Molecular design of motor proteins by directed evolution

• Project/Area Number: 22770164
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Single-year Grants
• Research Field: Biophysics
• Research Institution: National Institute of Information and Communications Technology
• Principal Investigator: FURUTA Kenya 独立行政法人情報通信研究機構, 未来ICT研究所 バイオICT研究室, 主任研究員 (40571831)
• Project Period (FY): 2010-04-01 – 2014-03-31
• Project Status: Completed (Fiscal Year 2013)
• Budget Amount: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2012: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2011: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2010: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: キネシン / 進化分子工学 / 分子モーター / 低分子GTPase / 進化 / 低分子GTPアーゼ

Research Abstract

As there was a variety of difficulties in the course of the study, including a low expression level of proteins in in vitro transcription/translation system, I conducted two researches: one is a study using directed evolution as I originally planned, and the other one is the study of creating a new function by using a DNA scaffold to reconstitute a complex of several motor proteins. In the former study, I created molecules with interesting properties by directed evolution (the patent application is under consideration). In the latter study, I revealed that cooperative, collective behavior of motor protein complexes is highly dependent on the type of motor proteins.

Research Products

• [Journal Article] Coupling of Two Non-processive Myosin 5c Dimers Enables Processive Stepping along Actin Filaments (2014)
  • Author(s): Laura K. Gunther, 古田 健也, Jianjun Bao, Monica K. Urbanowski, Howard D. White, 小嶋 寛明, Takeshi Sakamoto
  • Journal Title: Scientific Reports Volume: Vol.4 Issue: 1 Pages: 4907-4907
  • DOI: 10.1038/srep04907
  • Peer Reviewed
• [Journal Article] バイオイメージングと光ピンセットを用いた微小管系モータータンパク質の協同的活性化に関する解析 (2014)
  • Author(s): 古田 健也, 鳥澤 嵩征, 豊島 陽子
  • Journal Title: 生化学 Volume: Vol.86 No.2 Pages: 184-191
  • URL: http://www.bioweb.ne.jp/content/seika/seika1402.html
• [Journal Article] バイオイメージングと光ピンセットを用いた微小管系モータータンパク質の協同的活性化に関する解析 (2014)
  • Author(s): 古田 健也，鳥澤 嵩征，豊島 陽子
  • Journal Title: 生化学 Volume: 86 Pages: 184-191
• [Journal Article] Measuring Collective Transport by Defined Numbers of Processive and Nonprocessive Kinesin Motors (2013)
  • Author(s): 古田 健也, 古田 茜, 豊島 陽子, 網野 美紗子, 大岩 和弘, 小嶋 寛明
  • Journal Title: Proc Natl Acad Sci U S A ( 米国科学アカデミー紀要) Volume: 110(2) Issue: 2 Pages: 501-6
  • DOI: 10.1073/pnas.1201390110
  • Peer Reviewed
• [Presentation] 細胞質ダイニンの自己阻害と協同的な活性化 (2014)
  • Author(s): 古田 健也，鳥澤 嵩征，古田 茜，市川 宗厳，斎藤 慧，大岩 和弘，小嶋 寛明，豊島 陽子
  • Organizer: 生体運動合同班会議
  • Place of Presentation: 千葉大学，千葉県千葉市
• [Presentation] Self-regulation of cytoplasmic dynein (2013)
  • Author(s): 古田 健也, 鳥澤 嵩征, 古田 茜, 市川 宗厳, 斎藤 慧, 大岩 和弘, 小嶋 寛明, 豊島 陽子
  • Organizer: 2013 ASCB Annual Meeting
  • Place of Presentation: New Orleans Ernest N. Morial Convention Center, New Orleans, LA, USA
  • Year and Date: 2013-12-17
• [Presentation] Self-regulation of cytoplasmic dynein (2013)
  • Author(s): 古田 健也
  • Organizer: Dynein 2013 International Workshop
  • Place of Presentation: 神戸ファッション美術館, 兵庫県神戸市
  • Year and Date: 2013-11-01
• [Presentation] Self-regulation of cytoplasmic dynein (2013)
  • Author(s): 古田 健也
  • Organizer: Dynein 2013 International Workshop
  • Place of Presentation: 神戸ファッション美術館，兵庫県神戸市
  • Invited
• [Presentation] モータータンパク質集合体の自己組織化を操る (2013)
  • Author(s): 古田 健也
  • Organizer: 日本生物物理学会 第51回年会
  • Place of Presentation: 国立京都国際会館，京都府京都市
• [Presentation] 再構成実験とシミュレーションによる分子モーターのアンサンブル特性の探究 (2011)
  • Author(s): 古田健也
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 兵庫県立大学書写キャンパス,姫路,兵庫県
  • Year and Date: 2011-09-16
• [Presentation] Molecular-scale communication systems developed by combination of DNA and protein motors (2011)
  • Author(s): 古田健也
  • Organizer: THERMEC '2011
  • Place of Presentation: Quebec City Convention Centre, Quebec, Canada
  • Year and Date: 2011-08-05
• [Presentation] COOPERATIVE TRANSPORT BY DEFINED NUMBERS OF PROCESSIVE AND NONPROCESSIVE KINESIN MOTORS (2011)
  • Author(s): 古田 健也, 豊島 陽子, 小嶋 寛明, 大岩 和弘
  • Organizer: ゴードン研究会議
  • Place of Presentation: Colby-Sawyer College, New London, USA
  • Year and Date: 2011-07-12
• [Presentation] COOPERATIVE TRANSPORT BY DEFINED NUMBERS OF PROCESSIVE AND NONPROCESSIVE KINESIN MOTORS (2011)
  • Author(s): 古田健也
  • Organizer: ゴードン研究会議
  • Place of Presentation: Colby-Sawyer College, New London, USA(招待講演)
  • Year and Date: 2011-07-12
• [Presentation] RECONSTITUTION OF COLLECTIVE TRANSPORT BY DEFINED NUMBERS OF KINESIN-1 AND KINESIN-14, TWO OPPOSING MOTOR PROTEINS (2011)
  • Author(s): 古田 健也, 豊島 陽子, 小嶋 寛明, 大岩 和弘
  • Organizer: 米国生物物理学会第55回年会
  • Place of Presentation: Baltimore Convention Center, Baltimore, Maryland, USA
  • Year and Date: 2011-03-06
• [Presentation] 微小管系分子モーターの複数分子による機能を計測する
  • Author(s): 古田 健也
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋大学・愛知県
  • Invited
• [Presentation] Measuring Collective Transport by Defined Numbers of Processive and Nonprocessive Kinesin Motor
  • Author(s): Ken'ya Furuta
  • Organizer: The Biophysical Society, 57th Annual Meeting
  • Place of Presentation: Pennsylvania Convention Center, USA