Characterization of the biosynthesis system for thiouridine in tRNA

• Project/Area Number: 22770140
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Functional biochemistry
• Research Institution: National Institute of Advanced Industrial Science and Technology
• Principal Investigator: SHIGI Naoki 独立行政法人産業技術総合研究所, バイオメディシナル情報研究センター, 研究員 (20392623)
• Project Period (FY): 2010 – 2011
• Project Status: Completed (Fiscal Year 2011)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2011: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2010: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 核酸 / タンパク質 / 酵素 / 微生物 / 蛋白質

Research Abstract

The biosynthesis pathway of thiouridine found in tRNA of thermophilic bacteria was analyzed. An in vitro reconstitution system for tRNA thiolation reaction was constructed. By using an in vivo system, a reaction mechanism for the thiolation enzyme TtuA was suggested. It was suggested that one of the biosynthesis protein, TtuB, also function as a protein modifier, which regulates the thiolation reaction of tRNA. This suggests an ancient origin of the eukaryotic ubiquitin system.

Research Products

• [Journal Article] Posttranslational modification of cellular proteins by a ubiquitin-like modifier in Bacteria (2012)
  • Author(s): 鴫直樹
  • Journal Title: Journal of Biological Chemistry Volume: Vol.287、No.21 Issue: 21 Pages: 17568-17577
  • DOI: 10.1074/jbc.m112.359844
  • Peer Reviewed
• [Journal Article] tRNAの硫黄修飾塩基の機能とその生合成機構 (2010)
  • Author(s): 鴫直樹
  • Journal Title: 生化学 Volume: Vol.82、No.7 Pages: 623-628
  • NAID: 10026607680
  • URL: http://wwwsoc.nii.ac.jp/jbiochem/magazine/82-07-07.pdf
  • Peer Reviewed
• [Presentation] Post-translational modification of cellular proteins by a ubiquitin-like modifier in eubacteria (2011)
  • Author(s): 鴫直樹
  • Organizer: EMBO Conference Series/Ubiquitin and ubiquitin-like modifiers
  • Place of Presentation: Cavtat(Croatia)
  • Year and Date: 2011-09-23
• [Presentation] Post-translational modification of cellular proteins by a ubiquitin-like modifier in eubacteria (2011)
  • Author(s): 鴫直樹
  • Organizer: EMBO Conference Series/Ubiquitin and ubiquitin-like modifier
  • Place of Presentation: Cavtat (Croatia)
  • Year and Date: 2011-09-23
• [Presentation] Biosynthesis of tRNA thiouridine in Thermus thermophilus and its evolutionary relationship with eukaryotic ubiquitin system (2011)
  • Author(s): 鴫直樹、坂口裕理子、朝井真一、鈴木勉、渡辺公綱
  • Organizer: 第16回RNA学会年会
  • Place of Presentation: 京都
  • Year and Date: 2011-06-17
• [Presentation] Biosynthesis of tRNA thiouridine in Thermus thermophilus and its evolutionary relationship with eukaryotic ubiquitin system (2011)
  • Author(s): 鴫直樹
  • Organizer: 第16回RNA学会年会
  • Place of Presentation: 京都国際会議場(京都府)
  • Year and Date: 2011-06-17
• [Presentation] tRNA硫黄修飾塩基の生合成機構の解析から明らかになったユビキチン修飾系との進化的関連 (2010)
  • Author(s): 鴫直樹、坂口裕理子、朝井真一、鈴木勉、渡辺公綱
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] tRNA硫黄修飾塩基の生合成機構の解析から明らかになったユビキチン修飾系との進化的関連 (2010)
  • Author(s): 鴫直樹
  • Organizer: BMB2010
  • Place of Presentation: 神戸ポートアイランド(神戸)
  • Year and Date: 2010-12-09
• [Presentation] 高度好熱菌tRNA硫黄修飾塩基の生合成機構の解析から明らかになった真核生物ユビキチン修飾系との進化的関連 (2010)
  • Author(s): 鴫直樹、坂口裕理子、朝井真一、鈴木勉、渡辺公綱
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第9回連携研究会
  • Place of Presentation: 兵庫県
  • Year and Date: 2010-08-22
• [Presentation] 高度好熱菌tRNA硫黄修飾塩基の生合成機構の解析から明らかになった真核生物ユビキチン修飾系との進化的関連 (2010)
  • Author(s): 鴫直樹
  • Organizer: 高度好熱菌丸ごと一匹プロジェクト第9回連携研究会
  • Place of Presentation: SPring8(兵庫県)
  • Year and Date: 2010-08-22
• [Presentation] 真正細菌tRNA修飾因子によるタンパク質翻訳後修飾の可能性 (2010)
  • Author(s): 鴫直樹
  • Organizer: 第12回 日本RNA学会年会
  • Place of Presentation: 一橋記念講堂(東京都)
  • Year and Date: 2010-07-27
• [Remarks]
  • URL: http://unit.aist.go.jp/birc2/RNA/index.html
• [Remarks]
  • URL: http://unit.aist.go.jp/birc2/RNA/index.html