Time resolved infrared spectroscopy of cytochrome c oxidase in aqueous solution

Research Project

Project/Area Number	22770154
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Single-year Grants
Research Field	Biophysics
Research Institution	Okayama University of Science (2011) University of Hyogo (2010)
Principal Investigator	YAMAGUCHI Satoru 岡山理科大学, 理学部, 講師 (20347529)
Project Period (FY)	2010 – 2011
Project Status	Completed (Fiscal Year 2011)
Budget Amount *help	¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000) Fiscal Year 2011: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000) Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords	膜蛋白質 / 生体エネルギー / 赤外分光 / プロトンポンプ / 赤外分光法 / マイクロチップ / 生体エネルギー変換 / 構造生物学
Research Abstract	The newly developed time-resolved infrared system was constructed using fs infrared laser for a light source to investigate a proton pumping mechanism of cytochrome c oxidase(C_cO). The function of the O_2 reduction site using CO as an analogue of O_2 was investigated by this new system which is capable of analyzing C_cO in H_2O solution. The results indicate the existence of a conformational relay system which plays a key role in connecting the CuB site and the water channel for efficient collection of protons being pumped. It was found that transient CO-binding to Cu_B, after Fe_<a3>-CO photolysis, opens the water-channel.