| Project/Area Number |
22770147
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Nagoya University (2012-2013)
Kyoto University (2010-2011) |
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Principal Investigator |
TANI Kazutoshi 名古屋大学, 細胞生理学研究センター, 特任准教授 (20541204)
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| Co-Investigator(Renkei-kenkyūsha) |
FUJIYOSHI Yoshinori 名古屋大学, 細胞生理学研究センター, 特任教授 (80142298)
ABE Kazuhiro 名古屋大学, 細胞生理学研究センター, 助教 (60596188)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Project Status |
Completed (Fiscal Year 2013)
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| Budget Amount *help |
¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2012: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2011: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2010: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 高分解能電子顕微鏡解析 / 電子線結晶学 / 二次元結晶 / アクアポリン / aquaporin-4 / H+,K+-ATPase / 膜タンパク質 / 構造生物学 / 電子顕微鏡 / 三次元構造 / 水チャネル / アクアポリン-4 / H+, K+-ATPase / H+K+-ATPase |
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| Research Abstract |
Our developed software package for electron crystallography is very powerful and effective to determine three dimensional structures of membrane proteins reconstituted into lipid bilayers including aquaporin-4 and gastric H+,K+-ATPase. Using electron crystallography of two-dimensional crystals, we could elucidate their structures and conformational changes during molecular passages.
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