Nascent polypeptide chain that monitors membrane protein biogenesis in Bacullus subtilis.
Project/Area Number |
22770125
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Research Category |
Grant-in-Aid for Young Scientists (B)
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Allocation Type | Single-year Grants |
Research Field |
Functional biochemistry
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Research Institution | Kyoto Sangyo University |
Principal Investigator |
CHIBA Shinobu 京都産業大学, 総合生命科学部, 助教 (20523517)
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Project Period (FY) |
2010 – 2011
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Project Status |
Completed (Fiscal Year 2011)
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Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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Keywords | 翻訳アレスト / 膜蛋白質分解 / リボソーム / 蛋白質膜組込 / 枯草菌 / タンパク質膜組込 |
Research Abstract |
We constructed an in vitro translation system composed of each purified translation component and Bacillus subtilis ribosome. This system allowed us to reproduce elongation arrest of B. subtilis MifM in vitro. We also demonstrated that interactions between the ribosome and nascent polypeptide could be species-specific. We showed modularity of translation elongation arrest motif of MifM.
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Report
(3 results)
Research Products
(13 results)
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[Journal Article] Holin triggering in real time2011
Author(s)
White, R., 千葉志信, Pang, T., Dewey, J. S., Savva, C. G., Holzenburg, A., Pogliano, K. and Young, R
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Journal Title
Proc. Natl. Acad. Sci. USA
Volume: 108
Issue: 2
Pages: 798-803
DOI
Related Report
Peer Reviewed
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