| Budget Amount *help |
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
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| Research Abstract |
As there was a variety of difficulties in the course of the study, including a low expression level of proteins in in vitro transcription/translation system, I conducted two researches: one is a study using directed evolution as I originally planned, and the other one is the study of creating a new function by using a DNA scaffold to reconstitute a complex of several motor proteins. In the former study, I created molecules with interesting properties by directed evolution (the patent application is under consideration). In the latter study, I revealed that cooperative, collective behavior of motor protein complexes is highly dependent on the type of motor proteins.
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