Identification of novel kinases in Wnt signaling

• Project/Area Number: 22770127
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Osaka University
• Principal Investigator: SATO Akira 大阪大学, 大学院・医学系研究科, 助教 (70464302)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: Wntシグナル / リン酸化酵素 / Dvl / LRP5/ 6 / Wnt5a / Dishevelled(Dvl)

Research Abstract

Wnts control various cellular functions including proliferation, differentiation, death, migration, and polarity, by activating multiple intracellular signaling cascades including the.β-catenin-dependent and-independent pathways. Dishevelled(Dvl), which has multiple phosphorylation sites, is an essential intracellular component for both pathways. However, the detailed mechanisms and regulations of Wnt signaling cascades through Dvl have still been poorly understood. In this study, I performed siRNA library screenings to identify novel kinases that phosphorylate Dvl.

Research Products

• [Journal Article] Localization of glypican-4 in different membrane microdomains is involved in the regulation of Wnt signaling (2012)
  • Author(s): Sakane H., Yamamoto H., Matsumoto S., Sato A., and Kikuchi A.
  • Journal Title: J. Cell Sci Volume: 125(Pt 2) Pages: 449-460
  • Peer Reviewed
• [Journal Article] An anti-Wnt5a antibody suppresses metastasis of gastric cancer cells in vivo by inhibiting receptor-mediated endocytosis (2012)
  • Author(s): Hanaki H., Yamamoto H., Sakane H., Matsumoto S., Ohdan H., Sato A., Kikuchi A.
  • Journal Title: Mol. Cancer Ther Volume: 11 Pages: 298-307
  • Peer Reviewed
• [Journal Article] Localization of glypican-4 in different membrane microdomains is involved in the regulation of Wnt signaling (2012)
  • Author(s): Sakane, H., et.al.
  • Journal Title: J.Cell Sci. Volume: 125 Issue: 2 Pages: 449-460
  • DOI: 10.1242/jcs.091876
  • Peer Reviewed
• [Journal Article] An Wnt5a-antibody suppresses metastasis of gastric cancer cells in vivo by inhibiting receptor-mediated endocytosis (2012)
  • Author(s): Hanaki, H., et.al.
  • Journal Title: Mol.Cancer Ther. Volume: 11 Issue: 2 Pages: 298-307
  • DOI: 10.1158/1535-7163.mct-11-0682
  • Peer Reviewed
• [Journal Article] Wnt5a : its signaling, functions, and implication in diseases (2012)
  • Author(s): Kikuchi, A., et.al.
  • Journal Title: Acta Physiol. Volume: 204 Issue: 1 Pages: 17-33
  • DOI: 10.1111/j.1748-1716.2011.02294.x
  • Peer Reviewed
• [Journal Article] New insights into the mechanism of Wnt signaling pathway activation (2011)
  • Author(s): Kikuchi A., Yamamoto H., Sato A., Matsumoto S.
  • Journal Title: Int. Rev. Cell Mol. Biol Volume: 291 Pages: 21-71
  • Peer Reviewed
• [Journal Article] Wnt5a : its signalling, functions and implication in diseases (2011)
  • Author(s): Kikuchi A., Yamamoto H., Sato A., and Matsumoto S.
  • Journal Title: Acta Physiol.(Oxf)
  • Peer Reviewed
• [Journal Article] TRPM7 regulates gastrulation during vertebrate embryogenesis (2011)
  • Author(s): Liu W., Su L. T., Khadka D. K., Mezzacappa C., Komiya Y., Sato A., Habas R., and Runnels L. W.
  • Journal Title: Dev. Biol Volume: 350 Pages: 348-357
  • Peer Reviewed
• [Journal Article] New insights into the mechanism of Wnt signaling pathway activation (2011)
  • Author(s): Kikuchi, A
  • Journal Title: Int Rev Cell Mol Biol. Volume: 291 Pages: 21-71
  • DOI: 10.1016/b978-0-12-386035-4.00002-1
  • ISBN: 9780123860354
  • Peer Reviewed
• [Journal Article] TRPM7 regulates gastrulation during vertebrate embryogenesis. (2011)
  • Author(s): Liu, W., Sato, A., et al.
  • Journal Title: Developmental Biology Volume: 350 Pages: 348-357
  • Peer Reviewed
• [Journal Article] Wnt5a signaling is involved in the aggressiveness of prostate cancer and expression of metalloprotainase (2010)
  • Author(s): Yamamoto H., Oue N., Sato A., Hasegawa Y., Yamamoto H., Matsubara A., Yasui W, and Kikuchi A.
  • Journal Title: Oncogene Volume: 29 Pages: 2036-2046
  • Peer Reviewed
• [Journal Article] Wnt5a signaling is involved in the aggressiveness of prostate cancer and expression of metalloproteinase. (2010)
  • Author(s): Yamamoto, H., Sato, A., et al.
  • Journal Title: Oncogene Volume: 29 Pages: 2036-2046
  • Peer Reviewed
• [Presentation] Wnt5a deficiency ameliolates the dextran sulfate sodium(DSS)-induced acute colitis (2011)
  • Author(s): 佐藤朗
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] Wnt5a deficiency ameliolates the dextran sulfate sodium (DSS)-induced acute colitis (2011)
  • Author(s): 佐藤朗
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2011-12-14
• [Presentation] Wnt5a inhibits the Wnt/β-catenin pathway by binding to Frizzled2 (2010)
  • Author(s): 佐藤朗
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Presentation] Wnt5a inhibits the Wnt/β-catenin pathway by binding to Frizzled2. (2010)
  • Author(s): 佐藤朗
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
  • Year and Date: 2010-12-07
• [Remarks]
  • URL: http://www.med.osaka-u.ac.jp/pub/molbiobc/