: Daegue, Korea
  • Year and Date: 2011-06-18
• [Presentation] Regulation of mitochondrial morphology by ubiquitination (2011)
  • Author(s): Nakamura, N.
  • Organizer: 5th Conference of Korean Society for Mitochondrial Research and Medicine
  • Place of Presentation: Daegue, Korea(招待講演)
  • Year and Date: 2011-06-18
• [Remarks]
  • URL: http://www.hirose.bio.titech.ac.jp/index.html
• [Remarks]
  • URL: http://www.hirose.bio.titech.ac.jp/
• [Remarks]
  • URL: http://www.hirose.bio.titech.ac.jp/