Molecular mechanisms of mRNA decay that is triggered by translation arrest.

• Project/Area Number: 22770129
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: Nagoya City University
• Principal Investigator: HOSODA Nao 名古屋市立大学, 大学院・薬学研究科, 講師 (40438198)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000); Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: mRNA分解 / 翻訳制御 / G蛋白質 / リボソーム / mRNA品質管理

Research Abstract

In this study, we examined quality control mechanisms for mRNA lacking in-frame termination codons(non-stop mRNA) using mammalian cells. The non-stop mRNA is unstable and degraded in a translation-dependent manner. The decay requires another eRF3-eRF1 family member Hbs1-Dom34.Also, the elimination of aberrant proteins produced from the non-stop transcripts requires Listerin, which functions as an E3 ubiquitin ligase.

Research Products

• [Journal Article] Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase (2011)
  • Author(s): Nao Hosoda, Yuji Funakoshi, Masato Hirasawa, Ryota Yamagishi, Yukako Asano, Ryu Miyagawa, Koichi Ogami, Masafumi Tsujimoto, and Shin-ichi Hoshino
  • Journal Title: EMBO J Volume: 30 Pages: 1311-1323
• [Journal Article] Anti-proliferative protein Tob negatively regulates CPEB3 target by recruiting Caf1 deadenylase (2011)
  • Author(s): Nao Hosoda, Yuji Funakoshi, Masato Hirasawa, Ryota Yamagishi, Yukako Asano, Ryu Miyagawa, Koichi Ogami, Masafumi Tsujimoto, Shin-ichi Hoshino
  • Journal Title: EMBO J. Volume: 30 Issue: 7 Pages: 1311-1323
  • DOI: 10.1038/emboj.2011.37
  • Peer Reviewed
• [Journal Article] Quantitative Characterization of Tob Interactions Provides the Thermodynamic Basis for Translation Termination-coupled Deadenylase Regulation (2010)
  • Author(s): Lin Ruan, Masanori Osawa, Nao Hosoda, Shunsuke Imai, Asako Machiyama, Toshiaki Katada, Shin-ichi Hoshino, and Ichio Shimada
  • Journal Title: J. Biol. Chem Volume: 285(36) Pages: 27624-27631
• [Journal Article] Quantitative Characterization of Tob Interactions Provides the Thermodynamic Basis for Translation Termination-coupled Deadenylase Regulation. (2010)
  • Author(s): Lin Ruan, Masanori Osawa, Nao Hosoda, Shunsuke Imai, Asako Machiyama, Toshiaki Katada, Shin-ichi Hoshino, Ichio Shimada.
  • Journal Title: J.Biol.Chem. Volume: 285 Pages: 27624-27631
  • Peer Reviewed
• [Presentation] 癌抑制遺伝子産物Tobによるc-myc遺伝子の発現調節機構 (2012)
  • Author(s): 尾上耕一、細田直、船越祐司、星野真一
  • Organizer: 日本薬学会132年会
  • Place of Presentation: 札幌
• [Presentation] アポトーシス時における翻訳終結子eRF3のカスパーゼ依存的分解と翻訳制御 (2011)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: RNAフロンティアミーティング2011
  • Place of Presentation: 名古屋
• [Presentation] テロメラーゼRNA成熟化の分子機構 (2011)
  • Author(s): 田中麻記子、細田直、星野真一
  • Organizer: RNAフロンティアミーティング2011
  • Place of Presentation: 名古屋
• [Presentation] Anti-proliferative Protein Tob Negatively Regulates c-myc Oncogene Expression by Accelerating mRNA Deadenylation (2011)
  • Author(s): Koichi Ogami, Nao Hosoda, Yuji Funakoshi, Shin-ichi Hoshino
  • Organizer: RNA2011, the 16th Annual Meeting of the RNA Society
  • Place of Presentation: Kyoto
• [Presentation] PAM2モチーフ含有タンパク質USP10のストレス顆粒形成に果たす役割 (2011)
  • Author(s): 杉山遥、成瀬貴文、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
• [Presentation] テロメラーゼRNA(TLC1)の成熟化機構の解明 (2011)
  • Author(s): 田中麻記子、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 名古屋
• [Presentation] 癌抑制遺伝子産物Tobは癌原遺伝子c-mycの発現を負に制御する (2011)
  • Author(s): 尾上耕一、細田直、船越祐司、星野真一
  • Organizer: 日本薬学会131年会
  • Place of Presentation: 静岡
• [Presentation] カスパーゼによる翻訳終結子eRF3の分解と翻訳抑制 (2011)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: 日本薬学会131年会
  • Place of Presentation: 静岡
• [Presentation] アポトーシス時における翻訳終結因子eRF3のカスパーゼ依存的分解と翻訳制御 (2011)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: RNAフロンティアミーティング2011
  • Place of Presentation: 名古屋
• [Presentation] 抑制遺伝子産物TobはRas-MAPKシグナルの下流でc-myc mRNAの安定性を直接制御する (2010)
  • Author(s): 尾上耕一、細田直、船越祐司、星野真一
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京
  • Year and Date: 2010-07-28
• [Presentation] No go decay (NGD)によるmRNA品質管理機構の解析 (2010)
  • Author(s): 堀川桂、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 岐阜
  • Year and Date: 2010-07-03
• [Presentation] Anti-proliferative protein Tob nagatively regulates CPEB3 target by recruiting Caf1 deadenylase (2010)
  • Author(s): Nao Hosoda, Yuji Funakoshi, Masato Hirasawa, Ryota Yamagishi, Yukako Asano, Ryu Miyagawa, Koichi Ogami, Masafumi Tsujimoto, Shin-ichi Hoshino
  • Organizer: CSHL Meeting on Translational Control
  • Place of Presentation: Cold Spring Harbor, NY
• [Presentation] 癌抑制遺伝子産物TobはRas-MAPKシグナルの下流でc-myc mRNAの安定性を直接制御する (2010)
  • Author(s): 尾上耕一、細田直、船越祐司、星野真一
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京
• [Presentation] アポトーシス時における翻訳終結因子eRF3を標的とした翻訳制御 (2010)
  • Author(s): 橋本芳史、細田直、星野真一
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京
• [Presentation] 脊髄小脳変性症の原因遺伝子Ataxin-2がストレス顆粒形成に果たす役割 (2010)
  • Author(s): 成瀬貴文、的場洋子、細田直、星野真一
  • Organizer: 第12回日本RNA学会年会
  • Place of Presentation: 東京
• [Presentation] 酵母プリオン[PSI+]の表現型解析から見出した翻訳終結因子eRF3の新規機能 (2010)
  • Author(s): 岡本淳志、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 岐阜
• [Presentation] No go decay(NGD)によるmRNA品質管理機構の解析 (2010)
  • Author(s): 堀川桂、細田直、星野真一
  • Organizer: 日本薬学会東海支部大会
  • Place of Presentation: 岐阜
• [Remarks]
  • URL: http://www.phar.nagoya-cu.ac.jp/hp/syk/index.html