Research Project
Grant-in-Aid for Young Scientists (B)
Recycling endosomes in interphase are distributed throughout the cell. In contrast, in M phase, they are clustered around the centrosome. However, little is known about the functional behavior of recycling endosomes in M phase. The purpose of this study is to isolate recycling endosomes from mitotic cells and to identify characteristic proteins in isolated recycling endosomes. We have developed a novel tandem affinity tag purification method and purified recycling endosomes from cells synchronized in M phase.
All 2012 2011 2010 Other
All Journal Article (3 results) (of which Peer Reviewed: 3 results) Presentation (11 results) Book (2 results) Remarks (1 results)
EMBO J
Volume: 31 Issue: 11 Pages: 2590-2603
10.1038/emboj.2012.89
120004161052
The EMBO Journal
Volume: 31(掲載確定)
Molecular Biology of the Cell
Volume: 21 Pages: 2746-2755
http://www.pharm.kyoto.u.ac.jp/physchem/