Nascent polypeptide chain that monitors membrane protein biogenesis in Bacullus subtilis.

• Project/Area Number: 22770125
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Kyoto Sangyo University
• Principal Investigator: CHIBA Shinobu 京都産業大学, 総合生命科学部, 助教 (20523517)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 翻訳アレスト / 膜蛋白質分解 / リボソーム / 蛋白質膜組込 / 枯草菌 / タンパク質膜組込

Research Abstract

We constructed an in vitro translation system composed of each purified translation component and Bacillus subtilis ribosome. This system allowed us to reproduce elongation arrest of B. subtilis MifM in vitro. We also demonstrated that interactions between the ribosome and nascent polypeptide could be species-specific. We showed modularity of translation elongation arrest motif of MifM.

Research Products

• [Journal Article] Nascentome analysis uncovers futile protein synthesis in Escherichia coli (2012)
  • Author(s): Ito K., et al
  • Journal Title: PLoS ONE Volume: 6 Issue: 12 Pages: e28413-e28413
  • DOI: 10.1371/journal.pone.0028413
  • Peer Reviewed
• [Journal Article] Post liberation cleavage of signal peptides is catalyzed by the S2P protease in bacteria (2011)
  • Author(s): Saito, A., et al
  • Journal Title: Proc.Natl.Acad.Sci, USA Volume: 108 Issue: 33 Pages: 13740-13745
  • DOI: 10.1073/pnas.1108376108
  • Peer Reviewed
• [Journal Article] Recruitment of a species-specific arrest module to monitor different cellular processes (2011)
  • Author(s): Chiba, S., et al
  • Journal Title: Proc.Natl.Acad.Sci, USA Volume: 108 Issue: 15 Pages: 6073-6078
  • DOI: 10.1073/pnas.1018343108
  • Peer Reviewed
• [Journal Article] Holin triggering in real time (2011)
  • Author(s): White, R., 千葉志信, Pang, T., Dewey, J. S., Savva, C. G., Holzenburg, A., Pogliano, K. and Young, R
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 108 Issue: 2 Pages: 798-803
  • DOI: 10.1073/pnas.1011921108
  • Peer Reviewed
• [Presentation] 翻訳途上鎖MifMによるタンパク質膜組込のモニタリング (2012)
  • Author(s): 千葉志信
  • Organizer: 第1回Ribosome Meeting
  • Place of Presentation: 広島
  • Year and Date: 2012-03-15
• [Presentation] 翻訳途上鎖のダイナミズムによる翻訳伸長の制御とそれを利用した蛋白質局在化モニタリング機構 (2011)
  • Author(s): 千葉志信
  • Organizer: 第37回生体エネルギー研究会
  • Place of Presentation: 京都
  • Year and Date: 2011-12-21
• [Presentation] In vitro study of translation arrest of MifM, a regulatory nascent chain that monitors membrane protein biogenesis in Bacillus subtilis (2011)
  • Author(s): 千葉志信
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] In vitro study of translation arrest of MifM, a regulatory nascent chain that monitors membrane protein biogenesis in Bacillus subtilis (2011)
  • Author(s): 千葉志信
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜(招待講演)
  • Year and Date: 2011-12-15
• [Presentation] Modularity and species-specificity of translation arrest motifs in translocation monitor proteins (2011)
  • Author(s): 千葉志信
  • Organizer: Cold Spring Harbor Asia Conference
  • Place of Presentation: 蘇州(中国)
  • Year and Date: 2011-09-28
• [Presentation] タンパク質膜組込をモニターする枯草菌翻訳アレスト因子MifMのin vitro解析 (2011)
  • Author(s): 千葉志信
  • Organizer: グラム陽性菌ゲノム機能会議
  • Place of Presentation: 福山
  • Year and Date: 2011-08-26
• [Presentation] Visualizing dynamic "nascentome" of the cell (2010)
  • Author(s): 伊藤維昭
  • Organizer: International Symposium on Protein Community
  • Place of Presentation: 奈良
  • Year and Date: 2010-09-14
• [Presentation] 枯草菌翻訳アレスト因子mifMのin vitro翻訳系による解析 (2010)
  • Author(s): 千葉志信
  • Organizer: 平成22年度グラム陽性菌ゲノム会議
  • Place of Presentation: 南木曾
  • Year and Date: 2010-09-03
• [Presentation] Visualizing dynamic nascentome of the cell. (2010)
  • Author(s): 伊藤維昭
  • Organizer: FASEB Summer Research Conference Protein Folding in the Cell
  • Place of Presentation: Vermont (USA)
  • Year and Date: 2010-07-27