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Functional aspect of skeletal muscle-specific calpain system

Research Project

Project/Area Number 22770139
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeSingle-year Grants
Research Field Functional biochemistry
Research Institution公益財団法人東京都医学総合研究所 (2011)
Tokyo Metropolitan Organization for Medical Research (2010)

Principal Investigator

ONO Yasuko  公益財団法人東京都医学総合研究所, 生体分子先端研究分野, 主席研究員 (20392376)

Project Period (FY) 2010 – 2011
Project Status Completed (Fiscal Year 2011)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2010: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsカルパイン / 骨格筋 / プロテアーゼ / 自己消化活性 / サルコメア / 筋ジストロフィー / ノックインマウス / プロテオーム
Research Abstract

Regulatory mechanism of calpain3 (p94), a responsible gene product for LGMD2A, in skeletal muscle was investigated. It was demonstrated that Na^+ as well as Ca^<2+> activates calpain3. In addition, a novel protein, PLEIAD/SIMC1/C5orf25, was found to be a potential scaffold for calpain3.

Report

(3 results)
  • 2011 Annual Research Report   Final Research Report ( PDF )
  • 2010 Annual Research Report
  • Research Products

    (52 results)

All 2013 2012 2011 2010 Other

All Journal Article (18 results) (of which Peer Reviewed: 14 results) Presentation (24 results) Book (7 results) Remarks (3 results)

  • [Journal Article] PLEIAD/SIMC1/C5orf25, a Novel Autolysis Regulator for a Skeletal-Muscle-Specific Calpain, CAPN3, Scaffolds a CAPN3 Substrate, CTBP12013

    • Author(s)
      Ono, Y., Iemura, SI., Novak, S. M., Doi, N., Kitamura, F., Natsume, T., Gregorio, C. C., Sorimachi H
    • Journal Title

      J. Mol. Biol

      Volume: (in press) Issue: 16 Pages: 2955-2972

    • DOI

      10.1016/j.jmb.2013.05.009

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Understanding the substrate specificity of conventional calpains2012

    • Author(s)
      Sorimachi, H., Mamitsuka, H., Ono, Y
    • Journal Title

      Biol. Chem

      Volume: 393 Issue: 9 Pages: 853-871

    • DOI

      10.1515/hsz-2012-0143

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Regulation and physiological roles of the calpain system in muscular disorders2012

    • Author(s)
      Sorimachi, H., Ono, Y
    • Journal Title

      Cardiovasc. Res

      Volume: 96 Issue: 1 Pages: 11-22

    • DOI

      10.1093/cvr/cvs157

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calpains - An elaborate proteolytic system2012

    • Author(s)
      Ono Y, Sorimachi H
    • Journal Title

      Biochim Biophys Acta

      Volume: 1824 Issue: 1 Pages: 224-236

    • DOI

      10.1016/j.bbapap.2011.08.005

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Does the severity of the LGMD2A phenotype in compound heterozygotes depend on the combination of mutations?2011

    • Author(s)
      Saenz A, Ono Y, Sorimachi H, Goicoechea M, Leturcq F, Blazquez L, Garcia-Bragado F, Marina A, Poza JJ, Azpitarte M, Doi N, Urtasun M, Kaplan JC, Lopez de Munain A
    • Journal Title

      Muscle and Nerve

      Volume: 44 Issue: 5 Pages: 710-714

    • DOI

      10.1002/mus.22194

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calpain chronicle-an enzyme family under multidisciplinary characterization2011

    • Author(s)
      Sorimachi, H., Hata, S., Ono, Y
    • Journal Title

      Proc Jpn Acad Ser B Phys Biol Sci

      Volume: 87 Pages: 287-327

    • NAID

      130000903736

    • URL

      http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3153876/

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Impact of genetic insights into calpain biology2011

    • Author(s)
      Sorimachi H, Hata S, Ono Y
    • Journal Title

      J Biochem

      Volume: 150 Issue: 1 Pages: 23-37

    • DOI

      10.1093/jb/mvr070

    • NAID

      10030586192

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Calpain cleavage prediction using multiple kernel learning.2011

    • Author(s)
      duVerle DA, Ono Y, Sorimachi H, Mamitsuka H
    • Journal Title

      PLoS One

      Volume: 6 Issue: 5 Pages: e19035-e19035

    • DOI

      10.1371/journal.pone.0019035

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Non-proteolytic functions of calpain-3 in sarcoplasmic reticulum in skeletal muscles.2011

    • Author(s)
      Ojima K, Ono Y, Ottenheijm C, HataS, Suzuki H, Granzier H, Sorimachi H
    • Journal Title

      J Mol Biol

      Volume: 407 Issue: 3 Pages: 439-449

    • DOI

      10.1016/j.jmb.2011.01.057

    • Related Report
      2011 Annual Research Report 2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] 肢帯型筋ジストロフィーの発症機構2011

    • Author(s)
      反町洋之,小野弥子
    • Journal Title

      生体の科学

      Volume: 62-2 Pages: 95-99

    • Related Report
      2011 Final Research Report
  • [Journal Article] 骨格筋特異的カルパイン-3の活性制御因子は細胞内Na^+~例外か多様性か2011

    • Author(s)
      小野弥子,反町洋之
    • Journal Title

      化学と生物

      Volume: 49-4 Pages: 229-231

    • NAID

      10028117186

    • Related Report
      2011 Final Research Report
  • [Journal Article] カルパインの組織機能論2011

    • Author(s)
      反町洋之,秦勝志,小野弥子
    • Journal Title

      実験医学

      Volume: 29 Pages: 1882-1890

    • Related Report
      2011 Final Research Report
  • [Journal Article] Calpain chronicle-an enzyme family under multidisciplinary characterization.2011

    • Author(s)
      反町洋之
    • Journal Title

      Proc Jpn Acad Ser B Phys Biol Sci

      Volume: 87 Pages: 287-327

    • Related Report
      2011 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Expanding members and roles of the calpain superfamily and their genetically modified animals2010

    • Author(s)
      Sorimachi, H., Hata, S., and Ono, Y
    • Journal Title

      Exp. Anim

      Volume: 59 Pages: 549-566

    • NAID

      130000419250

    • URL

      https://www.jstage.jst.go.jp/article/expanim/59/5/59_5_549/_article

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Dynamic distribution of muscle-specific calpain in mice has a key role in physical-stress adaptation and is impaired in muscular dystrophy.2010

    • Author(s)
      Ojima K, Kawabata Y, Nakao H, Nakao K, Doi N, Kitamura F, Ono Y, HataS, Suzuki H, Kawahara H, Bogomolovas J,Will C, Ottenheijm C, LabeitS, Granzier H, Toyama-Sorimachi N, Sorimachi M, Suzuki K, Maeda T, Abe K, Aiba A, Sorimachi H
    • Journal Title

      J Clin Invest

      Volume: 120 Issue: 8 Pages: 2672-2683

    • DOI

      10.1172/jci40658

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Skeletal muscle-specific calpain is an intracellular Na^+-dependent protease.2010

    • Author(s)
      Ono Y, Ojima K, Torii F, Takaya E, Doi N, Nakagawa K, Hata S, Abe K, Sorimachi H
    • Journal Title

      J Biol Chem

      Volume: 285 Issue: 30 Pages: 22986-22998

    • DOI

      10.1074/jbc.m110.126946

    • Related Report
      2011 Final Research Report
    • Peer Reviewed
  • [Journal Article] Skeletal muscle-specific calpain is an intracellular Na^+-dependent protease2010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H.
    • Journal Title

      J.Biol.Chem.

      Volume: 285 Pages: 22986-22998

    • Related Report
      2010 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Redundant and non-redundant effects of Ca^<2+> and Na^+ on the activation of p94/calpain-32010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H.
    • Journal Title

      Neuromuscul.Disord.

      Volume: 20 Pages: 608-608

    • Related Report
      2010 Annual Research Report
  • [Presentation] 筋特異的カルパインCAPN3/p94活性化機構における分子間相補の解析 (Inter-molecular interaction involved in the activation of CAPN3/p94)2012

    • Author(s)
      小野弥子,田尾あすみ,北村ふじ子,反町洋之
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡
    • Related Report
      2011 Final Research Report
  • [Presentation] STRUCTURE-FUNCTION RELATIONSHIP OF Na^+-DEPENDENT ACTIVITY OF MUSCLE-SPECIFIC CALPAIN2012

    • Author(s)
      Ono,Y., Takaya, E., Doi, N., Kitamura, F., Sorimachi, H
    • Organizer
      SPIBC 2012(第13回プロテアーゼおよびその阻害剤と生体制御に関する国際会議)
    • Place of Presentation
      Portoroz, Slovenia
    • Related Report
      2011 Final Research Report
  • [Presentation] Characterization of sodium-dependent protease activity of muscle-specific calpain2012

    • Author(s)
      Ono,Y., Ouchi, F., Kitamura, F., Sorimachi, H
    • Organizer
      CAPN3. EMC2012(第41回ヨーロッパ筋機能研究学会)
    • Place of Presentation
      Rhodes, Greek
    • Related Report
      2011 Final Research Report
  • [Presentation] 筋特異的カルパインCAPN3/p94の新奇活性化機構の解析2011

    • Author(s)
      小野弥子,田尾あすみ,北村ふじ子,反町洋之
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Year and Date
      2011-12-14
    • Related Report
      2011 Final Research Report
  • [Presentation] Functional aspects of unique autolytic activity of CAPN32011

    • Author(s)
      Ono, Y
    • Organizer
      Department of Cellular and Molecular Medicine
    • Place of Presentation
      University of Arizona, Tucson, USA
    • Year and Date
      2011-02-11
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure-function relationship of Na^+-dependent activity of calpain-3/p942011

    • Author(s)
      Ono, Y
    • Organizer
      Korea-Japan symposium on protein metabolism
    • Place of Presentation
      Seoul National University, Seoul, Korea
    • Year and Date
      2011-01-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure-function relationship of Na^+-dependent activity of calpain-3/p942011

    • Author(s)
      Ono, Y.
    • Organizer
      Korea-Japan symposium on protein metabolism
    • Place of Presentation
      ソウル大学(韓国)(招待講演)
    • Year and Date
      2011-01-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Does the severity of the LGMD2A phenotype in compound heterozygotes depend on the combination of mutations?2011

    • Author(s)
      Ono, Y., Saenz, A., Sorimachi, H., Lopez de Munain, A
    • Organizer
      The 7th International Proteolysis Society meeting
    • Place of Presentation
      San Diego, USA
    • Related Report
      2011 Annual Research Report 2011 Final Research Report
  • [Presentation] Autolytic activity of skeletal muscle-specific calpain, CAPN3, diversifies its functions?2011

    • Author(s)
      Ono, Y., Doi, N., Kitamura, F., Sorimachi, H
    • Organizer
      CMM, MCB, and CBC joint retreat, Biosphere 2
    • Place of Presentation
      University of Arizona, Tucson, USA
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure-Function relationship of Na^+-dependent activity of calpain-3/p942011

    • Author(s)
      Ono, Y., Takaya, E., Doi, N., Kitamura, F., Sorimachi, H
    • Organizer
      The 17th Symposium on Ca^<2+>-Binding Proteins and Ca^<2+> Function in Health and Disease
    • Place of Presentation
      Beijing, China
    • Related Report
      2011 Final Research Report
  • [Presentation] Calpain-3/p94のNa依存性について2011

    • Author(s)
      小野弥子
    • Organizer
      平成22年度科学研究費補助金特定領域研究「タンパク質分解による細胞・個体機能の制御」班会議
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] Structure-Function relationship of Na+-dependent activity of calpain-3/p94.2011

    • Author(s)
      小野弥子
    • Organizer
      The 17th Symposium on Ca2+-Binding Proteins and Ca2+ Function in Health and Disease
    • Place of Presentation
      Beijing, China
    • Related Report
      2011 Annual Research Report
  • [Presentation] Calpain3/p94自己消化機構の解析2010

    • Author(s)
      小野弥子, 土井奈穂子, 高谷恵美, 北村ふじ子, 反町洋之
    • Organizer
      BMB2010(第33回日本分子生物学会年会・第83回日本生化学会大会合同大会)
    • Place of Presentation
      ポートピアホテル(神戸)
    • Year and Date
      2010-12-10
    • Related Report
      2010 Annual Research Report
  • [Presentation] Functions of calpain-3/p94 indicated by its unique properties2010

    • Author(s)
      Ono, Y
    • Organizer
      CHUV (Le Centre Hospitalier Universitaire Vaudois) Special Seminar
    • Place of Presentation
      Lausanne, Switzerland
    • Year and Date
      2010-10-01
    • Related Report
      2011 Final Research Report
  • [Presentation] Functions of calpain-3/p94 indicated by its unique properties2010

    • Author(s)
      Ono, Y.
    • Organizer
      CHUV (Le Centre Hospitalier Universitaire Vaudois) Special Seminar
    • Place of Presentation
      CHUV大学(スイス)(招待講演)
    • Year and Date
      2010-10-01
    • Related Report
      2010 Annual Research Report
  • [Presentation] Skeletal muscle-specific calpain, p94/calpain-3, is an intracellular Na^+-dependent protease2010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H.
    • Organizer
      XIIth Symposium on Proteases, Inhibitors and Biological Control
    • Place of Presentation
      Portoroz(スロベニア)
    • Year and Date
      2010-09-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] Characteristics of p94/calpain-3 as an intracellular Na^+-dependent protease2010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., and Sorimachi, H
    • Organizer
      FASEB Summer Research Conferences . Biology of the calpains in Health and Disease, Carefree, AZ
    • Place of Presentation
      USA
    • Year and Date
      2010-07-28
    • Related Report
      2011 Final Research Report
  • [Presentation] Characteristics of p94/calpain 3 as an intracellular Na^+-dependent protease2010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H.
    • Organizer
      FASEB Summer Research Conferences
    • Place of Presentation
      Carefree, AZ(米国)
    • Year and Date
      2010-07-28
    • Related Report
      2010 Annual Research Report
  • [Presentation] p94/calpain3自己消化機構の解析2010

    • Author(s)
      小野弥子、土井奈穂子、高谷恵美、北村ふじ子、反町洋之
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2010-06-18
    • Related Report
      2010 Annual Research Report
  • [Presentation] Calpain3/p94自己消化機構の解析2010

    • Author(s)
      小野弥子,土井奈穂子,高谷恵美,北村ふじ子,反町洋之
    • Organizer
      BMB2010 (第33回日本分子生物学会年会・第83回日本生化学会大会 合同大会)
    • Place of Presentation
      神戸
    • Related Report
      2011 Final Research Report
  • [Presentation] Redundant and non-redundant effects of Ca^<2+> and Na^+ on the activation of p94/calpain 32010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H
    • Organizer
      The 15th International Congress of the World Muscle Society
    • Place of Presentation
      Kumamoto, Japan
    • Related Report
      2011 Final Research Report
  • [Presentation] Skeletal muscle-specific calpain, p94/calpain-3, is an intracellular Na^+-dependent protease2010

    • Author(s)
      Ono, Y., Ojima, K., Torii, F., Takaya, E., Doi, N., Nakagawa, K., Hata, S., Abe, K., Sorimachi, H
    • Organizer
      XIIth Symposium on Proteases, Inhibitors and Biological Control
    • Place of Presentation
      Portoroz, Slovenia
    • Related Report
      2011 Final Research Report
  • [Presentation] p94/calpain3自己消化機構の解析, Inter/Intra molecular interaction involved in the autocatalytic proteolysis of p94/calpain 32010

    • Author(s)
      小野弥子,土井奈穂子,高谷恵美,北村ふじ子,反町洋之
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌
    • Related Report
      2011 Final Research Report
  • [Presentation] 筋特異的カルパインCAPN3/p94の新奇活性化機構の解析

    • Author(s)
      小野弥子
    • Organizer
      第34回日本分子生物学会年会
    • Place of Presentation
      横浜
    • Related Report
      2011 Annual Research Report
  • [Book] Chapter 471: μ-calpain, Chapter 472: m-calpain, Chapter 473: Muscle calpain, Chapter 474: Gastrointestinal calpain, Chapter 476: Other calpains. in: Handbook of Proteolytic enzymes, 3rd Edition. N.D. RawlIngs, G. Salvesen, editors2013

    • Author(s)
      Sorimachi, H., Hata, S., Ono, Y
    • Total Pages
      3932
    • Publisher
      Elsevier Academic Press (Oxford)
    • Related Report
      2011 Final Research Report
  • [Book] In "Encyclopedia of Biological Chemistry 2^<nd> Edition2013

    • Author(s)
      Sorimachi, H., Hata, S., and Ono, Y. Calpain
    • Total Pages
      3232
    • Publisher
      Elsevier, Oxford; eds., M.D. Lane and W. J. Lennarz
    • Related Report
      2011 Final Research Report
  • [Book] 「カルパイン」,モデル動物利用マニュアル…<生物機能モデルと新しいリソース・リサーチツール>2011

    • Author(s)
      反町洋之,秦勝志,小野弥子
    • Total Pages
      671
    • Publisher
      株式会社LIC
    • Related Report
      2011 Final Research Report
  • [Book] 肢帯型筋ジストロフィーの発症機構 (生体の科学 62-2)2011

    • Author(s)
      反町洋之
    • Total Pages
      5
    • Publisher
      医学書院
    • Related Report
      2011 Annual Research Report
  • [Book] 骨格筋特異的カルパイン-3の活性制御因子は細胞内Na+~例外か多様性か(化学と生物 49-4))2011

    • Author(s)
      小野弥子
    • Total Pages
      3
    • Publisher
      学会出版センター
    • Related Report
      2011 Annual Research Report
  • [Book] カルパインの組織機能論(実験医学 29)2011

    • Author(s)
      反町洋之
    • Total Pages
      9
    • Publisher
      羊土社
    • Related Report
      2011 Annual Research Report
  • [Book] カルパイン(生物機能モデルと新しいリソース・リサーチマニュアル)2011

    • Author(s)
      反町洋之
    • Total Pages
      17
    • Publisher
      株式会社LIC
    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.igakuken.or.jp/calpain/

    • Related Report
      2011 Final Research Report
  • [Remarks] 所属機関ホームページ

    • URL

      http://www.igakuken.or.jp/calpain/

    • Related Report
      2011 Annual Research Report
  • [Remarks]

    • URL

      http://www.rinshoken.or.jp/ER/

    • Related Report
      2010 Annual Research Report

URL: 

Published: 2010-08-23   Modified: 2016-04-21  

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