| Project/Area Number |
22770162
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Biophysics
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| Research Institution | Keio University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2011
|
| Project Status |
Completed (Fiscal Year 2011)
|
| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2011: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2010: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
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| Keywords | 銅シャペロン蛋白質 / SOD1 / ジスルフィド結合 / 蛋白質間分子認識 |
|---|
| Research Abstract |
To reveal a molecular mechanism regulating the intracellular transport of copper ions, I have noted specific interactions between a copper chaperone, CCS, and a copper-requiring enzyme, SOD1. Inside cells, a copper-bound form of CCS specifically recognizes apo SOD1 and then delivers a copper ion to SOD1. CCS consists of three domains, among which a central domain(CCS-dII) plays a role in recognizing SOD1. In this study, I have performed in vitro biochemical tests using recombinant CCS-dII and SOD1 proteins and found the requirements for these two proteins to associate with each other.
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